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Protein

Protein lysine acetyltransferase Pka

Gene

pka

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acetylates RNase R in exponential phase cells. Also required for the glucose-dependent acetylation on multiple lysines of alpha, beta and beta' RNAP subunits. May acetylate Acs and inhibit its activity.4 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • cofactor binding Source: InterPro
  • metal ion binding Source: InterPro
  • peptide-lysine-N-acetyltransferase activity Source: EcoCyc
  • peptidyl-lysine acetyltransferase activity Source: EcoliWiki

GO - Biological processi

  • internal peptidyl-lysine acetylation Source: EcoCyc
  • protein homotetramerization Source: EcoCyc
  • regulation of protein homooligomerization Source: EcoCyc
  • response to heat Source: EcoCyc
  • response to oxidative stress Source: EcoCyc

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G7350-MONOMER
MetaCyc:G7350-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lysine acetyltransferase Pka (EC:2.3.1.-)
Gene namesi
Name:pka
Synonyms:patZ, yfiQ
Ordered Locus Names:b2584, JW2568
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14224 pka

Pathology & Biotechi

Disruption phenotypei

Deletion mutant has decreased resistance to oxidative and heat stress. Mutant responds poorly to glucose.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001692721 – 886Protein lysine acetyltransferase PkaAdd BLAST886

Proteomic databases

PaxDbiP76594
PRIDEiP76594

Expressioni

Inductioni

Positively regulated by cAMP-CRP. Up-regulated in the presence of non-PTS carbon sources (PubMed:22059728). According to PubMed:22059728, pka is up-regulated during the stationary phase growth, while according to PubMed:22124017, it is absent from the late exponential and stationary phase cells.2 Publications

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260608, 12 interactors
DIPiDIP-12068N
IntActiP76594, 30 interactors
STRINGi316385.ECDH10B_2752

Structurei

3D structure databases

ProteinModelPortaliP76594
SMRiP76594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini487 – 523ATP-graspPROSITE-ProRule annotationAdd BLAST37
Domaini726 – 881N-acetyltransferasePROSITE-ProRule annotationAdd BLAST156

Sequence similaritiesi

In the N-terminal section; belongs to the acetate CoA ligase alpha subunit family.Curated
In the central section; belongs to the acetate CoA ligase beta subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105CD5 Bacteria
COG0454 LUCA
COG1042 LUCA
HOGENOMiHOG000220090
InParanoidiP76594
KOiK09181
OMAiHWEADVV
PhylomeDBiP76594

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.40.50.261, 2 hits
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR003781 CoA-bd
IPR000182 GNAT_dom
IPR036291 NAD(P)-bd_dom_sf
IPR032875 Succ_CoA_lig_flav_dom
IPR016102 Succinyl-CoA_synth-like
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
PF13380 CoA_binding_2, 1 hit
PF13607 Succ_CoA_lig, 1 hit
SMARTiView protein in SMART
SM00881 CoA_binding, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52210 SSF52210, 2 hits
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

P76594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRGLEALL RPKSIAVIGA SMKPNRAGYL MMRNLLAGGF NGPVLPVTPA
60 70 80 90 100
WKAVLGVLAW PDIASLPFTP DLAVLCTNAS RNLALLEELG EKGCKTCIIL
110 120 130 140 150
SAPASQHEDL RACALRHNMR LLGPNSLGLL APWQGLNASF SPVPIKRGKL
160 170 180 190 200
AFISQSAAVS NTILDWAQQR KMGFSYFIAL GDSLDIDVDE LLDYLARDSK
210 220 230 240 250
TSAILLYLEQ LSDARRFVSA ARSASRNKPI LVIKSGRSPA AQRLLNTTAG
260 270 280 290 300
MDPAWDAAIQ RAGLLRVQDT HELFSAVETL SHMRPLRGDR LMIISNGAAP
310 320 330 340 350
AALALDALWS RNGKLATLSE ETCQKLRDAL PEHVAISNPL DLRDDASSEH
360 370 380 390 400
YIKTLDILLH SQDFDALMVI HSPSAAAPAT ESAQVLIEAV KHHPRSKYVS
410 420 430 440 450
LLTNWCGEHS SQEARRLFSE AGLPTYRTPE GTITAFMHMV EYRRNQKQLR
460 470 480 490 500
ETPALPSNLT SNTAEAHLLL QQAIAEGATS LDTHEVQPIL QAYGMNTLPT
510 520 530 540 550
WIASDSTEAV HIAEQIGYPV ALKLRSPDIP HKSEVQGVML YLRTANEVQQ
560 570 580 590 600
AANAIFDRVK MAWPQARVHG LLVQSMANRA GAQELRVVVE HDPVFGPLIM
610 620 630 640 650
LGEGGVEWRP EDQAVVALPP LNMNLARYLV IQGIKSKKIR ARSALRPLDV
660 670 680 690 700
AGLSQLLVQV SNLIVDCPEI QRLDIHPLLA SGSEFTALDV TLDISPFEGD
710 720 730 740 750
NESRLAVRPY PHQLEEWVEL KNGERCLFRP ILPEDEPQLQ QFISRVTKED
760 770 780 790 800
LYYRYFSEIN EFTHEDLANM TQIDYDREMA FVAVRRIDQT EEILGVTRAI
810 820 830 840 850
SDPDNIDAEF AVLVRSDLKG LGLGRRLMEK LITYTRDHGL QRLNGITMPN
860 870 880
NRGMVALARK LGFNVDIQLE EGIVGLTLNL AQREES
Length:886
Mass (Da):97,987
Last modified:February 1, 1997 - v1
Checksum:i255944B9E2961251
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti506 – 507ST → YA in BAA10925 (Ref. 3) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75637.1
AP009048 Genomic DNA Translation: BAE76754.1
D64044 Genomic DNA Translation: BAA10925.1
PIRiG65036
RefSeqiNP_417079.1, NC_000913.3
WP_000083005.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75637; AAC75637; b2584
BAE76754; BAE76754; BAE76754
GeneIDi947056
KEGGiecj:JW2568
eco:b2584
PATRICifig|1411691.4.peg.4150

Similar proteinsi

Entry informationi

Entry nameiPKA_ECOLI
AccessioniPrimary (citable) accession number: P76594
Secondary accession number(s): Q2MAF2, Q47320
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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