P76578 (YFHM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized lipoprotein YfhM

Gene names

Name:	yfhM
Ordered Locus Names:	b2520, JW2504

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	1653 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Subcellular location

Cell membrane; Lipid-anchor Potential.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Coiled coil Signal
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to external side of plasma membrane Inferred from direct assay PubMed 18697741. Source: EcoCyc
Molecular_function	endopeptidase inhibitor activity Inferred from direct assay PubMed 18697741. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 1653	1636	Uncharacterized lipoprotein YfhM		PRO_0000013775
Regions
	Coiled coil	1559 – 1589	31	Potential
Amino acid modifications
	Lipidation	18	1	N-palmitoyl cysteine Potential
	Lipidation	18	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	P76578 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 13109EC5CDEB41A0	FASTA	1,653	181,585
	10 20 30 40 50 60 MKKLRVAACM LMLALAGCDN NDNAPTAVKK DAPSEVTKAA SSENASSAKL SVPERQKLAQ 70 80 90 100 110 120 QSAGKVLTLL DLSEVQLDGA ATLVLTFSIP LDPDQDFSRV IHVVDKKSGK VDGAWELSDN 130 140 150 160 170 180 LKELRLRHLE PKRDLIVTIG KEVKALNNAT FSKDYEKTIT TRDIQPSVGF ASRGSLLPGK 190 200 210 220 230 240 VVEGLPVMAL NVNNVDVNFF RVKPESLPAF ISQWEYRNSL ANWQSDKLLQ MADLVYTGRF 250 260 270 280 290 300 DLNPARNTRE KLLLPLGDIK PLQQAGVYLA VMNQAGRYDY SNPATLFTLS DIGVSAHRYH 310 320 330 340 350 360 NRLDIFTQSL ENGAAQQGIE VSLLNEKGQT LTQATSDAQG HVQLENDKNA ALLLARKDGQ 370 380 390 400 410 420 TTLLDLKLPA LDLAEFNIAG APGYSKQFFM FGPRDLYRPG ETVILNGLLR DADGKALPNQ 430 440 450 460 470 480 PIKLDVIKPD GQVLRSVVSQ PENGLYHFTW PLDSNAATGM WHIRANTGDN QYRMWDFHVE 490 500 510 520 530 540 DFMPERMALN LTGEKTPLTP KDEVKFSVVG YYLYGAPANG NTLQGQLFLR PLREAVSALP 550 560 570 580 590 600 GFEFGDIAAE NLSRTLDEVQ LTLDDKGRGE VSTESQWKET HSPLQVIFQG SLLESGGRPV 610 620 630 640 650 660 TRRAEQAIWP ADALPGIRPQ FASKSVYDYR TDSTVKQPIV DEGSNAAFDI VYSDAQGVKK 670 680 690 700 710 720 AVSGLQVRLI RERRDYYWNW SEDEGWQSQF DQKDLIENEQ TLDLKADETG KVSFPVEWGA 730 740 750 760 770 780 YRLEVKAPNE AVSSVRFWAG YSWQDNSDGS GAVRPDRVTL KLDKASYRPG DTIKLHIAAP 790 800 810 820 830 840 TAGKGYAMVE SSEGPLWWQE IDVRAQGLDL TIPVDKTWNR HDLYLSTLVV RPGDKSRSAT 850 860 870 880 890 900 PKRAVGVLHL PLGDENRRLD LALETPAKMR PNQPLTVKIK ASTKNGEKPK QVNVLVSAVD 910 920 930 940 950 960 SGVLNITDYV TPDPWQAFFG QKRYGADIYD IYGQVIEGQG RLAALRFGGD GDELKRGGKP 970 980 990 1000 1010 1020 PVNHVNIVVQ QALPVTLNEQ GEGSVTLPIG DFNGELRVMA QAWTADDFGS NESKVIVAAP 1030 1040 1050 1060 1070 1080 VIAELNMPRF MASGDTSRLT LDITNLTDKP QKLNVALTAS GLLELVSDSP AAVELAPGVR 1090 1100 1110 1120 1130 1140 TTLFIPVRAL PGYGDGEIQA TISGLALPGE TVADQHKQWK IGVRPAFPAQ TVNYGTALQP 1150 1160 1170 1180 1190 1200 GETWAIPADG LQNFSPVTLE GQLLLSGKPP LNIARYIKEL KAYPYGCLEQ TASGLFPSLY 1210 1220 1230 1240 1250 1260 TNAAQLQALG IKGDSDEKRR ASVDIGISRL LQMQRDNGGF ALWDKNGDEE YWLTAYVMDF 1270 1280 1290 1300 1310 1320 LVRAGEQGYS VPTDAINRGN ERLLRYLQDP GMMSIPYADN LKASKFAVQS YAALVLARQQ 1330 1340 1350 1360 1370 1380 KAPLGALREI WEHRADAASG LPLLQLGVAL KTMGDATRGE EAIALALKTP RNSDERIWLG 1390 1400 1410 1420 1430 1440 DYGSSLRDNA LMLSLLEENK LLPDEQYTLL NTLSQQAFGE RWLSTQESNA LFLAARTIQD 1450 1460 1470 1480 1490 1500 LPGKWQAQTS FSAEQLTGEK AQNSNLNSDQ LVTLQVSNSG DQPLWLRMDA SGYPQSAPLP 1510 1520 1530 1540 1550 1560 ANNVLQIERH ILGTDGKSKS LDSLRSGDLV LVWLQVKASN SVPDALVVDL LPAGLELENQ 1570 1580 1590 1600 1610 1620 NLANGSASLE QSGGEVQNLL NQMQQASIKH IEFRDDRFVA AVAVDEYQPV TLVYLARAVT 1630 1640 1650 PGTYQVPQPM VESMYVPQWR ATGAAEDLLI VRP

References

[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75573.1. AP009048 Genomic DNA. Translation: BAE76729.1.
PIR	G65028.
RefSeq	NP_417015.1. NC_000913.2. YP_490748.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76578.
SMR	P76578. Positions 389-521.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-28064N.
IntAct	P76578. 15 interactions.
STRING	511145.b2520.
Protein family/group databases
MEROPS	I39.008.
Proteomic databases
PaxDb	P76578.
PRIDE	P76578.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75573; AAC75573; b2520. BAE76729; BAE76729; BAE76729.
GeneID	12931809. 947302.
KEGG	ecj:Y75_p2473. eco:b2520.
PATRIC	32120433. VBIEscCol129921_2619.
Organism-specific databases
EchoBASE	EB3175.
EcoGene	EG13394. yfhM.
Phylogenomic databases
eggNOG	COG2373.
HOGENOM	HOG000275932.
KO	K06894.
OMA	DGPLWWQ.
ProtClustDB	CLSK880420.
Enzyme and pathway databases
BioCyc	EcoCyc:G7323-MONOMER. ECOL316407:JW2504-MONOMER.
Gene expression databases
Genevestigator	P76578.
Family and domain databases
InterPro	IPR026284. A2-macglob_dom_prot_bac. IPR002890. A2M_N. IPR011625. A2M_N_2. IPR021868. Alpha_2_Macroglob_MG1. IPR001599. Macroglobln_a2. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00207. A2M. 1 hit. PF01835. A2M_N. 1 hit. PF07703. A2M_N_2. 1 hit. PF11974. MG1. 1 hit. [Graphical view]
PIRSF	PIRSF038980. A2M_bac. 1 hit.
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YFHM_ECOLI

Accession

Primary (citable) accession number: P76578
Secondary accession number(s): Q2MAH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program