Reviewed, UniProtKB/Swiss-Prot P76558 (MAO2_ECOLI)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:	maeB
Synonyms:	ypfF
Ordered Locus Names:	b2463, JW2447

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homooligomer.
Sequence similarities	In the N-terminal section; belongs to the malic enzymes family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-545413,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	NADP-dependent malic enzyme		PRO_0000160242
Regions
	Nucleotide binding	76 – 83	8	NADP By similarity
	Region	1 – 428	428	Malic enzyme
	Region	429 – 759	331	Phosphate acetyltransferase
Sites
	Active site	94	1	Proton acceptor By similarity
	Metal binding	136	1	Divalent metal cation By similarity
	Metal binding	137	1	Divalent metal cation By similarity
	Binding site	162	1	NAD By similarity
	Binding site	288	1	NAD By similarity
Amino acid modifications
	Modified residue	56	1	N6-acetyllysine

Sequences

	Sequence		Length	Mass (Da)
	P76558-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 9A8B67D9635E25BA	FASTA	759	82,417
	10 20 30 40 50 60 MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLKAYKY 70 80 90 100 110 120 TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK FAGIDVFDIE VDELDPDKFI 130 140 150 160 170 180 EVVAALEPTF GGINLEDIKA PECFYIEQKL RERMNIPVFH DDQHGTAIIS TAAILNGLRV 190 200 210 220 230 240 VEKNISDVRM VVSGAGAAAI ACMNLLVALG LQKHNIVVCD SKGVIYQGRE PNMAETKAAY 250 260 270 280 290 300 AVVDDGKRTL DDVIEGADIF LGCSGPKVLT QEMVKKMARA PMILALANPE PEILPPLAKE 310 320 330 340 350 360 VRPDAIICTG RSDYPNQVNN VLCFPFIFRG ALDVGATAIN EEMKLAAVRA IAELAHAEQS 370 380 390 400 410 420 EVVASAYGDQ DLSFGPEYII PKPFDPRLIV KIAPAVAKAA MESGVATRPI ADFDVYIDKL 430 440 450 460 470 480 TEFVYKTNLF MKPIFSQARK APKRVVLPEG EEARVLHATQ ELVTLGLAKP ILIGRPNVIE 490 500 510 520 530 540 MRIQKLGLQI KAGVDFEIVN NESDPRFKEY WTEYFQIMKR RGVTQEQAQR ALISNPTVIG 550 560 570 580 590 600 AIMVQRGEAD AMICGTVGDY HEHFSVVKNV FGYRDGVHTA GAMNALLLPS GNTFIADTYV 610 620 630 640 650 660 NDEPDAEELA EITLMAAETV RRFGIEPRVA LLSHSNFGSS DCPSSSKMRQ ALELVRERAP 670 680 690 700 710 720 ELMIDGEMHG DAALVEAIRN DRMPDSSLKG SANILVMPNM EAARISYNLL RVSSSEGVTV 730 740 750 GPVLMGVAKP VHVLTPIASV RRIVNMVALA VVEAQTQPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Studies on regulatory functions of malic enzymes. VI. Purification and molecular properties of NADP-linked malic enzyme from Escherichia coli W." Iwakura M., Hattori J., Arita Y., Tokushige M., Katsuki H. J. Biochem. 85:1355-1365(1979) [PubMed: 36376] [Abstract] Cited for: CHARACTERIZATION. Strain: W / ATCC 11105 / DSM 1900.
[5]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in E. coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 0:0-0(2008) [PubMed: 18723842] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-56, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC75516.1. AP009048 Genomic DNA. Translation: BAA16337.1.
PIR	F65021.
RefSeq	AP_003048.1. NP_416958.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:10141N.
IntAct	P76558.
Genome annotation databases
GeneID	946947.
GenomeReviews	Gene locus b2463 in contig U00096_GR. Gene locus JW2447 in contig AP009048_GR.
KEGG	ecj:JW2447. eco:b2463.
Organism-specific databases
EchoBASE	EB3945.
EcoGene	EG14193. maeB.
CMR	Search...
Phylogenomic databases
HOGENOM	P76558.
Enzyme and pathway databases
BioCyc	EcoCyc:MALIC-NADP-MON. MetaCyc:MALIC-NADP-MON.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR012188. ME_PTA. IPR016040. NAD(P)-bd. IPR002505. PTA_PTB. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF036684. ME_PTA. 1 hit.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

MAO2_ECOLI

Accession

Primary (citable) accession number: P76558
Secondary accession number(s): P78200, P78201

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	November 4, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)