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Protein

Probable deferrochelatase/peroxidase YfeX

Gene

yfeX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the tetrapyrrol ring intact.1 Publication

Cofactori

heme b1 PublicationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi215 – 2151Iron (heme proximal ligand); via tele nitrogenCurated

GO - Molecular functioni

  • heme binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7266-MONOMER.
ECOL316407:JW2424-MONOMER.
MetaCyc:G7266-MONOMER.

Protein family/group databases

PeroxiBasei5875. EcoDyPrx02_K12.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable deferrochelatase/peroxidase YfeX (EC:1.11.1.-)
Gene namesi
Name:yfeX
Ordered Locus Names:b2431, JW2424
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14165. yfeX.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi215 – 2151H → A: Strong decrease in affinity for heme and protoporphyrin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Probable deferrochelatase/peroxidase YfeXPRO_0000201328Add
BLAST

Proteomic databases

PaxDbiP76536.
PRIDEiP76536.

Interactioni

Protein-protein interaction databases

BioGridi4260737. 14 interactions.
DIPiDIP-47971N.
IntActiP76536. 4 interactions.
STRINGi511145.b2431.

Structurei

3D structure databases

ProteinModelPortaliP76536.
SMRiP76536. Positions 1-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DyP-type peroxidase family.Curated

Phylogenomic databases

eggNOGiENOG4105EZR. Bacteria.
COG2837. LUCA.
HOGENOMiHOG000151980.
InParanoidiP76536.
KOiK07223.
OMAiCAEPNLH.
OrthoDBiEOG6N682X.
PhylomeDBiP76536.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR006314. Dyp_peroxidase.
[Graphical view]
PfamiPF04261. Dyp_perox. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR01413. Dyp_perox_fam. 1 hit.
PROSITEiPS51404. DYP_PEROXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVQSGILP EHCRAAIWIE ANVKGEVDAL RAASKTFADK LATFEAKFPD
60 70 80 90 100
AHLGAVVAFG NNTWRALSGG VGAEELKDFP GYGKGLAPTT QFDVLIHILS
110 120 130 140 150
LRHDVNFSVA QAAMEAFGDC IEVKEEIHGF RWVEERDLSG FVDGTENPAG
160 170 180 190 200
EETRREVAVI KDGVDAGGSY VFVQRWEHNL KQLNRMSVHD QEMVIGRTKE
210 220 230 240 250
ANEEIDGDER PETSHLTRVD LKEDGKGLKI VRQSLPYGTA SGTHGLYFCA
260 270 280 290
YCARLHNIEQ QLLSMFGDTD GKRDAMLRFT KPVTGGYYFA PSLDKLMAL
Length:299
Mass (Da):33,052
Last modified:March 27, 2002 - v2
Checksum:iB64D99408BB9126F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75484.2.
AP009048 Genomic DNA. Translation: BAE76711.1.
PIRiF65017.
RefSeqiNP_416926.4. NC_000913.3.
WP_001350538.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75484; AAC75484; b2431.
BAE76711; BAE76711; BAE76711.
GeneIDi946913.
KEGGiecj:JW2424.
eco:b2431.
PATRICi32120247. VBIEscCol129921_2526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75484.2.
AP009048 Genomic DNA. Translation: BAE76711.1.
PIRiF65017.
RefSeqiNP_416926.4. NC_000913.3.
WP_001350538.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76536.
SMRiP76536. Positions 1-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260737. 14 interactions.
DIPiDIP-47971N.
IntActiP76536. 4 interactions.
STRINGi511145.b2431.

Protein family/group databases

PeroxiBasei5875. EcoDyPrx02_K12.

Proteomic databases

PaxDbiP76536.
PRIDEiP76536.

Protocols and materials databases

DNASUi946913.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75484; AAC75484; b2431.
BAE76711; BAE76711; BAE76711.
GeneIDi946913.
KEGGiecj:JW2424.
eco:b2431.
PATRICi32120247. VBIEscCol129921_2526.

Organism-specific databases

EchoBASEiEB3917.
EcoGeneiEG14165. yfeX.

Phylogenomic databases

eggNOGiENOG4105EZR. Bacteria.
COG2837. LUCA.
HOGENOMiHOG000151980.
InParanoidiP76536.
KOiK07223.
OMAiCAEPNLH.
OrthoDBiEOG6N682X.
PhylomeDBiP76536.

Enzyme and pathway databases

BioCyciEcoCyc:G7266-MONOMER.
ECOL316407:JW2424-MONOMER.
MetaCyc:G7266-MONOMER.

Miscellaneous databases

PROiP76536.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR006314. Dyp_peroxidase.
[Graphical view]
PfamiPF04261. Dyp_perox. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR01413. Dyp_perox_fam. 1 hit.
PROSITEiPS51404. DYP_PEROXIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Bacteria capture iron from heme by keeping tetrapyrrol skeleton intact."
    Letoffe S., Heuck G., Delepelaire P., Lange N., Wandersman C.
    Proc. Natl. Acad. Sci. U.S.A. 106:11719-11724(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DEFERROCHELATASE, COFACTOR, SUBCELLULAR LOCATION, MUTAGENESIS OF HIS-215.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiYFEX_ECOLI
AccessioniPrimary (citable) accession number: P76536
Secondary accession number(s): Q2MAJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: July 6, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.