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Protein

Probable deferrochelatase/peroxidase YfeX

Gene

yfeX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the tetrapyrrol ring intact.1 Publication

Cofactori

heme b1 PublicationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi215Iron (heme proximal ligand); via tele nitrogenCurated1

GO - Molecular functioni

  • heme binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase, Peroxidase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7266-MONOMER.
MetaCyc:G7266-MONOMER.

Protein family/group databases

PeroxiBasei5875. EcoDyPrx02_K12.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable deferrochelatase/peroxidase YfeX (EC:1.11.1.-)
Gene namesi
Name:yfeX
Ordered Locus Names:b2431, JW2424
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14165. yfeX.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi215H → A: Strong decrease in affinity for heme and protoporphyrin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002013281 – 299Probable deferrochelatase/peroxidase YfeXAdd BLAST299

Proteomic databases

PaxDbiP76536.
PRIDEiP76536.

Interactioni

Protein-protein interaction databases

BioGridi4260737. 14 interactors.
DIPiDIP-47971N.
IntActiP76536. 4 interactors.
STRINGi511145.b2431.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 23Combined sources10
Helixi27 – 29Combined sources3
Helixi30 – 47Combined sources18
Helixi49 – 51Combined sources3
Beta strandi54 – 59Combined sources6
Helixi61 – 68Combined sources8
Beta strandi70 – 72Combined sources3
Turni83 – 86Combined sources4
Beta strandi93 – 101Combined sources9
Helixi103 – 117Combined sources15
Turni118 – 120Combined sources3
Beta strandi121 – 130Combined sources10
Helixi133 – 135Combined sources3
Beta strandi140 – 142Combined sources3
Helixi151 – 158Combined sources8
Turni164 – 167Combined sources4
Beta strandi169 – 178Combined sources10
Helixi180 – 183Combined sources4
Helixi188 – 195Combined sources8
Turni199 – 201Combined sources3
Helixi215 – 219Combined sources5
Beta strandi235 – 238Combined sources4
Beta strandi240 – 242Combined sources3
Beta strandi244 – 253Combined sources10
Helixi255 – 265Combined sources11
Beta strandi268 – 271Combined sources4
Helixi275 – 278Combined sources4
Beta strandi280 – 282Combined sources3
Beta strandi286 – 290Combined sources5
Turni293 – 297Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GT2X-ray2.09A/B/C/D1-299[»]
ProteinModelPortaliP76536.
SMRiP76536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DyP-type peroxidase family.Curated

Phylogenomic databases

eggNOGiENOG4105EZR. Bacteria.
COG2837. LUCA.
HOGENOMiHOG000151980.
InParanoidiP76536.
KOiK07223.
OMAiCAEPNLH.
PhylomeDBiP76536.

Family and domain databases

InterProiView protein in InterPro
IPR011008. Dimeric_a/b-barrel.
IPR006314. Dyp_peroxidase.
PfamiView protein in Pfam
PF04261. Dyp_perox. 1 hit.
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR01413. Dyp_perox_fam. 1 hit.
PROSITEiView protein in PROSITE
PS51404. DYP_PEROXIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P76536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVQSGILP EHCRAAIWIE ANVKGEVDAL RAASKTFADK LATFEAKFPD
60 70 80 90 100
AHLGAVVAFG NNTWRALSGG VGAEELKDFP GYGKGLAPTT QFDVLIHILS
110 120 130 140 150
LRHDVNFSVA QAAMEAFGDC IEVKEEIHGF RWVEERDLSG FVDGTENPAG
160 170 180 190 200
EETRREVAVI KDGVDAGGSY VFVQRWEHNL KQLNRMSVHD QEMVIGRTKE
210 220 230 240 250
ANEEIDGDER PETSHLTRVD LKEDGKGLKI VRQSLPYGTA SGTHGLYFCA
260 270 280 290
YCARLHNIEQ QLLSMFGDTD GKRDAMLRFT KPVTGGYYFA PSLDKLMAL
Length:299
Mass (Da):33,052
Last modified:March 27, 2002 - v2
Checksum:iB64D99408BB9126F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75484.2.
AP009048 Genomic DNA. Translation: BAE76711.1.
PIRiF65017.
RefSeqiNP_416926.4. NC_000913.3.
WP_001350538.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75484; AAC75484; b2431.
BAE76711; BAE76711; BAE76711.
GeneIDi946913.
KEGGiecj:JW2424.
eco:b2431.
PATRICi32120247. VBIEscCol129921_2526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75484.2.
AP009048 Genomic DNA. Translation: BAE76711.1.
PIRiF65017.
RefSeqiNP_416926.4. NC_000913.3.
WP_001350538.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GT2X-ray2.09A/B/C/D1-299[»]
ProteinModelPortaliP76536.
SMRiP76536.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260737. 14 interactors.
DIPiDIP-47971N.
IntActiP76536. 4 interactors.
STRINGi511145.b2431.

Protein family/group databases

PeroxiBasei5875. EcoDyPrx02_K12.

Proteomic databases

PaxDbiP76536.
PRIDEiP76536.

Protocols and materials databases

DNASUi946913.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75484; AAC75484; b2431.
BAE76711; BAE76711; BAE76711.
GeneIDi946913.
KEGGiecj:JW2424.
eco:b2431.
PATRICi32120247. VBIEscCol129921_2526.

Organism-specific databases

EchoBASEiEB3917.
EcoGeneiEG14165. yfeX.

Phylogenomic databases

eggNOGiENOG4105EZR. Bacteria.
COG2837. LUCA.
HOGENOMiHOG000151980.
InParanoidiP76536.
KOiK07223.
OMAiCAEPNLH.
PhylomeDBiP76536.

Enzyme and pathway databases

BioCyciEcoCyc:G7266-MONOMER.
MetaCyc:G7266-MONOMER.

Miscellaneous databases

PROiPR:P76536.

Family and domain databases

InterProiView protein in InterPro
IPR011008. Dimeric_a/b-barrel.
IPR006314. Dyp_peroxidase.
PfamiView protein in Pfam
PF04261. Dyp_perox. 1 hit.
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR01413. Dyp_perox_fam. 1 hit.
PROSITEiView protein in PROSITE
PS51404. DYP_PEROXIDASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYFEX_ECOLI
AccessioniPrimary (citable) accession number: P76536
Secondary accession number(s): Q2MAJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: May 10, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.