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Protein

Aminopeptidase YpdF

Gene

ypdF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the N-terminal methionine when the next amino acid is alanine, proline or serine. The substrate preference for methionyl aminopeptidase activity is Pro > Ala > Ser. Also able to hydrolyze the Xaa-Pro peptide bond when the first amino acid is alanine, asparagine or methionine.1 Publication

Cofactori

Co2+, Mn2+, Ni2+Note: Co2+, Mn2+ or Ni2+.

GO - Molecular functioni

  • aminopeptidase activity Source: EcoCyc

GO - Biological processi

  • proteolysis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Enzyme and pathway databases

BioCyciEcoCyc:G7248-MONOMER.
ECOL316407:JW2382-MONOMER.
MetaCyc:G7248-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase YpdF (EC:3.4.11.-)
Gene namesi
Name:ypdF
Ordered Locus Names:b2385, JW2382
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14153. ypdF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Aminopeptidase YpdFPRO_0000185105Add
BLAST

Proteomic databases

PaxDbiP76524.
PRIDEiP76524.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F52EBI-1128711,EBI-543750

Protein-protein interaction databases

BioGridi4259189. 12 interactions.
DIPiDIP-48229N.
IntActiP76524. 1 interaction.
STRINGi511145.b2385.

Structurei

3D structure databases

ProteinModelPortaliP76524.
SMRiP76524. Positions 1-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24 family.Curated

Phylogenomic databases

eggNOGiENOG4105DC7. Bacteria.
COG0006. LUCA.
HOGENOMiHOG000008760.
InParanoidiP76524.
KOiK08326.
OMAiSHILEFI.
OrthoDBiEOG618QTW.
PhylomeDBiP76524.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.

Sequencei

Sequence statusi: Complete.

P76524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLASLRDW LKAQQLDAVL LSSRQNKQPH LGISTGSGYV VISRESAHIL
60 70 80 90 100
VDSRYYVEVE ARAQGYQLHL LDATNTLTTI VNQIIADEQL QTLGFEGQQV
110 120 130 140 150
SWETAHRWQS ELNAKLVSAT PDVLRQIKTP EEVEKIRLAC GIADRGAEHI
160 170 180 190 200
RRFIQAGMSE REIAAELEWF MRQQGAEKAS FDTIVASGWR GALPHGKASD
210 220 230 240 250
KIVAAGEFVT LDFGALYQGY CSDMTRTLLV NGEGVSAESH LLFNVYQIVL
260 270 280 290 300
QAQLAAISAI RPGVRCQQVD DAARRVITEA GYGDYFGHNT GHAIGIEVHE
310 320 330 340 350
DPRFSPRDTT TLQPGMLLTV EPGIYLPGQG GVRIEDVVLV TPQGAEVLYA
360
MPKTVLLTGE A
Length:361
Mass (Da):39,624
Last modified:February 1, 1997 - v1
Checksum:i34E97443005EB8D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75444.1.
AP009048 Genomic DNA. Translation: BAA16255.2.
PIRiF65012.
RefSeqiNP_416886.1. NC_000913.3.
WP_000173288.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75444; AAC75444; b2385.
BAA16255; BAA16255; BAA16255.
GeneIDi946853.
KEGGiecj:JW2382.
eco:b2385.
PATRICi32120149. VBIEscCol129921_2483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75444.1.
AP009048 Genomic DNA. Translation: BAA16255.2.
PIRiF65012.
RefSeqiNP_416886.1. NC_000913.3.
WP_000173288.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76524.
SMRiP76524. Positions 1-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259189. 12 interactions.
DIPiDIP-48229N.
IntActiP76524. 1 interaction.
STRINGi511145.b2385.

Proteomic databases

PaxDbiP76524.
PRIDEiP76524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75444; AAC75444; b2385.
BAA16255; BAA16255; BAA16255.
GeneIDi946853.
KEGGiecj:JW2382.
eco:b2385.
PATRICi32120149. VBIEscCol129921_2483.

Organism-specific databases

EchoBASEiEB3905.
EcoGeneiEG14153. ypdF.

Phylogenomic databases

eggNOGiENOG4105DC7. Bacteria.
COG0006. LUCA.
HOGENOMiHOG000008760.
InParanoidiP76524.
KOiK08326.
OMAiSHILEFI.
OrthoDBiEOG618QTW.
PhylomeDBiP76524.

Enzyme and pathway databases

BioCyciEcoCyc:G7248-MONOMER.
ECOL316407:JW2382-MONOMER.
MetaCyc:G7248-MONOMER.

Miscellaneous databases

PROiP76524.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Characterization of two new aminopeptidases in Escherichia coli."
    Zheng Y., Roberts R.J., Kasif S., Guan C.
    J. Bacteriol. 187:3671-3677(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiYPDF_ECOLI
AccessioniPrimary (citable) accession number: P76524
Secondary accession number(s): P76951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.