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Protein

Probable phospholipid-binding lipoprotein MlaA

Gene

mlaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.1 Publication

Enzyme and pathway databases

BioCyciEcoCyc:G7216-MONOMER.
ECOL316407:JW2343-MONOMER.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-binding lipoprotein MlaA
Gene namesi
Name:mlaA
Synonyms:vacJ
Ordered Locus Names:b2346, JW2343
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14276. mlaA.

Subcellular locationi

  • Cell outer membrane 1 Publication; Lipid-anchor PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutation confers sensitivity to SDS-EDTA and leads to accumulation of phospholipid in the outer leaflet of the outer membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717PROSITE-ProRule annotationAdd
BLAST
Chaini18 – 251234Probable phospholipid-binding lipoprotein MlaAPRO_0000018210Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi18 – 181N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi18 – 181S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP76506.
PRIDEiP76506.

Interactioni

Protein-protein interaction databases

BioGridi4260538. 150 interactions.
IntActiP76506. 4 interactions.
STRINGi511145.b2346.

Structurei

3D structure databases

ProteinModelPortaliP76506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MlaA family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410903C. Bacteria.
COG2853. LUCA.
HOGENOMiHOG000220801.
InParanoidiP76506.
KOiK04754.
OMAiFQRYDFL.
OrthoDBiEOG6677N2.
PhylomeDBiP76506.

Family and domain databases

InterProiIPR007428. MlaA.
[Graphical view]
PANTHERiPTHR30035. PTHR30035. 1 hit.
PfamiPF04333. MlaA. 1 hit.
[Graphical view]
PRINTSiPR01805. VACJLIPOPROT.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRLSALAL GTTLLVGCAS SGTDQQGRSD PLEGFNRTMY NFNFNVLDPY
60 70 80 90 100
IVRPVAVAWR DYVPQPARNG LSNFTGNLEE PAVMVNYFLQ GDPYQGMVHF
110 120 130 140 150
TRFFLNTILG MGGFIDVAGM ANPKLQRTEP HRFGSTLGHY GVGYGPYVQL
160 170 180 190 200
PFYGSFTLRD DGGDMADGFY PVLSWLTWPM SVGKWTLEGI ETRAQLLDSD
210 220 230 240 250
GLLRQSSDPY IMVREAYFQR HDFIANGGEL KPQENPNAQA IQDDLKDIDS

E
Length:251
Mass (Da):28,042
Last modified:February 1, 1997 - v1
Checksum:iF008CD42CB8C75B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 44MKLR → YEAS no nucleotide entry (PubMed:9205837).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75406.1.
AP009048 Genomic DNA. Translation: BAA16206.2.
PIRiH65007.
RefSeqiNP_416848.1. NC_000913.3.
WP_000776765.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75406; AAC75406; b2346.
BAA16206; BAA16206; BAA16206.
GeneIDi945582.
KEGGiecj:JW2343.
eco:b2346.
PATRICi32120065. VBIEscCol129921_2442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75406.1.
AP009048 Genomic DNA. Translation: BAA16206.2.
PIRiH65007.
RefSeqiNP_416848.1. NC_000913.3.
WP_000776765.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260538. 150 interactions.
IntActiP76506. 4 interactions.
STRINGi511145.b2346.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP76506.
PRIDEiP76506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75406; AAC75406; b2346.
BAA16206; BAA16206; BAA16206.
GeneIDi945582.
KEGGiecj:JW2343.
eco:b2346.
PATRICi32120065. VBIEscCol129921_2442.

Organism-specific databases

EchoBASEiEB4024.
EcoGeneiEG14276. mlaA.

Phylogenomic databases

eggNOGiENOG410903C. Bacteria.
COG2853. LUCA.
HOGENOMiHOG000220801.
InParanoidiP76506.
KOiK04754.
OMAiFQRYDFL.
OrthoDBiEOG6677N2.
PhylomeDBiP76506.

Enzyme and pathway databases

BioCyciEcoCyc:G7216-MONOMER.
ECOL316407:JW2343-MONOMER.

Miscellaneous databases

PROiP76506.

Family and domain databases

InterProiIPR007428. MlaA.
[Graphical view]
PANTHERiPTHR30035. PTHR30035. 1 hit.
PfamiPF04333. MlaA. 1 hit.
[Graphical view]
PRINTSiPR01805. VACJLIPOPROT.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 1-4.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane."
    Malinverni J.C., Silhavy T.J.
    Proc. Natl. Acad. Sci. U.S.A. 106:8009-8014(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHOLIPID TRANSPORT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100 / JA176.

Entry informationi

Entry nameiMLAA_ECOLI
AccessioniPrimary (citable) accession number: P76506
Secondary accession number(s): P76941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.