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Protein

3-ketoacyl-CoA thiolase

Gene

fadI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids.2 Publications

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Acyl-thioester intermediateBy similarity
Active sitei392 – 3921Proton acceptorBy similarity
Active sitei422 – 4221Proton acceptorBy similarity

GO - Molecular functioni

  • 3-hydroxyacyl-CoA dehydrogenase activity Source: EcoliWiki
  • acetyl-CoA C-acyltransferase activity Source: EcoliWiki
  • enoyl-CoA hydratase activity Source: EcoliWiki

GO - Biological processi

  • fatty acid beta-oxidation, unsaturated, even number Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:G7213-MONOMER.
ECOL316407:JW2339-MONOMER.
MetaCyc:G7213-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase (EC:2.3.1.16)
Alternative name(s):
ACSs
Acetyl-CoA acyltransferase
Acyl-CoA ligase
Beta-ketothiolase
Fatty acid oxidation complex subunit beta
Gene namesi
Name:fadI
Synonyms:yfcY
Ordered Locus Names:b2342, JW2339
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14128. fadI.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4364363-ketoacyl-CoA thiolasePRO_0000206437Add
BLAST

Proteomic databases

EPDiP76503.
PaxDbiP76503.
PRIDEiP76503.

Expressioni

Inductioni

Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).By similarity

Protein-protein interaction databases

BioGridi4260534. 173 interactions.
DIPiDIP-11991N.
IntActiP76503. 12 interactions.
STRINGi511145.b2342.

Structurei

3D structure databases

ProteinModelPortaliP76503.
SMRiP76503. Positions 17-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
HOGENOMiHOG000012240.
InParanoidiP76503.
KOiK00632.
OMAiMTAFPEP.
PhylomeDBiP76503.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
HAMAPiMF_01618. FadI. 1 hit.
InterProiIPR012806. Ac-CoA_C-AcTrfase_FadI.
IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
TIGR02446. FadI. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQVLPLVTR QGDRIAIVSG LRTPFARQAT AFHGIPAVDL GKMVVGELLA
60 70 80 90 100
RSEIPAEVIE QLVFGQVVQM PEAPNIAREI VLGTGMNVHT DAYSVSRACA
110 120 130 140 150
TSFQAVANVA ESLMAGTIRA GIAGGADSSS VLPIGVSKKL ARVLVDVNKA
160 170 180 190 200
RTMSQRLKLF SRLRLRDLMP VPPAVAEYST GLRMGDTAEQ MAKTYGITRE
210 220 230 240 250
QQDALAHRSH QRAAQAWSDG KLKEEVMTAF IPPYKQPLVE DNNIRGNSSL
260 270 280 290 300
ADYAKLRPAF DRKHGTVTAA NSTPLTDGAA AVILMTESRA KELGLVPLGY
310 320 330 340 350
LRSYAFTAID VWQDMLLGPA WSTPLALERA GLTMSDLTLI DMHEAFAAQT
360 370 380 390 400
LANIQLLGSE RFAREALGRA HATGEVDDSK FNVLGGSIAY GHPFAATGAR
410 420 430
MITQTLHELR RRGGGFGLVT ACAAGGLGAA MVLEAE
Length:436
Mass (Da):46,531
Last modified:February 1, 1997 - v1
Checksum:i5B89853172C16299
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75402.1.
AP009048 Genomic DNA. Translation: BAA16197.2.
PIRiD65007.
RefSeqiNP_416844.1. NC_000913.3.
WP_000531952.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75402; AAC75402; b2342.
BAA16197; BAA16197; BAA16197.
GeneIDi948823.
KEGGiecj:JW2339.
eco:b2342.
PATRICi32120057. VBIEscCol129921_2438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75402.1.
AP009048 Genomic DNA. Translation: BAA16197.2.
PIRiD65007.
RefSeqiNP_416844.1. NC_000913.3.
WP_000531952.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76503.
SMRiP76503. Positions 17-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260534. 173 interactions.
DIPiDIP-11991N.
IntActiP76503. 12 interactions.
STRINGi511145.b2342.

Proteomic databases

EPDiP76503.
PaxDbiP76503.
PRIDEiP76503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75402; AAC75402; b2342.
BAA16197; BAA16197; BAA16197.
GeneIDi948823.
KEGGiecj:JW2339.
eco:b2342.
PATRICi32120057. VBIEscCol129921_2438.

Organism-specific databases

EchoBASEiEB3880.
EcoGeneiEG14128. fadI.

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
HOGENOMiHOG000012240.
InParanoidiP76503.
KOiK00632.
OMAiMTAFPEP.
PhylomeDBiP76503.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciEcoCyc:G7213-MONOMER.
ECOL316407:JW2339-MONOMER.
MetaCyc:G7213-MONOMER.

Miscellaneous databases

PROiP76503.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
HAMAPiMF_01618. FadI. 1 hit.
InterProiIPR012806. Ac-CoA_C-AcTrfase_FadI.
IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
TIGR02446. FadI. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADI_ECOLI
AccessioniPrimary (citable) accession number: P76503
Secondary accession number(s): P77208, P77230, Q6KCX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.