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Protein

Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF

Gene

arnF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the periplasmic side of the inner membrane.1 Publication

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • substrate-specific transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • lipid A biosynthetic process Source: UniProtKB-HAMAP
  • lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • response to iron(III) ion Source: EcoCyc
  • transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7171-MONOMER.
ECOL316407:JW5373-MONOMER.
MetaCyc:G7171-MONOMER.
UniPathwayiUPA00030.

Protein family/group databases

TCDBi2.A.7.22.1. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF
Short name:
L-Ara4N-phosphoundecaprenol flippase subunit ArnF
Alternative name(s):
Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF
Gene namesi
Name:arnF
Synonyms:pmrM, yfbJ
Ordered Locus Names:b2258, JW5373
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14094. arnF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22CytoplasmicSequence analysis
Transmembranei3 – 2321HelicalSequence analysisAdd
BLAST
Topological domaini24 – 3512PeriplasmicSequence analysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Topological domaini57 – 7620CytoplasmicSequence analysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence analysisAdd
BLAST
Topological domaini98 – 1003PeriplasmicSequence analysis
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Topological domaini122 – 1287CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are polymyxin sensitive. Lipid A is no longer modified with L-Ara4N even though the level of the lipid-linked donor of the L-Ara4N moiety, alpha-L-Ara4N-phosphoundecaprenol, is not reduced. However, the alpha-L-Ara4N-phosphoundecaprenol is less concentrated on the periplasmic surface of the inner membrane when compared to wild type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128128Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnFPRO_0000218152Add
BLAST

Proteomic databases

PaxDbiP76474.

Expressioni

Inductioni

Induced by BasR.1 Publication

Interactioni

Subunit structurei

Heterodimer of ArnE and ArnF.1 Publication

Protein-protein interaction databases

BioGridi4260501. 651 interactions.
DIPiDIP-48225N.
STRINGi511145.b2258.

Structurei

3D structure databases

ProteinModelPortaliP76474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ArnF family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105U65. Bacteria.
COG0697. LUCA.
HOGENOMiHOG000261165.
InParanoidiP76474.
KOiK12963.
OMAiVWLISRP.

Family and domain databases

HAMAPiMF_00538. Flippase_ArnF. 1 hit.
InterProiIPR022832. Flippase_ArnF.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76474-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLMWGLFSV IIASVAQLSL GFAASHLPPM THLWDFIAAL LAFGLDARIL
60 70 80 90 100
LLGLLGYLLS VFCWYKTLHK LALSKAYALL SMSYVLVWIA SMVLPGWEGT
110 120
FSLKALLGVA CIMSGLMLIF LPTTKQRY
Length:128
Mass (Da):14,085
Last modified:February 21, 2002 - v2
Checksum:i1ACEEE17FDF6B446
GO

Sequence cautioni

The sequence AAB04894 differs from that shown. Reason: Frameshift at position 128. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35031 Genomic DNA. Translation: AAB04894.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75318.2.
AP009048 Genomic DNA. Translation: BAE76676.1.
PIRiH64996.
RefSeqiNP_416761.4. NC_000913.3.
WP_000523880.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75318; AAC75318; b2258.
BAE76676; BAE76676; BAE76676.
GeneIDi945344.
KEGGiecj:JW5373.
eco:b2258.
PATRICi32119883. VBIEscCol129921_2351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35031 Genomic DNA. Translation: AAB04894.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75318.2.
AP009048 Genomic DNA. Translation: BAE76676.1.
PIRiH64996.
RefSeqiNP_416761.4. NC_000913.3.
WP_000523880.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260501. 651 interactions.
DIPiDIP-48225N.
STRINGi511145.b2258.

Protein family/group databases

TCDBi2.A.7.22.1. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbiP76474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75318; AAC75318; b2258.
BAE76676; BAE76676; BAE76676.
GeneIDi945344.
KEGGiecj:JW5373.
eco:b2258.
PATRICi32119883. VBIEscCol129921_2351.

Organism-specific databases

EchoBASEiEB3847.
EcoGeneiEG14094. arnF.

Phylogenomic databases

eggNOGiENOG4105U65. Bacteria.
COG0697. LUCA.
HOGENOMiHOG000261165.
InParanoidiP76474.
KOiK12963.
OMAiVWLISRP.

Enzyme and pathway databases

UniPathwayiUPA00030.
BioCyciEcoCyc:G7171-MONOMER.
ECOL316407:JW5373-MONOMER.
MetaCyc:G7171-MONOMER.

Miscellaneous databases

PROiP76474.

Family and domain databases

HAMAPiMF_00538. Flippase_ArnF. 1 hit.
InterProiIPR022832. Flippase_ArnF.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARNF_ECOLI
AccessioniPrimary (citable) accession number: P76474
Secondary accession number(s): Q2MAN0, Q47376
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: February 21, 2002
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.