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Protein

Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

Gene

arnT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.1 Publication

Catalytic activityi

4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + lipid IV(A) = lipid II(A) + di-trans,octa-cis-undecaprenyl phosphate.1 Publication

Pathwayi: 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis

This protein is involved in the pathway 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis, which is part of Lipopolysaccharide metabolism.
View all proteins of this organism that are known to be involved in the pathway 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis and in Lipopolysaccharide metabolism.

GO - Molecular functioni

GO - Biological processi

  • lipid A biosynthetic process Source: UniProtKB-KW
  • lipopolysaccharide biosynthetic process Source: EcoCyc
  • protein O-linked glycosylation Source: InterPro
  • response to iron(III) ion Source: EcoCyc

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7170-MONOMER
MetaCyc:G7170-MONOMER
BRENDAi2.4.2.43 2026
UniPathwayiUPA00037

Protein family/group databases

CAZyiGT83 Glycosyltransferase Family 83
TCDBi9.B.142.2.9 the integral membrane glycosyltransferase family 39 (gt39) family

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (EC:2.4.2.43)
Alternative name(s):
4-amino-4-deoxy-L-arabinose lipid A transferase
Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase
Polymyxin resistance protein PmrK
Undecaprenyl phosphate-alpha-L-Ara4N transferase
Gene namesi
Name:arnT
Synonyms:pmrK, yfbI
Ordered Locus Names:b2257, JW2251
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14093 arnT

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Transmembranei110 – 131HelicalSequence analysisAdd BLAST22
Transmembranei137 – 154HelicalSequence analysisAdd BLAST18
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Transmembranei315 – 335HelicalSequence analysisAdd BLAST21
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Transmembranei406 – 426HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215041 – 550Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferaseAdd BLAST550

Proteomic databases

PaxDbiP76473
PRIDEiP76473

Expressioni

Inductioni

Induced by BasR.By similarity

Interactioni

Protein-protein interaction databases

BioGridi4260499, 153 interactors
STRINGi316385.ECDH10B_2417

Structurei

3D structure databases

ProteinModelPortaliP76473
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 83 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105SA9 Bacteria
COG1807 LUCA
HOGENOMiHOG000273002
InParanoidiP76473
KOiK07264
OMAiTFWPGAP
PhylomeDBiP76473

Family and domain databases

HAMAPiMF_01165 ArnT_transfer, 1 hit
InterProiView protein in InterPro
IPR022839 ArnT_tfrase
IPR003342 Glyco_trans_39/83
PfamiView protein in Pfam
PF02366 PMT, 1 hit

Sequencei

Sequence statusi: Complete.

P76473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVRYLIGL FAFIACYYLL PISTRLLWQP DETRYAEISR EMLASGDWIV
60 70 80 90 100
PHLLGLRYFE KPIAGYWINS IGQWLFGANN FGVRAGVIFA TLLTAALVTW
110 120 130 140 150
FTLRLWRDKR LALLATVIYL SLFIVYAIGT YAVLDPFIAF WLVAGMCSFW
160 170 180 190 200
LAMQAQTWKG KSAGFLLLGI TCGMGVMTKG FLALAVPVLS VLPWVATQKR
210 220 230 240 250
WKDLFIYGWL AVISCVLTVL PWGLAIAQRE PNFWHYFFWV EHIQRFALDD
260 270 280 290 300
AQHRAPFWYY VPVIIAGSLP WLGLLPGALY TGWKNRKHSA TVYLLSWTIM
310 320 330 340 350
PLLFFSVAKG KLPTYILSCF ASLAMLMAHY ALLAAKNNPL ALRINGWINI
360 370 380 390 400
AFGVTGIIAT FVVSPWGPMN TPVWQTFESY KVFCAWSIFS LWAFFGWYTL
410 420 430 440 450
TNVEKTWPFA ALCPLGLALL VGFSIPDRVM EGKHPQFFVE MTQESLQPSR
460 470 480 490 500
YILTDSVGVA AGLAWSLQRD DIIMYRQTGE LKYGLNYPDA KGRFVSGDEF
510 520 530 540 550
ANWLNQHRQE GIITLVLSVD RDEDINSLAI PPADAIDRQE RLVLIQYRPK
Length:550
Mass (Da):62,543
Last modified:February 1, 1997 - v1
Checksum:iE57E0C3A0608D745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75317.1
AP009048 Genomic DNA Translation: BAE76674.1
PIRiG64996
RefSeqiNP_416760.1, NC_000913.3
WP_000844057.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75317; AAC75317; b2257
BAE76674; BAE76674; BAE76674
GeneIDi947297
KEGGiecj:JW2251
eco:b2257
PATRICifig|511145.12.peg.2349

Similar proteinsi

Entry informationi

Entry nameiARNT_ECOLI
AccessioniPrimary (citable) accession number: P76473
Secondary accession number(s): Q2MAN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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