Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

Gene

arnT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.1 Publication

Catalytic activityi

4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + lipid IV(A) = lipid II(A) + di-trans,octa-cis-undecaprenyl phosphate.1 Publication

Pathwayi: 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis

This protein is involved in the pathway 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis, which is part of Lipopolysaccharide metabolism.
View all proteins of this organism that are known to be involved in the pathway 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis and in Lipopolysaccharide metabolism.

GO - Molecular functioni

  • mannosyltransferase activity Source: InterPro
  • transferase activity, transferring pentosyl groups Source: EcoCyc

GO - Biological processi

  • lipid A biosynthetic process Source: UniProtKB-HAMAP
  • lipopolysaccharide biosynthetic process Source: EcoCyc
  • protein O-linked glycosylation Source: InterPro
  • response to iron(III) ion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7170-MONOMER.
ECOL316407:JW2251-MONOMER.
MetaCyc:G7170-MONOMER.
BRENDAi2.4.2.43. 2026.
UniPathwayiUPA00037.

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.
TCDBi9.B.142.2.9. the integral membrane glycosyltransferase family 39 (gt39) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (EC:2.4.2.43)
Alternative name(s):
4-amino-4-deoxy-L-arabinose lipid A transferase
Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase
Polymyxin resistance protein PmrK
Undecaprenyl phosphate-alpha-L-Ara4N transferase
Gene namesi
Name:arnT
Synonyms:pmrK, yfbI
Ordered Locus Names:b2257, JW2251
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14093. arnT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Transmembranei110 – 131HelicalSequence analysisAdd BLAST22
Transmembranei137 – 154HelicalSequence analysisAdd BLAST18
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Transmembranei315 – 335HelicalSequence analysisAdd BLAST21
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Transmembranei406 – 426HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215041 – 550Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferaseAdd BLAST550

Proteomic databases

PaxDbiP76473.
PRIDEiP76473.

Expressioni

Inductioni

Induced by BasR.By similarity

Interactioni

Protein-protein interaction databases

BioGridi4260499. 144 interactors.
STRINGi511145.b2257.

Structurei

3D structure databases

ProteinModelPortaliP76473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 83 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105SA9. Bacteria.
COG1807. LUCA.
HOGENOMiHOG000273002.
InParanoidiP76473.
KOiK07264.
OMAiAWRIFRD.
PhylomeDBiP76473.

Family and domain databases

HAMAPiMF_01165. ArnT_transfer. 1 hit.
InterProiIPR022839. ArnT_tfrase.
IPR003342. Glyco_trans_39/83.
[Graphical view]
PfamiPF02366. PMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVRYLIGL FAFIACYYLL PISTRLLWQP DETRYAEISR EMLASGDWIV
60 70 80 90 100
PHLLGLRYFE KPIAGYWINS IGQWLFGANN FGVRAGVIFA TLLTAALVTW
110 120 130 140 150
FTLRLWRDKR LALLATVIYL SLFIVYAIGT YAVLDPFIAF WLVAGMCSFW
160 170 180 190 200
LAMQAQTWKG KSAGFLLLGI TCGMGVMTKG FLALAVPVLS VLPWVATQKR
210 220 230 240 250
WKDLFIYGWL AVISCVLTVL PWGLAIAQRE PNFWHYFFWV EHIQRFALDD
260 270 280 290 300
AQHRAPFWYY VPVIIAGSLP WLGLLPGALY TGWKNRKHSA TVYLLSWTIM
310 320 330 340 350
PLLFFSVAKG KLPTYILSCF ASLAMLMAHY ALLAAKNNPL ALRINGWINI
360 370 380 390 400
AFGVTGIIAT FVVSPWGPMN TPVWQTFESY KVFCAWSIFS LWAFFGWYTL
410 420 430 440 450
TNVEKTWPFA ALCPLGLALL VGFSIPDRVM EGKHPQFFVE MTQESLQPSR
460 470 480 490 500
YILTDSVGVA AGLAWSLQRD DIIMYRQTGE LKYGLNYPDA KGRFVSGDEF
510 520 530 540 550
ANWLNQHRQE GIITLVLSVD RDEDINSLAI PPADAIDRQE RLVLIQYRPK
Length:550
Mass (Da):62,543
Last modified:February 1, 1997 - v1
Checksum:iE57E0C3A0608D745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75317.1.
AP009048 Genomic DNA. Translation: BAE76674.1.
PIRiG64996.
RefSeqiNP_416760.1. NC_000913.3.
WP_000844057.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75317; AAC75317; b2257.
BAE76674; BAE76674; BAE76674.
GeneIDi947297.
KEGGiecj:JW2251.
eco:b2257.
PATRICi32119879. VBIEscCol129921_2349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75317.1.
AP009048 Genomic DNA. Translation: BAE76674.1.
PIRiG64996.
RefSeqiNP_416760.1. NC_000913.3.
WP_000844057.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260499. 144 interactors.
STRINGi511145.b2257.

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.
TCDBi9.B.142.2.9. the integral membrane glycosyltransferase family 39 (gt39) family.

Proteomic databases

PaxDbiP76473.
PRIDEiP76473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75317; AAC75317; b2257.
BAE76674; BAE76674; BAE76674.
GeneIDi947297.
KEGGiecj:JW2251.
eco:b2257.
PATRICi32119879. VBIEscCol129921_2349.

Organism-specific databases

EchoBASEiEB3846.
EcoGeneiEG14093. arnT.

Phylogenomic databases

eggNOGiENOG4105SA9. Bacteria.
COG1807. LUCA.
HOGENOMiHOG000273002.
InParanoidiP76473.
KOiK07264.
OMAiAWRIFRD.
PhylomeDBiP76473.

Enzyme and pathway databases

UniPathwayiUPA00037.
BioCyciEcoCyc:G7170-MONOMER.
ECOL316407:JW2251-MONOMER.
MetaCyc:G7170-MONOMER.
BRENDAi2.4.2.43. 2026.

Miscellaneous databases

PROiP76473.

Family and domain databases

HAMAPiMF_01165. ArnT_transfer. 1 hit.
InterProiIPR022839. ArnT_tfrase.
IPR003342. Glyco_trans_39/83.
[Graphical view]
PfamiPF02366. PMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARNT_ECOLI
AccessioniPrimary (citable) accession number: P76473
Secondary accession number(s): Q2MAN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.