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P76472 (ARND_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD

EC=3.5.1.n3
Gene names
Name:arnD
Synonyms:pmrJ, yfbH
Ordered Locus Names:b2256, JW2250
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the deformylation of 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides Probable. Ref.3

Catalytic activity

4-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + formate. Ref.3

Pathway

Glycolipid biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate: step 2/2. Ref.4

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. Ref.4

Induction

Induced by BasR By similarity. HAMAP-Rule MF_01870

Sequence similarities

Belongs to the polysaccharide deacetylase family. ArnD deformylase subfamily.

Contains 1 NodB homology domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-1128209,EBI-543750

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD HAMAP-Rule MF_01870
PRO_0000169176

Regions

Domain2 – 260259NodB homology

Sequences

Sequence LengthMass (Da)Tools
P76472 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: DDD0BEE55581DE3C

FASTA29633,112
        10         20         30         40         50         60 
MTKVGLRIDV DTFRGTREGV PRLLEILSKH NIQASIFFSV GPDNMGRHLW RLVKPQFLWK 

        70         80         90        100        110        120 
MLRSNAASLY GWDILLAGTA WPGKEIGHAN ADIIREAAKH HEVGLHAWDH HAWQARSGNW 

       130        140        150        160        170        180 
DRQTMIDDIA RGLRTLEEII GQPVTCSAAA GWRADQKVIE AKEAFHLRYN SDCRGAMPFR 

       190        200        210        220        230        240 
PLLESGNPGT AQIPVTLPTW DEVIGRDVKA EDFNGWLLNR ILRDKGTPVY TIHAEVEGCA 

       250        260        270        280        290 
YQHNFVDLLK RAAQEGVTFC PLSELLSETL PLGQVVRGNI AGREGWLGCQ QIAGSR 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-amino-4-deoxy-L-arabinose: identification and function of UDP-4-deoxy-4-formamido-L-arabinose."
Breazeale S.D., Ribeiro A.A., McClerren A.L., Raetz C.R.H.
J. Biol. Chem. 280:14154-14167(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli."
Yan A., Guan Z., Raetz C.R.H.
J. Biol. Chem. 282:36077-36089(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PATHWAY.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC75316.1.
AP009048 Genomic DNA. Translation: BAE76673.1.
PIRF64996.
RefSeqNP_416759.1. NC_000913.3.
YP_490495.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP76472. 1 interaction.
STRING511145.b2256.

Proteomic databases

PaxDbP76472.
PRIDEP76472.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75316; AAC75316; b2256.
BAE76673; BAE76673; BAE76673.
GeneID12931504.
945334.
KEGGecj:Y75_p2219.
eco:b2256.
PATRIC32119877. VBIEscCol129921_2348.

Organism-specific databases

EchoBASEEB3845.
EcoGeneEG14092. arnD.

Phylogenomic databases

eggNOGCOG0726.
HOGENOMHOG000261199.
KOK13014.
OMALHAWDHF.
OrthoDBEOG6423D0.
PhylomeDBP76472.
ProtClustDBPRK15394.

Enzyme and pathway databases

BioCycEcoCyc:G7169-MONOMER.
ECOL316407:JW2250-MONOMER.
UniPathwayUPA00030.
UPA00036; UER00496.

Gene expression databases

GenevestigatorP76472.

Family and domain databases

Gene3D3.20.20.370. 2 hits.
HAMAPMF_01870. ArnD.
InterProIPR023557. ArnD.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. Polysac_deacetylase.
[Graphical view]
PfamPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMSSF88713. SSF88713. 2 hits.
PROSITEPS51677. NODB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP76472.

Entry information

Entry nameARND_ECOLI
AccessionPrimary (citable) accession number: P76472
Secondary accession number(s): Q2MAN3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene