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P76472

- ARND_ECOLI

UniProt

P76472 - ARND_ECOLI

Protein

Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD

Gene

arnD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the deformylation of 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides Probable.1 Publication

    Catalytic activityi

    4-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + formate.1 PublicationUniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process Source: UniProtKB-UniPathway
    2. lipid A biosynthetic process Source: UniProtKB-HAMAP
    3. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
    4. response to antibiotic Source: UniProtKB-KW
    5. response to iron(III) ion Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:G7169-MONOMER.
    ECOL316407:JW2250-MONOMER.
    UniPathwayiUPA00030.
    UPA00036; UER00496.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnDUniRule annotation (EC:3.5.1.n3UniRule annotation)
    Gene namesi
    Name:arnDUniRule annotation
    Synonyms:pmrJ, yfbH
    Ordered Locus Names:b2256, JW2250
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14092. arnD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnDPRO_0000169176Add
    BLAST

    Proteomic databases

    PaxDbiP76472.
    PRIDEiP76472.

    Expressioni

    Inductioni

    Induced by BasR.By similarity

    Gene expression databases

    GenevestigatoriP76472.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    groLP0A6F51EBI-1128209,EBI-543750

    Protein-protein interaction databases

    IntActiP76472. 1 interaction.
    STRINGi511145.b2256.

    Structurei

    3D structure databases

    ProteinModelPortaliP76472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 260259NodB homologyUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polysaccharide deacetylase family. ArnD deformylase subfamily.UniRule annotation
    Contains 1 NodB homology domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0726.
    HOGENOMiHOG000261199.
    KOiK13014.
    OMAiLHAWDHF.
    OrthoDBiEOG6423D0.
    PhylomeDBiP76472.

    Family and domain databases

    Gene3Di3.20.20.370. 2 hits.
    HAMAPiMF_01870. ArnD.
    InterProiIPR023557. ArnD.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR002509. Polysac_deacetylase.
    [Graphical view]
    PfamiPF01522. Polysacc_deac_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF88713. SSF88713. 2 hits.
    PROSITEiPS51677. NODB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P76472-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKVGLRIDV DTFRGTREGV PRLLEILSKH NIQASIFFSV GPDNMGRHLW    50
    RLVKPQFLWK MLRSNAASLY GWDILLAGTA WPGKEIGHAN ADIIREAAKH 100
    HEVGLHAWDH HAWQARSGNW DRQTMIDDIA RGLRTLEEII GQPVTCSAAA 150
    GWRADQKVIE AKEAFHLRYN SDCRGAMPFR PLLESGNPGT AQIPVTLPTW 200
    DEVIGRDVKA EDFNGWLLNR ILRDKGTPVY TIHAEVEGCA YQHNFVDLLK 250
    RAAQEGVTFC PLSELLSETL PLGQVVRGNI AGREGWLGCQ QIAGSR 296
    Length:296
    Mass (Da):33,112
    Last modified:February 1, 1997 - v1
    Checksum:iDDD0BEE55581DE3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75316.1.
    AP009048 Genomic DNA. Translation: BAE76673.1.
    PIRiF64996.
    RefSeqiNP_416759.1. NC_000913.3.
    YP_490495.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75316; AAC75316; b2256.
    BAE76673; BAE76673; BAE76673.
    GeneIDi12931504.
    945334.
    KEGGiecj:Y75_p2219.
    eco:b2256.
    PATRICi32119877. VBIEscCol129921_2348.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75316.1 .
    AP009048 Genomic DNA. Translation: BAE76673.1 .
    PIRi F64996.
    RefSeqi NP_416759.1. NC_000913.3.
    YP_490495.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P76472. 1 interaction.
    STRINGi 511145.b2256.

    Proteomic databases

    PaxDbi P76472.
    PRIDEi P76472.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75316 ; AAC75316 ; b2256 .
    BAE76673 ; BAE76673 ; BAE76673 .
    GeneIDi 12931504.
    945334.
    KEGGi ecj:Y75_p2219.
    eco:b2256.
    PATRICi 32119877. VBIEscCol129921_2348.

    Organism-specific databases

    EchoBASEi EB3845.
    EcoGenei EG14092. arnD.

    Phylogenomic databases

    eggNOGi COG0726.
    HOGENOMi HOG000261199.
    KOi K13014.
    OMAi LHAWDHF.
    OrthoDBi EOG6423D0.
    PhylomeDBi P76472.

    Enzyme and pathway databases

    UniPathwayi UPA00030 .
    UPA00036 ; UER00496 .
    BioCyci EcoCyc:G7169-MONOMER.
    ECOL316407:JW2250-MONOMER.

    Miscellaneous databases

    PROi P76472.

    Gene expression databases

    Genevestigatori P76472.

    Family and domain databases

    Gene3Di 3.20.20.370. 2 hits.
    HAMAPi MF_01870. ArnD.
    InterProi IPR023557. ArnD.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR002509. Polysac_deacetylase.
    [Graphical view ]
    Pfami PF01522. Polysacc_deac_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF88713. SSF88713. 2 hits.
    PROSITEi PS51677. NODB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-amino-4-deoxy-L-arabinose: identification and function of UDP-4-deoxy-4-formamido-L-arabinose."
      Breazeale S.D., Ribeiro A.A., McClerren A.L., Raetz C.R.H.
      J. Biol. Chem. 280:14154-14167(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli."
      Yan A., Guan Z., Raetz C.R.H.
      J. Biol. Chem. 282:36077-36089(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PATHWAY.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiARND_ECOLI
    AccessioniPrimary (citable) accession number: P76472
    Secondary accession number(s): Q2MAN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3