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Protein

Acetyl-CoA acetyltransferase

Gene

atoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 acetyl-CoA = CoA + acetoacetyl-CoA.PROSITE-ProRule annotation

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Acetyl-CoA acetyltransferase (atoB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Acyl-thioester intermediateBy similarity
Active sitei349 – 3491Proton acceptorPROSITE-ProRule annotation
Active sitei379 – 3791Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER.
ECOL316407:JW2218-MONOMER.
MetaCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER.
UniPathwayiUPA00058; UER00101.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-CoA acetyltransferase (EC:2.3.1.9)
Alternative name(s):
Acetoacetyl-CoA thiolase
Gene namesi
Name:atoB
Ordered Locus Names:b2224, JW2218
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11672. atoB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Acetyl-CoA acetyltransferasePRO_0000206406Add
BLAST

Proteomic databases

PaxDbiP76461.
PRIDEiP76461.

Interactioni

Protein-protein interaction databases

BioGridi4263266. 7 interactions.
IntActiP76461. 3 interactions.
STRINGi511145.b2224.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 118Combined sources
Turni20 – 234Combined sources
Helixi26 – 4116Combined sources
Helixi45 – 473Combined sources
Beta strandi50 – 545Combined sources
Helixi65 – 739Combined sources
Beta strandi80 – 856Combined sources
Helixi87 – 893Combined sources
Helixi90 – 10314Combined sources
Beta strandi108 – 11811Combined sources
Beta strandi123 – 1253Combined sources
Helixi128 – 1314Combined sources
Beta strandi138 – 1425Combined sources
Helixi143 – 1486Combined sources
Turni152 – 1543Combined sources
Helixi158 – 16912Combined sources
Helixi173 – 19321Combined sources
Turni194 – 1996Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi211 – 2144Combined sources
Helixi226 – 2305Combined sources
Beta strandi251 – 26111Combined sources
Helixi262 – 2676Combined sources
Beta strandi274 – 28310Combined sources
Helixi286 – 2916Combined sources
Helixi293 – 30311Combined sources
Helixi308 – 3103Combined sources
Beta strandi312 – 3165Combined sources
Helixi321 – 33111Combined sources
Helixi335 – 3373Combined sources
Helixi344 – 3474Combined sources
Helixi351 – 36818Combined sources
Beta strandi373 – 3808Combined sources
Turni381 – 3833Combined sources
Beta strandi384 – 3929Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WYSX-ray2.10A/B/C/D1-393[»]
ProteinModelPortaliP76461.
SMRiP76461. Positions 1-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
HOGENOMiHOG000012240.
InParanoidiP76461.
KOiK00626.
OMAiMGCATIV.
OrthoDBiEOG68M4GV.
PhylomeDBiP76461.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNCVIVSAV RTAIGSFNGS LASTSAIDLG ATVIKAAIER AKIDSQHVDE
60 70 80 90 100
VIMGNVLQAG LGQNPARQAL LKSGLAETVC GFTVNKVCGS GLKSVALAAQ
110 120 130 140 150
AIQAGQAQSI VAGGMENMSL APYLLDAKAR SGYRLGDGQV YDVILRDGLM
160 170 180 190 200
CATHGYHMGI TAENVAKEYG ITREMQDELA LHSQRKAAAA IESGAFTAEI
210 220 230 240 250
VPVNVVTRKK TFVFSQDEFP KANSTAEALG ALRPAFDKAG TVTAGNASGI
260 270 280 290 300
NDGAAALVIM EESAALAAGL TPLARIKSYA SGGVPPALMG MGPVPATQKA
310 320 330 340 350
LQLAGLQLAD IDLIEANEAF AAQFLAVGKN LGFDSEKVNV NGGAIALGHP
360 370 380 390
IGASGARILV TLLHAMQARD KTLGLATLCI GGGQGIAMVI ERLN
Length:394
Mass (Da):40,352
Last modified:February 1, 1997 - v1
Checksum:iDE27C394C3E6BDFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75284.1.
AP009048 Genomic DNA. Translation: BAA16020.1.
PIRiF64992.
RefSeqiNP_416728.1. NC_000913.3.
WP_000786547.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75284; AAC75284; b2224.
BAA16020; BAA16020; BAA16020.
GeneIDi946727.
KEGGiecj:JW2218.
eco:b2224.
PATRICi32119807. VBIEscCol129921_2313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75284.1.
AP009048 Genomic DNA. Translation: BAA16020.1.
PIRiF64992.
RefSeqiNP_416728.1. NC_000913.3.
WP_000786547.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WYSX-ray2.10A/B/C/D1-393[»]
ProteinModelPortaliP76461.
SMRiP76461. Positions 1-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263266. 7 interactions.
IntActiP76461. 3 interactions.
STRINGi511145.b2224.

Proteomic databases

PaxDbiP76461.
PRIDEiP76461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75284; AAC75284; b2224.
BAA16020; BAA16020; BAA16020.
GeneIDi946727.
KEGGiecj:JW2218.
eco:b2224.
PATRICi32119807. VBIEscCol129921_2313.

Organism-specific databases

EchoBASEiEB1623.
EcoGeneiEG11672. atoB.

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
HOGENOMiHOG000012240.
InParanoidiP76461.
KOiK00626.
OMAiMGCATIV.
OrthoDBiEOG68M4GV.
PhylomeDBiP76461.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00101.
BioCyciEcoCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER.
ECOL316407:JW2218-MONOMER.
MetaCyc:ACETYL-COA-ACETYLTRANSFER-MONOMER.

Miscellaneous databases

PROiP76461.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiATOB_ECOLI
AccessioniPrimary (citable) accession number: P76461
Secondary accession number(s): P78176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.