Reviewed, UniProtKB/Swiss-Prot P76459 (ATOA_ECOLI)

Names and origin

Protein names

Recommended name:
    Acetate CoA-transferase subunit beta
    EC=2.8.3.8
Alternative name(s):
    Acetyl-CoA:acetoacetate-CoA transferase subunit beta

Gene names

Name:	atoA
Ordered Locus Names:	b2222, JW2216

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + acetate = a fatty acid anion + acetyl-CoA.
Pathway	Lipid metabolism; short-chain fatty acid metabolism.
Subunit structure	Heterodimer By similarity.
Sequence similarities	Belongs to the 3-oxoacid CoA-transferase subunit B family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetate CoA-transferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yfiO	P0AC02	1	EBI-1128061,EBI-1128087

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Acetate CoA-transferase subunit beta		PRO_0000157915
Sites
	Active site	46	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P76459-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3F3916D024614BAE	FASTA	216	22,960
	10 20 30 40 50 60 MDAKQRIARR VAQELRDGDI VNLGIGLPTM VANYLPEGIH ITLQSENGFL GLGPVTTAHP 70 80 90 100 110 120 DLVNAGGQPC GVLPGAAMFD SAMSFALIRG GHIDACVLGG LQVDEEANLA NWVVPGKMVP 130 140 150 160 170 180 GMGGAMDLVT GSRKVIIAME HCAKDGSAKI LRRCTMPLTA QHAVHMLVTE LAVFRFIDGK 190 200 210 MWLTEIADGC DLATVRAKTE ARFEVAADLN TQRGDL

References

[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC75282.1. AP009048 Genomic DNA. Translation: BAA16018.1.
PIR	D64992.
RefSeq	AP_002821.1. NP_416726.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P76459. 3 interactions.
STRING	P76459.
Proteomic databases
PRIDE	P76459.
Genome annotation databases
GeneID	946719.
GenomeReviews	Gene locus JW2216 in contig AP009048_GR. Gene locus b2222 in contig U00096_GR.
KEGG	ecj:JW2216. eco:b2222.
Organism-specific databases
EchoBASE	EB1621.
EcoGene	EG11670. atoA.
CMR	Search...
Phylogenomic databases
HOGENOM	P76459.
OMA	GGHVDAC
Enzyme and pathway databases
BioCyc	EcoCyc:ATOA-MON. ECOL168927:B2222-MON. MetaCyc:ATOA-MON.
Gene expression databases
Genevestigator	P76459.
Family and domain databases
InterPro	IPR012791. 3-oxoacid_CoA-transf_B. IPR004165. CoA_trans. IPR004164. CoA_transf_AS. [Graphical view]
PANTHER	PTHR13707. CoA_trans. 1 hit.
Pfam	PF01144. CoA_trans. 1 hit. [Graphical view]
SMART	SM00882. CoA_trans. 1 hit. [Graphical view]
TIGRFAMs	TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITE	PS01274. COA_TRANSF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATOA_ECOLI

Accession

Primary (citable) accession number: P76459
Secondary accession number(s): P94762

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)