Reviewed, UniProtKB/Swiss-Prot P76458 (ATOD_ECOLI)

Names and origin

Protein names

Recommended name:
    Acetate CoA-transferase subunit alpha
    EC=2.8.3.8
Alternative name(s):
    Acetyl-CoA:acetoacetate-CoA transferase subunit alpha

Gene names

Name:	atoD
Ordered Locus Names:	b2221, JW2215

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + acetate = a fatty acid anion + acetyl-CoA.
Pathway	Lipid metabolism; short-chain fatty acid metabolism.
Subunit structure	Heterodimer By similarity.
Sequence similarities	Belongs to the 3-oxoacid CoA-transferase subunit A family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetate CoA-transferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-555870,EBI-543750

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 220

220

Acetate CoA-transferase subunit alpha

PRO_0000157904

Regions

Region

24 – 30

7

CoA-binding Potential

Secondary structure

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220

Helix Strand Turn

Details...

Beta strand

4 – 6

3

Helix

8 – 11

4

Helix

12 – 14

3

Beta strand

20 – 23

4

Helix

33 – 42

10

Beta strand

46 – 50

5

Helix

62 – 66

5

Beta strand

70 – 77

8

Helix

82 – 89

8

Beta strand

92 – 97

6

Helix

100 – 111

12

Beta strand

116 – 120

5

Turn

121 – 124

4

Beta strand

133 – 136

4

Beta strand

139 – 144

6

Beta strand

149 – 160

12

Helix

169 – 172

4

Helix

175 – 181

7

Beta strand

182 – 193

12

Helix

200 – 202

3

Helix

207 – 209

3

Beta strand

212 – 214

3

Sequences

	Sequence		Length	Mass (Da)
	P76458-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D1DD69ECEBB2676D	FASTA	220	23,526
	10 20 30 40 50 60 MKTKLMTLQD ATGFFRDGMT IMVGGFMGIG TPSRLVEALL ESGVRDLTLI ANDTAFVDTG 70 80 90 100 110 120 IGPLIVNGRV RKVIASHIGT NPETGRRMIS GEMDVVLVPQ GTLIEQIRCG GAGLGGFLTP 130 140 150 160 170 180 TGVGTVVEEG KQTLTLDGKT WLLERPLRAD LALIRAHRCD TLGNLTYQLS ARNFNPLIAL 190 200 210 220 AADITLVEPD ELVETGELQP DHIVTPGAVI DHIIVSQESK

References

[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U00096 Genomic DNA. Translation: AAC75281.1.
AP009048 Genomic DNA. Translation: BAA16017.1.

PIR

C64992.

RefSeq

AP_002820.1.
NP_416725.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K6D	X-ray	1.90	A/B	1-220	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:9191N.

IntAct

P76458. 2 interactions.

Genome annotation databases

GeneID

947525.

GenomeReviews

Gene locus JW2215 in contig AP009048_GR.
Gene locus b2221 in contig U00096_GR.

KEGG

ecj:JW2215.
eco:b2221.

Organism-specific databases

EchoBASE

EB1620.

EcoGene

EG11669. atoD.

CMR

Search...

Phylogenomic databases

HOGENOM

P76458.

OMA

P76458. VIVEAEN.

Enzyme and pathway databases

BioCyc

EcoCyc:ATOD-MON.
MetaCyc:ATOD-MON.

Family and domain databases

InterPro

IPR012792. 3-oxoacid_CoA-transf_A.
IPR004165. CoA_trans.
IPR004163. CoA_transf_BS.
[Graphical view]

PANTHER

PTHR13707. CoA_trans. 1 hit.

Pfam

PF01144. CoA_trans. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02429. pcaI_scoA_fam. 1 hit.

PROSITE

PS01273. COA_TRANSF_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ATOD_ECOLI

Accession

Primary (citable) accession number: P76458

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)