Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable cyclic di-GMP phosphodiesterase PdeN

Gene

pdeN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG.By similarity

Caution

Was originally (Ref. 4) thought to originate from Proteus vulgaris, but was shown to originate from E.coli.1 Publication

Catalytic activityi

Cyclic di-3',5'-guanylate + H2O = 5'-phosphoguanylyl(3'->5')guanosine.By similarity

GO - Molecular functioni

  • cyclic-guanylate-specific phosphodiesterase activity Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Ligandc-di-GMP

Enzyme and pathway databases

BioCyciEcoCyc:G7148-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cyclic di-GMP phosphodiesterase PdeNCurated (EC:3.1.4.52By similarity)
Gene namesi
Name:pdeN1 Publication
Synonyms:rtn
Ordered Locus Names:b2176, JW2164
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14077 rtn

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975171 – 518Probable cyclic di-GMP phosphodiesterase PdeNAdd BLAST518

Proteomic databases

PaxDbiP76446
PRIDEiP76446

Expressioni

Inductioni

Expressed at both 28 and 37 degrees Celsius during exponential growth. Repressed by RpoS.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261095, 10 interactors
DIPiDIP-10813N
STRINGi316385.ECDH10B_2334

Structurei

3D structure databases

ProteinModelPortaliP76446
SMRiP76446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini261 – 514EALPROSITE-ProRule annotationAdd BLAST254

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CXC Bacteria
COG2200 LUCA
HOGENOMiHOG000273106
InParanoidiP76446
OMAiQLYADSW
PhylomeDBiP76446

Family and domain databases

CDDicd01948 EAL, 1 hit
Gene3Di3.20.20.450, 1 hit
InterProiView protein in InterPro
IPR024744 CSS-motif_dom
IPR001633 EAL_dom
IPR035919 EAL_sf
PfamiView protein in Pfam
PF12792 CSS-motif, 1 hit
PF00563 EAL, 1 hit
SMARTiView protein in SMART
SM00052 EAL, 1 hit
SUPFAMiSSF141868 SSF141868, 1 hit
PROSITEiView protein in PROSITE
PS50883 EAL, 1 hit

Sequencei

Sequence statusi: Complete.

P76446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIRAPNFGR KLLLTCIVAG VMIAILVSCL QFLVAWHKHE VKYDTLITDV
60 70 80 90 100
QKYLDTYFAD LKSTTDRLQP LTLDTCQQAN PELTARAAFS MNVRTFVLVK
110 120 130 140 150
DKKTFCSSAT GEMDIPLNEL IPALDINKNV DMAILPGTPM VPNKPAIVIW
160 170 180 190 200
YRNPLLKNSG VFAALNLNLT PSLFYSSRQE DYDGVALIIG NTALSTFSSR
210 220 230 240 250
LMNVNELTDM PVRETKIAGI PLTVRLYADD WTWNDVWYAF LLGGMSGTVV
260 270 280 290 300
GLLCYYLMSV RMRPGREIMT AIKREQFYVA YQPVVDTQAL RVTGLEVLLR
310 320 330 340 350
WRHPVAGEIP PDAFINFAES QKMIVPLTQH LFELIARDAA ELEKVLPVGV
360 370 380 390 400
KFGINIAPDH LHSESFKADI QKLLTSLPAH HFQIVLEITE RDMLKEQEAT
410 420 430 440 450
QLFAWLHSVG VEIAIDDFGT GHSALIYLER FTLDYLKIDR GFINAIGTET
460 470 480 490 500
ITSPVLDAVL TLAKRLNMLT VAEGVETPEQ ARWLSERGVN FMQGYWISRP
510
LPLDDFVRWL KKPYTPQW
Length:518
Mass (Da):58,588
Last modified:February 1, 1997 - v1
Checksum:iBABA5768CBC47C41
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti482 – 518RWLSE…YTPQW → D no nucleotide entry (Ref. 4) CuratedAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75237.1
AP009048 Genomic DNA Translation: BAA15984.1
U12780 Genomic DNA Translation: AAA50754.1
U83404 Genomic DNA Translation: AAB51508.1
PIRiG64986
RefSeqiNP_416681.1, NC_000913.3
WP_000470560.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75237; AAC75237; b2176
BAA15984; BAA15984; BAA15984
GeneIDi946695
KEGGiecj:JW2164
eco:b2176
PATRICifig|1411691.4.peg.60

Similar proteinsi

Entry informationi

Entry nameiPDEN_ECOLI
AccessioniPrimary (citable) accession number: P76446
Secondary accession number(s): P77139, Q52613
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health