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Protein

Lipid A 1-diphosphate synthase

Gene

lpxT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the modification of the lipid A domain of lipopolysaccharides (LPS). Transfers a phosphate group from undecaprenyl pyrophosphate (C55-PP) to lipid A to form lipid A 1-diphosphate. Contributes to the recycling of undecaprenyl phosphate (C55-P) (PubMed:18047581). In vitro, has low undecaprenyl-diphosphate phosphatase activity (PubMed:17660416).2 Publications

Catalytic activityi

Ditrans-octacis-undecaprenyl diphosphate + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A = ditrans-octacis-undecaprenyl phosphate + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A 1-diphosphate.UniRule annotation1 Publication

Enzyme regulationi

Inhibited by BasR. This regulation does not occur at the level of transcription, but rather following the assembly of LpxT into the inner membrane.1 Publication

pH dependencei

Optimum pH is 7.0. Significant activity is observed from pH 5.5 to pH 7.5.1 Publication

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • phosphotransferase activity, phosphate group as acceptor Source: EcoCyc
  • undecaprenyl-diphosphatase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7146-MONOMER.
ECOL316407:JW2162-MONOMER.
MetaCyc:G7146-MONOMER.
UniPathwayiUPA00030.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A 1-diphosphate synthaseUniRule annotationCurated (EC:2.7.4.29UniRule annotation1 Publication)
Alternative name(s):
Kdo(2)-lipid A phosphotransferaseCurated
Undecaprenyl pyrophosphate:lipid A 1-phosphate phosphotransferaseUniRule annotationCurated
Gene namesi
Name:lpxT1 PublicationUniRule annotation
Synonyms:yeiU
Ordered Locus Names:b2174, JW2162
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14075. lpxT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Cytoplasmic1 Publication
Transmembranei6 – 2621HelicalUniRule annotationAdd
BLAST
Topological domaini27 – 6236Periplasmic1 PublicationAdd
BLAST
Transmembranei63 – 8321HelicalUniRule annotationAdd
BLAST
Topological domaini84 – 907Cytoplasmic1 Publication
Transmembranei91 – 11121HelicalUniRule annotationAdd
BLAST
Topological domaini112 – 14534Periplasmic1 PublicationAdd
BLAST
Topological domaini167 – 1671Cytoplasmic1 Publication
Transmembranei168 – 18821HelicalUniRule annotationAdd
BLAST
Topological domaini189 – 1946Periplasmic1 Publication
Transmembranei195 – 21521HelicalUniRule annotationAdd
BLAST
Topological domaini216 – 23722Cytoplasmic2 PublicationsAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene results in the production of LPS containing only the bis-phosphorylated lipid A species.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi190 – 1901H → A: Lack of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Lipid A 1-diphosphate synthasePRO_0000169160Add
BLAST

Proteomic databases

PaxDbiP76445.

Interactioni

Protein-protein interaction databases

BioGridi4260460. 4 interactions.
STRINGi511145.b2174.

Structurei

3D structure databases

ProteinModelPortaliP76445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxT phosphotransferase family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41076MN. Bacteria.
COG0671. LUCA.
HOGENOMiHOG000282674.
InParanoidiP76445.
KOiK19803.
OMAiRSFPGDH.
PhylomeDBiP76445.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
HAMAPiMF_01945. Lipid_A_LpxT. 1 hit.
InterProiIPR032908. LpxT.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P76445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNLPQIVL LNIVGLALFL SWYIPVNHGF WLPIDADIFY FFNQKLVESK
60 70 80 90 100
AFLWLVALTN NRAFDGCSLL AMGMLMLSFW LKENAPGRRR IVIIGLVMLL
110 120 130 140 150
TAVVLNQLGQ ALIPVKRASP TLTFTDINRV SELLSVPTKD ASRDSFPGDH
160 170 180 190 200
GMMLLIFSAF MWRYFGKVAG LIALIIFVVF AFPRVMIGAH WFTDIIVGSM
210 220 230
TVILIGLPWV LLTPLSDRLI TFFDKSLPGK NKHFQNK
Length:237
Mass (Da):26,759
Last modified:July 15, 1998 - v2
Checksum:iA27F9560A1417D09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75235.2.
AP009048 Genomic DNA. Translation: BAE76645.1.
RefSeqiNP_416679.4. NC_000913.3.
WP_000594599.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75235; AAC75235; b2174.
BAE76645; BAE76645; BAE76645.
GeneIDi946693.
KEGGiecj:JW2162.
eco:b2174.
PATRICi32119703. VBIEscCol129921_2262.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75235.2.
AP009048 Genomic DNA. Translation: BAE76645.1.
RefSeqiNP_416679.4. NC_000913.3.
WP_000594599.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260460. 4 interactions.
STRINGi511145.b2174.

Proteomic databases

PaxDbiP76445.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75235; AAC75235; b2174.
BAE76645; BAE76645; BAE76645.
GeneIDi946693.
KEGGiecj:JW2162.
eco:b2174.
PATRICi32119703. VBIEscCol129921_2262.

Organism-specific databases

EchoBASEiEB3828.
EcoGeneiEG14075. lpxT.

Phylogenomic databases

eggNOGiENOG41076MN. Bacteria.
COG0671. LUCA.
HOGENOMiHOG000282674.
InParanoidiP76445.
KOiK19803.
OMAiRSFPGDH.
PhylomeDBiP76445.

Enzyme and pathway databases

UniPathwayiUPA00030.
BioCyciEcoCyc:G7146-MONOMER.
ECOL316407:JW2162-MONOMER.
MetaCyc:G7146-MONOMER.

Miscellaneous databases

PROiP76445.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
HAMAPiMF_01945. Lipid_A_LpxT. 1 hit.
InterProiIPR032908. LpxT.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXT_ECOLI
AccessioniPrimary (citable) accession number: P76445
Secondary accession number(s): Q2MAR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In E.coli, lipid A contains typically a monophosphate unit at positions 1 and 4' (bis-phosphorylated lipid A). However, one-third of the lipid A contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.