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Protein

Lipid A 1-diphosphate synthase

Gene

lpxT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the modification of the lipid A domain of lipopolysaccharides (LPS). Transfers a phosphate group from undecaprenyl pyrophosphate (C55-PP) to lipid A to form lipid A 1-diphosphate. Contributes to the recycling of undecaprenyl phosphate (C55-P) (PubMed:18047581). In vitro, has low undecaprenyl-diphosphate phosphatase activity (PubMed:17660416).2 Publications

Miscellaneous

In E.coli, lipid A contains typically a monophosphate unit at positions 1 and 4' (bis-phosphorylated lipid A). However, one-third of the lipid A contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate).1 Publication

Catalytic activityi

Ditrans-octacis-undecaprenyl diphosphate + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A = ditrans-octacis-undecaprenyl phosphate + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A 1-diphosphate.UniRule annotation1 Publication

Enzyme regulationi

Inhibited by BasR. This regulation does not occur at the level of transcription, but rather following the assembly of LpxT into the inner membrane.1 Publication

pH dependencei

Optimum pH is 7.0. Significant activity is observed from pH 5.5 to pH 7.5.1 Publication

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • phosphotransferase activity, phosphate group as acceptor Source: EcoCyc
  • undecaprenyl-diphosphatase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7146-MONOMER
MetaCyc:G7146-MONOMER
UniPathwayiUPA00030

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A 1-diphosphate synthaseUniRule annotationCurated (EC:2.7.4.29UniRule annotation1 Publication)
Alternative name(s):
Kdo(2)-lipid A phosphotransferaseCurated
Undecaprenyl pyrophosphate:lipid A 1-phosphate phosphotransferaseUniRule annotationCurated
Gene namesi
Name:lpxT1 PublicationUniRule annotation
Synonyms:yeiU
Ordered Locus Names:b2174, JW2162
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14075 lpxT

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5Cytoplasmic1 Publication5
Transmembranei6 – 26HelicalUniRule annotationAdd BLAST21
Topological domaini27 – 62Periplasmic1 PublicationAdd BLAST36
Transmembranei63 – 83HelicalUniRule annotationAdd BLAST21
Topological domaini84 – 90Cytoplasmic1 Publication7
Transmembranei91 – 111HelicalUniRule annotationAdd BLAST21
Topological domaini112 – 145Periplasmic1 PublicationAdd BLAST34
Topological domaini167Cytoplasmic1 Publication1
Transmembranei168 – 188HelicalUniRule annotationAdd BLAST21
Topological domaini189 – 194Periplasmic1 Publication6
Transmembranei195 – 215HelicalUniRule annotationAdd BLAST21
Topological domaini216 – 237Cytoplasmic2 PublicationsAdd BLAST22

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene results in the production of LPS containing only the bis-phosphorylated lipid A species.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi190H → A: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691601 – 237Lipid A 1-diphosphate synthaseAdd BLAST237

Proteomic databases

PaxDbiP76445
PRIDEiP76445

Interactioni

Protein-protein interaction databases

BioGridi4260460, 4 interactors
STRINGi316385.ECDH10B_2332

Structurei

3D structure databases

ProteinModelPortaliP76445
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxT phosphotransferase family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41076MN Bacteria
COG0671 LUCA
HOGENOMiHOG000282674
InParanoidiP76445
KOiK19803
OMAiRSFPGDH
PhylomeDBiP76445

Family and domain databases

HAMAPiMF_01945 Lipid_A_LpxT, 1 hit
InterProiView protein in InterPro
IPR032908 LpxT
IPR036938 P_Acid_Pase_2/haloperoxi_sf
IPR000326 P_Acid_Pase_2/haloperoxidase
PfamiView protein in Pfam
PF01569 PAP2, 1 hit
SMARTiView protein in SMART
SM00014 acidPPc, 1 hit
SUPFAMiSSF48317 SSF48317, 1 hit

Sequencei

Sequence statusi: Complete.

P76445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNLPQIVL LNIVGLALFL SWYIPVNHGF WLPIDADIFY FFNQKLVESK
60 70 80 90 100
AFLWLVALTN NRAFDGCSLL AMGMLMLSFW LKENAPGRRR IVIIGLVMLL
110 120 130 140 150
TAVVLNQLGQ ALIPVKRASP TLTFTDINRV SELLSVPTKD ASRDSFPGDH
160 170 180 190 200
GMMLLIFSAF MWRYFGKVAG LIALIIFVVF AFPRVMIGAH WFTDIIVGSM
210 220 230
TVILIGLPWV LLTPLSDRLI TFFDKSLPGK NKHFQNK
Length:237
Mass (Da):26,759
Last modified:July 15, 1998 - v2
Checksum:iA27F9560A1417D09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75235.2
AP009048 Genomic DNA Translation: BAE76645.1
RefSeqiNP_416679.4, NC_000913.3
WP_000594599.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75235; AAC75235; b2174
BAE76645; BAE76645; BAE76645
GeneIDi946693
KEGGiecj:JW2162
eco:b2174
PATRICifig|1411691.4.peg.62

Similar proteinsi

Entry informationi

Entry nameiLPXT_ECOLI
AccessioniPrimary (citable) accession number: P76445
Secondary accession number(s): Q2MAR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health