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P76440 (PRET_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD-dependent dihydropyrimidine dehydrogenase subunit PreT

Short name=DPD
EC=1.3.1.1
Alternative name(s):
Dihydrothymine dehydrogenase
Dihydrouracil dehydrogenase
Gene names
Name:preT
Synonyms:yeiT
Ordered Locus Names:b2146, JW2133
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pyrimidine base degradation. Catalyzes physiologically the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate. It also catalyzes the reverse reaction and the reduction of thymine to 5,6-dihydrothymine (DHT). Ref.3 Ref.4

Catalytic activity

5,6-dihydrouracil + NAD+ = uracil + NADH.

5,6-dihydrothymine + NAD+ = thymine + NADH.

Subunit structure

Heterotetramer of 2 PreA and 2 PreT subunits. Ref.4

Induction

Transcriptionally regulated by IscS. Ref.3

Sequence similarities

Belongs to the NADH dehydrogenase family.

Biophysicochemical properties

Kinetic parameters:

KM=38 µM for uracil (at pH 6 and 30 degrees Celsius) Ref.4

KM=87 µM for thymidine (at pH 6 and 30 degrees Celsius)

KM=130 µM for DHT (at pH 11 and 30 degrees Celsius)

KM=160 µM for DHU (at pH 11 and 30 degrees Celsius)

Vmax=0.18 µmol/min/mg enzyme toward DHT (at pH 11 and 30 degrees Celsius)

Vmax=0.26 µmol/min/mg enzyme toward thymidine (at pH 6 and 30 degrees Celsius)

Vmax=0.43 µmol/min/mg enzyme toward uracil (at pH 6 and 30 degrees Celsius)

Vmax=0.44 µmol/min/mg enzyme toward DHU (at pH 11 and 30 degrees Celsius)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412NAD-dependent dihydropyrimidine dehydrogenase subunit PreT
PRO_0000169156

Sites

Binding site2861NAD Probable

Sequences

Sequence LengthMass (Da)Tools
P76440 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 6907F20BFAF7AF4E

FASTA41244,329
        10         20         30         40         50         60 
MPQQNYLDEL TPAFTSLLAI KEASRCLLCH DAPCSQACPA QTDPGKFIRS IYFRNFKGAA 

        70         80         90        100        110        120 
ETIRENNALG AVCARVCPTE KLCQSGCTRA GVDAPIDIGR LQRFVTDFEQ QTGMEIYQPG 

       130        140        150        160        170        180 
TKTLGKVAII GAGPAGLQAS VTLTNQGYDV TIYEKEAHPG GWLRNGIPQF RLPQSVLDAE 

       190        200        210        220        230        240 
IARIEKMGVT IKCNNEVGNT LTLEQLKAEN RAVLVTVGLS SGSGLPLFEH SDVEIAVDFL 

       250        260        270        280        290        300 
QRARQAQGDI SIPQSALIIG GGDVAMDVAS TLKVLGCQAV TCVAREELDE FPASEKEFTS 

       310        320        330        340        350        360 
ARELGVSIID GFTPVAVEGN KVTFKHVRLS GELTMAADKI ILAVGQHARL DAFAELEPQR 

       370        380        390        400        410 
NTIKTQNYQT RDPQVFAAGD IVEGDKTVVY AVKTGKEAAE AIHHYLEGAC SC 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The iscS gene deficiency affects the expression of pyrimidine metabolism genes."
Mihara H., Hidese R., Yamane M., Kurihara T., Esaki N.
Biochem. Biophys. Res. Commun. 372:407-411(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION IN PYRIMIDINE METABOLISM, INDUCTION, NAD.
[4]"Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil."
Hidese R., Mihara H., Kurihara T., Esaki N.
J. Bacteriol. 193:989-993(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A DIHYDROPYRIMIDINE DEHYDROGENASE, BIOPHYSICOCHEMICAL PROPERTIES, NAD BINDING, SUBUNIT.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC75207.1.
AP009048 Genomic DNA. Translation: BAE76623.1.
PIRA64983.
RefSeqNP_416651.1. NC_000913.3.
YP_490385.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76440.
SMRP76440. Positions 19-408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-28055N.
IntActP76440. 5 interactions.
STRING511145.b2146.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75207; AAC75207; b2146.
BAE76623; BAE76623; BAE76623.
GeneID12930175.
949049.
KEGGecj:Y75_p2108.
eco:b2146.
PATRIC32119635. VBIEscCol129921_2228.

Organism-specific databases

EchoBASEEB3827.
EcoGeneEG14074. preT.

Phylogenomic databases

eggNOGCOG0493.
HOGENOMHOG000031439.
KOK17722.
OMAQRNTINT.
OrthoDBEOG6XSZQF.
PhylomeDBP76440.

Enzyme and pathway databases

BioCycEcoCyc:G7145-MONOMER.
ECOL316407:JW2133-MONOMER.
MetaCyc:G7145-MONOMER.

Gene expression databases

GenevestigatorP76440.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR028261. DPD_II.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF14691. Fer4_20. 1 hit.
PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMSSF46548. SSF46548. 1 hit.
ProtoNetSearch...

Other

PROP76440.

Entry information

Entry namePRET_ECOLI
AccessionPrimary (citable) accession number: P76440
Secondary accession number(s): Q2MAT3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene