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P76440

- PRET_ECOLI

UniProt

P76440 - PRET_ECOLI

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Protein

NAD-dependent dihydropyrimidine dehydrogenase subunit PreT

Gene

preT

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pyrimidine base degradation. Catalyzes physiologically the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate. It also catalyzes the reverse reaction and the reduction of thymine to 5,6-dihydrothymine (DHT).2 Publications

Catalytic activityi

5,6-dihydrouracil + NAD+ = uracil + NADH.
5,6-dihydrothymine + NAD+ = thymine + NADH.

Kineticsi

  1. KM=38 µM for uracil (at pH 6 and 30 degrees Celsius)1 Publication
  2. KM=87 µM for thymidine (at pH 6 and 30 degrees Celsius)1 Publication
  3. KM=130 µM for DHT (at pH 11 and 30 degrees Celsius)1 Publication
  4. KM=160 µM for DHU (at pH 11 and 30 degrees Celsius)1 Publication

Vmax=0.18 µmol/min/mg enzyme toward DHT (at pH 11 and 30 degrees Celsius)1 Publication

Vmax=0.26 µmol/min/mg enzyme toward thymidine (at pH 6 and 30 degrees Celsius)1 Publication

Vmax=0.43 µmol/min/mg enzyme toward uracil (at pH 6 and 30 degrees Celsius)1 Publication

Vmax=0.44 µmol/min/mg enzyme toward DHU (at pH 11 and 30 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei286 – 2861NADCurated

GO - Molecular functioni

  1. dihydrouracil dehydrogenase (NAD+) activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. iron-sulfur cluster binding Source: EcoCyc
  4. NADH dehydrogenase activity Source: UniProtKB

GO - Biological processi

  1. metabolic process Source: EcoCyc
  2. pyrimidine nucleobase catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:G7145-MONOMER.
ECOL316407:JW2133-MONOMER.
MetaCyc:G7145-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent dihydropyrimidine dehydrogenase subunit PreT (EC:1.3.1.1)
Short name:
DPD
Alternative name(s):
Dihydrothymine dehydrogenase
Dihydrouracil dehydrogenase
Gene namesi
Name:preT
Synonyms:yeiT
Ordered Locus Names:b2146, JW2133
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14074. preT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412NAD-dependent dihydropyrimidine dehydrogenase subunit PreTPRO_0000169156Add
BLAST

Expressioni

Inductioni

Transcriptionally regulated by IscS.1 Publication

Gene expression databases

GenevestigatoriP76440.

Interactioni

Subunit structurei

Heterotetramer of 2 PreA and 2 PreT subunits.1 Publication

Protein-protein interaction databases

DIPiDIP-28055N.
IntActiP76440. 5 interactions.
STRINGi511145.b2146.

Structurei

3D structure databases

ProteinModelPortaliP76440.
SMRiP76440. Positions 19-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NADH dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG0493.
HOGENOMiHOG000031439.
InParanoidiP76440.
KOiK17722.
OMAiQRNTINT.
OrthoDBiEOG6XSZQF.
PhylomeDBiP76440.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR028261. DPD_II.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF14691. Fer4_20. 1 hit.
PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.

Sequencei

Sequence statusi: Complete.

P76440-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQQNYLDEL TPAFTSLLAI KEASRCLLCH DAPCSQACPA QTDPGKFIRS
60 70 80 90 100
IYFRNFKGAA ETIRENNALG AVCARVCPTE KLCQSGCTRA GVDAPIDIGR
110 120 130 140 150
LQRFVTDFEQ QTGMEIYQPG TKTLGKVAII GAGPAGLQAS VTLTNQGYDV
160 170 180 190 200
TIYEKEAHPG GWLRNGIPQF RLPQSVLDAE IARIEKMGVT IKCNNEVGNT
210 220 230 240 250
LTLEQLKAEN RAVLVTVGLS SGSGLPLFEH SDVEIAVDFL QRARQAQGDI
260 270 280 290 300
SIPQSALIIG GGDVAMDVAS TLKVLGCQAV TCVAREELDE FPASEKEFTS
310 320 330 340 350
ARELGVSIID GFTPVAVEGN KVTFKHVRLS GELTMAADKI ILAVGQHARL
360 370 380 390 400
DAFAELEPQR NTIKTQNYQT RDPQVFAAGD IVEGDKTVVY AVKTGKEAAE
410
AIHHYLEGAC SC
Length:412
Mass (Da):44,329
Last modified:February 1, 1997 - v1
Checksum:i6907F20BFAF7AF4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75207.1.
AP009048 Genomic DNA. Translation: BAE76623.1.
PIRiA64983.
RefSeqiNP_416651.1. NC_000913.3.
YP_490385.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75207; AAC75207; b2146.
BAE76623; BAE76623; BAE76623.
GeneIDi12930175.
949049.
KEGGiecj:Y75_p2108.
eco:b2146.
PATRICi32119635. VBIEscCol129921_2228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75207.1 .
AP009048 Genomic DNA. Translation: BAE76623.1 .
PIRi A64983.
RefSeqi NP_416651.1. NC_000913.3.
YP_490385.1. NC_007779.1.

3D structure databases

ProteinModelPortali P76440.
SMRi P76440. Positions 19-408.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-28055N.
IntActi P76440. 5 interactions.
STRINGi 511145.b2146.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75207 ; AAC75207 ; b2146 .
BAE76623 ; BAE76623 ; BAE76623 .
GeneIDi 12930175.
949049.
KEGGi ecj:Y75_p2108.
eco:b2146.
PATRICi 32119635. VBIEscCol129921_2228.

Organism-specific databases

EchoBASEi EB3827.
EcoGenei EG14074. preT.

Phylogenomic databases

eggNOGi COG0493.
HOGENOMi HOG000031439.
InParanoidi P76440.
KOi K17722.
OMAi QRNTINT.
OrthoDBi EOG6XSZQF.
PhylomeDBi P76440.

Enzyme and pathway databases

BioCyci EcoCyc:G7145-MONOMER.
ECOL316407:JW2133-MONOMER.
MetaCyc:G7145-MONOMER.

Miscellaneous databases

PROi P76440.

Gene expression databases

Genevestigatori P76440.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR028261. DPD_II.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF14691. Fer4_20. 1 hit.
PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view ]
SUPFAMi SSF46548. SSF46548. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The iscS gene deficiency affects the expression of pyrimidine metabolism genes."
    Mihara H., Hidese R., Yamane M., Kurihara T., Esaki N.
    Biochem. Biophys. Res. Commun. 372:407-411(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION IN PYRIMIDINE METABOLISM, INDUCTION, NAD.
  4. "Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil."
    Hidese R., Mihara H., Kurihara T., Esaki N.
    J. Bacteriol. 193:989-993(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DIHYDROPYRIMIDINE DEHYDROGENASE, BIOPHYSICOCHEMICAL PROPERTIES, NAD BINDING, SUBUNIT.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRET_ECOLI
AccessioniPrimary (citable) accession number: P76440
Secondary accession number(s): Q2MAT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3