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Protein

NAD-dependent dihydropyrimidine dehydrogenase subunit PreT

Gene

preT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pyrimidine base degradation. Catalyzes physiologically the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate. It also catalyzes the reverse reaction and the reduction of thymine to 5,6-dihydrothymine (DHT).2 Publications

Catalytic activityi

5,6-dihydrouracil + NAD+ = uracil + NADH.
5,6-dihydrothymine + NAD+ = thymine + NADH.

Kineticsi

  1. KM=38 µM for uracil (at pH 6 and 30 degrees Celsius)1 Publication
  2. KM=87 µM for thymidine (at pH 6 and 30 degrees Celsius)1 Publication
  3. KM=130 µM for DHT (at pH 11 and 30 degrees Celsius)1 Publication
  4. KM=160 µM for DHU (at pH 11 and 30 degrees Celsius)1 Publication
  1. Vmax=0.18 µmol/min/mg enzyme toward DHT (at pH 11 and 30 degrees Celsius)1 Publication
  2. Vmax=0.26 µmol/min/mg enzyme toward thymidine (at pH 6 and 30 degrees Celsius)1 Publication
  3. Vmax=0.43 µmol/min/mg enzyme toward uracil (at pH 6 and 30 degrees Celsius)1 Publication
  4. Vmax=0.44 µmol/min/mg enzyme toward DHU (at pH 11 and 30 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei286NADCurated1

GO - Molecular functioni

  • dihydrouracil dehydrogenase (NAD+) activity Source: UniProtKB-EC
  • iron-sulfur cluster binding Source: EcoCyc
  • NADH dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • pyrimidine nucleobase catabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:G7145-MONOMER
MetaCyc:G7145-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent dihydropyrimidine dehydrogenase subunit PreT (EC:1.3.1.1)
Short name:
DPD
Alternative name(s):
Dihydrothymine dehydrogenase
Dihydrouracil dehydrogenase
Gene namesi
Name:preT
Synonyms:yeiT
Ordered Locus Names:b2146, JW2133
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14074 preT

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691561 – 412NAD-dependent dihydropyrimidine dehydrogenase subunit PreTAdd BLAST412

Proteomic databases

PaxDbiP76440
PRIDEiP76440

Expressioni

Inductioni

Transcriptionally regulated by IscS.1 Publication

Interactioni

Subunit structurei

Heterotetramer of 2 PreA and 2 PreT subunits.1 Publication

Protein-protein interaction databases

BioGridi4259169, 9 interactors
DIPiDIP-28055N
IntActiP76440, 5 interactors
STRINGi316385.ECDH10B_2303

Structurei

3D structure databases

ProteinModelPortaliP76440
SMRiP76440
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NADH dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ8 Bacteria
COG0493 LUCA
HOGENOMiHOG000031439
InParanoidiP76440
KOiK17722
OMAiEQACVRN
PhylomeDBiP76440

Family and domain databases

Gene3Di1.10.1060.10, 1 hit
3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR028261 DPD_II
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR009051 Helical_ferredxn
PfamiView protein in Pfam
PF14691 Fer4_20, 1 hit
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF46548 SSF46548, 1 hit

Sequencei

Sequence statusi: Complete.

P76440-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQQNYLDEL TPAFTSLLAI KEASRCLLCH DAPCSQACPA QTDPGKFIRS
60 70 80 90 100
IYFRNFKGAA ETIRENNALG AVCARVCPTE KLCQSGCTRA GVDAPIDIGR
110 120 130 140 150
LQRFVTDFEQ QTGMEIYQPG TKTLGKVAII GAGPAGLQAS VTLTNQGYDV
160 170 180 190 200
TIYEKEAHPG GWLRNGIPQF RLPQSVLDAE IARIEKMGVT IKCNNEVGNT
210 220 230 240 250
LTLEQLKAEN RAVLVTVGLS SGSGLPLFEH SDVEIAVDFL QRARQAQGDI
260 270 280 290 300
SIPQSALIIG GGDVAMDVAS TLKVLGCQAV TCVAREELDE FPASEKEFTS
310 320 330 340 350
ARELGVSIID GFTPVAVEGN KVTFKHVRLS GELTMAADKI ILAVGQHARL
360 370 380 390 400
DAFAELEPQR NTIKTQNYQT RDPQVFAAGD IVEGDKTVVY AVKTGKEAAE
410
AIHHYLEGAC SC
Length:412
Mass (Da):44,329
Last modified:February 1, 1997 - v1
Checksum:i6907F20BFAF7AF4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75207.1
AP009048 Genomic DNA Translation: BAE76623.1
PIRiA64983
RefSeqiNP_416651.1, NC_000913.3
WP_001136389.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75207; AAC75207; b2146
BAE76623; BAE76623; BAE76623
GeneIDi949049
KEGGiecj:JW2133
eco:b2146
PATRICifig|1411691.4.peg.96

Similar proteinsi

Entry informationi

Entry nameiPRET_ECOLI
AccessioniPrimary (citable) accession number: P76440
Secondary accession number(s): Q2MAT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health