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P76440

- PRET_ECOLI

UniProt

P76440 - PRET_ECOLI

Protein

NAD-dependent dihydropyrimidine dehydrogenase subunit PreT

Gene

preT

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Involved in pyrimidine base degradation. Catalyzes physiologically the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate. It also catalyzes the reverse reaction and the reduction of thymine to 5,6-dihydrothymine (DHT).2 Publications

    Catalytic activityi

    5,6-dihydrouracil + NAD+ = uracil + NADH.
    5,6-dihydrothymine + NAD+ = thymine + NADH.

    Kineticsi

    1. KM=38 µM for uracil (at pH 6 and 30 degrees Celsius)1 Publication
    2. KM=87 µM for thymidine (at pH 6 and 30 degrees Celsius)1 Publication
    3. KM=130 µM for DHT (at pH 11 and 30 degrees Celsius)1 Publication
    4. KM=160 µM for DHU (at pH 11 and 30 degrees Celsius)1 Publication

    Vmax=0.18 µmol/min/mg enzyme toward DHT (at pH 11 and 30 degrees Celsius)1 Publication

    Vmax=0.26 µmol/min/mg enzyme toward thymidine (at pH 6 and 30 degrees Celsius)1 Publication

    Vmax=0.43 µmol/min/mg enzyme toward uracil (at pH 6 and 30 degrees Celsius)1 Publication

    Vmax=0.44 µmol/min/mg enzyme toward DHU (at pH 11 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei286 – 2861NADCurated

    GO - Molecular functioni

    1. dihydrouracil dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. flavin adenine dinucleotide binding Source: InterPro
    3. iron-sulfur cluster binding Source: EcoCyc
    4. NADH dehydrogenase activity Source: UniProtKB
    5. protein binding Source: EcoCyc

    GO - Biological processi

    1. metabolic process Source: EcoCyc
    2. pyrimidine nucleobase catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:G7145-MONOMER.
    ECOL316407:JW2133-MONOMER.
    MetaCyc:G7145-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD-dependent dihydropyrimidine dehydrogenase subunit PreT (EC:1.3.1.1)
    Short name:
    DPD
    Alternative name(s):
    Dihydrothymine dehydrogenase
    Dihydrouracil dehydrogenase
    Gene namesi
    Name:preT
    Synonyms:yeiT
    Ordered Locus Names:b2146, JW2133
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14074. preT.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 412412NAD-dependent dihydropyrimidine dehydrogenase subunit PreTPRO_0000169156Add
    BLAST

    Expressioni

    Inductioni

    Transcriptionally regulated by IscS.1 Publication

    Gene expression databases

    GenevestigatoriP76440.

    Interactioni

    Subunit structurei

    Heterotetramer of 2 PreA and 2 PreT subunits.1 Publication

    Protein-protein interaction databases

    DIPiDIP-28055N.
    IntActiP76440. 5 interactions.
    STRINGi511145.b2146.

    Structurei

    3D structure databases

    ProteinModelPortaliP76440.
    SMRiP76440. Positions 19-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NADH dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG0493.
    HOGENOMiHOG000031439.
    KOiK17722.
    OMAiQRNTINT.
    OrthoDBiEOG6XSZQF.
    PhylomeDBiP76440.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR028261. DPD_II.
    IPR009051. Helical_ferredxn.
    IPR016040. NAD(P)-bd_dom.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF14691. Fer4_20. 1 hit.
    PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF46548. SSF46548. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76440-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQQNYLDEL TPAFTSLLAI KEASRCLLCH DAPCSQACPA QTDPGKFIRS    50
    IYFRNFKGAA ETIRENNALG AVCARVCPTE KLCQSGCTRA GVDAPIDIGR 100
    LQRFVTDFEQ QTGMEIYQPG TKTLGKVAII GAGPAGLQAS VTLTNQGYDV 150
    TIYEKEAHPG GWLRNGIPQF RLPQSVLDAE IARIEKMGVT IKCNNEVGNT 200
    LTLEQLKAEN RAVLVTVGLS SGSGLPLFEH SDVEIAVDFL QRARQAQGDI 250
    SIPQSALIIG GGDVAMDVAS TLKVLGCQAV TCVAREELDE FPASEKEFTS 300
    ARELGVSIID GFTPVAVEGN KVTFKHVRLS GELTMAADKI ILAVGQHARL 350
    DAFAELEPQR NTIKTQNYQT RDPQVFAAGD IVEGDKTVVY AVKTGKEAAE 400
    AIHHYLEGAC SC 412
    Length:412
    Mass (Da):44,329
    Last modified:February 1, 1997 - v1
    Checksum:i6907F20BFAF7AF4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75207.1.
    AP009048 Genomic DNA. Translation: BAE76623.1.
    PIRiA64983.
    RefSeqiNP_416651.1. NC_000913.3.
    YP_490385.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75207; AAC75207; b2146.
    BAE76623; BAE76623; BAE76623.
    GeneIDi12930175.
    949049.
    KEGGiecj:Y75_p2108.
    eco:b2146.
    PATRICi32119635. VBIEscCol129921_2228.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75207.1 .
    AP009048 Genomic DNA. Translation: BAE76623.1 .
    PIRi A64983.
    RefSeqi NP_416651.1. NC_000913.3.
    YP_490385.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76440.
    SMRi P76440. Positions 19-408.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-28055N.
    IntActi P76440. 5 interactions.
    STRINGi 511145.b2146.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75207 ; AAC75207 ; b2146 .
    BAE76623 ; BAE76623 ; BAE76623 .
    GeneIDi 12930175.
    949049.
    KEGGi ecj:Y75_p2108.
    eco:b2146.
    PATRICi 32119635. VBIEscCol129921_2228.

    Organism-specific databases

    EchoBASEi EB3827.
    EcoGenei EG14074. preT.

    Phylogenomic databases

    eggNOGi COG0493.
    HOGENOMi HOG000031439.
    KOi K17722.
    OMAi QRNTINT.
    OrthoDBi EOG6XSZQF.
    PhylomeDBi P76440.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7145-MONOMER.
    ECOL316407:JW2133-MONOMER.
    MetaCyc:G7145-MONOMER.

    Miscellaneous databases

    PROi P76440.

    Gene expression databases

    Genevestigatori P76440.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR028261. DPD_II.
    IPR009051. Helical_ferredxn.
    IPR016040. NAD(P)-bd_dom.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF14691. Fer4_20. 1 hit.
    PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46548. SSF46548. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "The iscS gene deficiency affects the expression of pyrimidine metabolism genes."
      Mihara H., Hidese R., Yamane M., Kurihara T., Esaki N.
      Biochem. Biophys. Res. Commun. 372:407-411(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION IN PYRIMIDINE METABOLISM, INDUCTION, NAD.
    4. "Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil."
      Hidese R., Mihara H., Kurihara T., Esaki N.
      J. Bacteriol. 193:989-993(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A DIHYDROPYRIMIDINE DEHYDROGENASE, BIOPHYSICOCHEMICAL PROPERTIES, NAD BINDING, SUBUNIT.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPRET_ECOLI
    AccessioniPrimary (citable) accession number: P76440
    Secondary accession number(s): Q2MAT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3