Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nickel/cobalt efflux system RcnA

Gene

rcnA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Efflux system for nickel and cobalt.1 Publication

GO - Molecular functioni

  • cation efflux transmembrane transporter activity Source: EcoCyc
  • copper-transporting ATPase activity Source: GO_Central
  • metal ion binding Source: InterPro
  • nickel cation transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cobalt ion transport Source: UniProtKB-KW
  • nickel cation transmembrane transport Source: EcoCyc
  • response to cobalt ion Source: EcoCyc
  • response to nickel cation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Cobalt transport, Ion transport, Nickel transport, Transport

Keywords - Ligandi

Cobalt, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:G7138-MONOMER.
ECOL316407:JW2093-MONOMER.

Protein family/group databases

TCDBi2.A.113.1.1. 2.a.113. the nickel/cobalt transporter (nico) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel/cobalt efflux system RcnA
Gene namesi
Name:rcnA
Synonyms:yohM
Ordered Locus Names:b2106, JW2093
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14071. rcnA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12PeriplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 56CytoplasmicSequence analysisAdd BLAST23
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Topological domaini78 – 86PeriplasmicSequence analysis9
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 175CytoplasmicSequence analysisAdd BLAST68
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 209PeriplasmicSequence analysisAdd BLAST13
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
Topological domaini231 – 251CytoplasmicSequence analysisAdd BLAST21
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Topological domaini273 – 274PeriplasmicSequence analysis2

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940091 – 274Nickel/cobalt efflux system RcnAAdd BLAST274

Proteomic databases

PaxDbiP76425.
PRIDEiP76425.

Expressioni

Inductioni

By nickel and cobalt. Transcriptionally repressed by RcnR. Probably also regulated by Fur. Cadmium, copper and zinc have no effect on the transcription.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260955. 10 interactors.
STRINGi511145.b2106.

Structurei

3D structure databases

ProteinModelPortaliP76425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi121 – 146His-richAdd BLAST26

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41061MD. Bacteria.
COG2215. LUCA.
HOGENOMiHOG000230055.
InParanoidiP76425.
KOiK08970.
OMAiMLWRTWR.
PhylomeDBiP76425.

Family and domain databases

InterProiIPR011541. Ni/Co_transpt_high_affinity.
[Graphical view]
PfamiPF03824. NicO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFTTLLQQ GNAWFFIPSA ILLGALHGLE PGHSKTMMAA FIIAIKGTIK
60 70 80 90 100
QAVMLGLAAT ISHTAVVWLI AFGGMVISKR FTAQSAEPWL QLISAVIIIS
110 120 130 140 150
TAFWMFWRTW RGERNWLENM HGHDYEHHHH DHEHHHDHGH HHHHEHGEYQ
160 170 180 190 200
DAHARAHAND IKRRFDGREV TNWQILLFGL TGGLIPCPAA ITVLLICIQL
210 220 230 240 250
KALTLGATLV VSFSIGLALT LVTVGVGAAI SVQQVAKRWS GFNTLAKRAP
260 270
YFSSLLIGLV GVYMGVHGFM GIMR
Length:274
Mass (Da):30,419
Last modified:February 1, 1997 - v1
Checksum:i82C99F416E254C59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75167.1.
AP009048 Genomic DNA. Translation: BAA15973.1.
PIRiA64978.
RefSeqiNP_416609.1. NC_000913.3.
WP_000134636.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75167; AAC75167; b2106.
BAA15973; BAA15973; BAA15973.
GeneIDi949078.
KEGGiecj:JW2093.
eco:b2106.
PATRICi32119545. VBIEscCol129921_2183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75167.1.
AP009048 Genomic DNA. Translation: BAA15973.1.
PIRiA64978.
RefSeqiNP_416609.1. NC_000913.3.
WP_000134636.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260955. 10 interactors.
STRINGi511145.b2106.

Protein family/group databases

TCDBi2.A.113.1.1. 2.a.113. the nickel/cobalt transporter (nico) family.

Proteomic databases

PaxDbiP76425.
PRIDEiP76425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75167; AAC75167; b2106.
BAA15973; BAA15973; BAA15973.
GeneIDi949078.
KEGGiecj:JW2093.
eco:b2106.
PATRICi32119545. VBIEscCol129921_2183.

Organism-specific databases

EchoBASEiEB3824.
EcoGeneiEG14071. rcnA.

Phylogenomic databases

eggNOGiENOG41061MD. Bacteria.
COG2215. LUCA.
HOGENOMiHOG000230055.
InParanoidiP76425.
KOiK08970.
OMAiMLWRTWR.
PhylomeDBiP76425.

Enzyme and pathway databases

BioCyciEcoCyc:G7138-MONOMER.
ECOL316407:JW2093-MONOMER.

Miscellaneous databases

PROiP76425.

Family and domain databases

InterProiIPR011541. Ni/Co_transpt_high_affinity.
[Graphical view]
PfamiPF03824. NicO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCNA_ECOLI
AccessioniPrimary (citable) accession number: P76425
Secondary accession number(s): O08015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.