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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation1 Publication

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Substrate; via amide nitrogenUniRule annotation1
Binding sitei125ATPUniRule annotation1
Binding sitei171ATPUniRule annotation1
Binding sitei198Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydroxyethylthiazole kinase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  • thiamine salvage Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THZ-KIN-MONOMER.
MetaCyc:THZ-KIN-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:b2104, JW2091
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14069. thiM.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569331 – 262Hydroxyethylthiazole kinaseAdd BLAST262

Proteomic databases

PaxDbiP76423.
PRIDEiP76423.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261165. 302 interactors.
IntActiP76423. 2 interactors.
STRINGi316385.ECDH10B_2257.

Structurei

3D structure databases

ProteinModelPortaliP76423.
SMRiP76423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
HOGENOMiHOG000114352.
InParanoidiP76423.
KOiK00878.
PhylomeDBiP76423.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiView protein in InterPro
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
PfamiView protein in Pfam
PF02110. HK. 1 hit.
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P76423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP
110 120 130 140 150
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA
160 170 180 190 200
ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG
210 220 230 240 250
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL
260
DALWQLTQEV QA
Length:262
Mass (Da):27,339
Last modified:February 1, 1997 - v1
Checksum:i720774031BA7AC4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1.
AP009048 Genomic DNA. Translation: BAA15972.1.
D88442 Genomic DNA. Translation: BAA76743.1.
PIRiG64977.
RefSeqiNP_416607.1. NC_000913.3.
WP_001195564.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75165; AAC75165; b2104.
BAA15972; BAA15972; BAA15972.
GeneIDi945142.
KEGGiecj:JW2091.
eco:b2104.
PATRICifig|1411691.4.peg.143.

Similar proteinsi

Entry informationi

Entry nameiTHIM_ECOLI
AccessioniPrimary (citable) accession number: P76423
Secondary accession number(s): Q9S6P4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: August 30, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families