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P76423

- THIM_ECOLI

UniProt

P76423 - THIM_ECOLI

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Protein
Hydroxyethylthiazole kinase
Gene
thiM, b2104, JW2091
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogen By similarity
Binding sitei125 – 1251ATP By similarity
Binding sitei171 – 1711ATP By similarity
Binding sitei198 – 1981Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: EcoCyc
  3. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
Thz kinase
Gene namesi
Name:thiM
Ordered Locus Names:b2104, JW2091
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14069. thiM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Hydroxyethylthiazole kinaseUniRule annotation
PRO_0000156933Add
BLAST

Proteomic databases

PaxDbiP76423.
PRIDEiP76423.

Expressioni

Gene expression databases

GenevestigatoriP76423.

Interactioni

Protein-protein interaction databases

IntActiP76423. 2 interactions.
STRINGi511145.b2104.

Structurei

3D structure databases

ProteinModelPortaliP76423.
SMRiP76423. Positions 6-259.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiQTFTANV.
OrthoDBiEOG628F8M.
PhylomeDBiP76423.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P76423-1 [UniParc]FASTAAdd to Basket

« Hide

MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM    50
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP 100
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA 150
ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG 200
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL 250
DALWQLTQEV QA 262
Length:262
Mass (Da):27,339
Last modified:February 1, 1997 - v1
Checksum:i720774031BA7AC4B
GO

Sequence cautioni

The sequence BAA76743.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1.
AP009048 Genomic DNA. Translation: BAA15972.1.
D88442 Genomic DNA. Translation: BAA76743.1. Different initiation.
PIRiG64977.
RefSeqiNP_416607.1. NC_000913.3.
YP_490344.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75165; AAC75165; b2104.
BAA15972; BAA15972; BAA15972.
GeneIDi12932171.
945142.
KEGGiecj:Y75_p2066.
eco:b2104.
PATRICi32119541. VBIEscCol129921_2181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1 .
AP009048 Genomic DNA. Translation: BAA15972.1 .
D88442 Genomic DNA. Translation: BAA76743.1 . Different initiation.
PIRi G64977.
RefSeqi NP_416607.1. NC_000913.3.
YP_490344.1. NC_007779.1.

3D structure databases

ProteinModelPortali P76423.
SMRi P76423. Positions 6-259.
ModBasei Search...

Protein-protein interaction databases

IntActi P76423. 2 interactions.
STRINGi 511145.b2104.

Proteomic databases

PaxDbi P76423.
PRIDEi P76423.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75165 ; AAC75165 ; b2104 .
BAA15972 ; BAA15972 ; BAA15972 .
GeneIDi 12932171.
945142.
KEGGi ecj:Y75_p2066.
eco:b2104.
PATRICi 32119541. VBIEscCol129921_2181.

Organism-specific databases

EchoBASEi EB3822.
EcoGenei EG14069. thiM.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
KOi K00878.
OMAi QTFTANV.
OrthoDBi EOG628F8M.
PhylomeDBi P76423.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci EcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.

Miscellaneous databases

PROi P76423.

Gene expression databases

Genevestigatori P76423.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
    Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
    Microbiology 145:495-501(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-262.
    Strain: K12.

Entry informationi

Entry nameiTHIM_ECOLI
AccessioniPrimary (citable) accession number: P76423
Secondary accession number(s): Q9S6P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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