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P76423 (THIM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:b2104, JW2091
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence caution

The sequence BAA76743.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000156933

Sites

Binding site501Substrate; via amide nitrogen By similarity
Binding site1251ATP By similarity
Binding site1711ATP By similarity
Binding site1981Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P76423 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 720774031BA7AC4B

FASTA26227,339
        10         20         30         40         50         60 
MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASQF 

        70         80         90        100        110        120 
AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP VAVGALDYRR HFCHELLSFK 

       130        140        150        160        170        180 
PAAIRGNASE IMALAGIANG GRGVDTTDAA ANAIPAAQTL ARETGAIVVV TGEMDYVTDG 

       190        200        210        220        230        240 
HRIIGIHGGD PLMTKVVGTG CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE 

       250        260 
GPGSFVPHFL DALWQLTQEV QA 

« Hide

References

« Hide 'large scale' references
[1]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
Microbiology 145:495-501(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-262.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC75165.1.
AP009048 Genomic DNA. Translation: BAA15972.1.
D88442 Genomic DNA. Translation: BAA76743.1. Different initiation.
PIRG64977.
RefSeqNP_416607.1. NC_000913.3.
YP_490344.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76423.
SMRP76423. Positions 6-259.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP76423. 2 interactions.
STRING511145.b2104.

Proteomic databases

PaxDbP76423.
PRIDEP76423.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75165; AAC75165; b2104.
BAA15972; BAA15972; BAA15972.
GeneID12932171.
945142.
KEGGecj:Y75_p2066.
eco:b2104.
PATRIC32119541. VBIEscCol129921_2181.

Organism-specific databases

EchoBASEEB3822.
EcoGeneEG14069. thiM.

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAQTFTANV.
OrthoDBEOG628F8M.
PhylomeDBP76423.

Enzyme and pathway databases

BioCycEcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.
UniPathwayUPA00060; UER00139.

Gene expression databases

GenevestigatorP76423.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Other

PROP76423.

Entry information

Entry nameTHIM_ECOLI
AccessionPrimary (citable) accession number: P76423
Secondary accession number(s): Q9S6P4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene