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P76423

- THIM_ECOLI

UniProt

P76423 - THIM_ECOLI

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).1 PublicationUniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.1 PublicationUniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
Binding sitei125 – 1251ATPUniRule annotation
Binding sitei171 – 1711ATPUniRule annotation
Binding sitei198 – 1981Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: EcoCyc
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:b2104, JW2091
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14069. thiM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Hydroxyethylthiazole kinasePRO_0000156933Add
BLAST

Proteomic databases

PaxDbiP76423.
PRIDEiP76423.

Expressioni

Gene expression databases

GenevestigatoriP76423.

Interactioni

Protein-protein interaction databases

IntActiP76423. 2 interactions.
STRINGi511145.b2104.

Structurei

3D structure databases

ProteinModelPortaliP76423.
SMRiP76423. Positions 6-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
InParanoidiP76423.
KOiK00878.
OMAiQTFTANV.
OrthoDBiEOG628F8M.
PhylomeDBiP76423.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P76423-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP
110 120 130 140 150
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA
160 170 180 190 200
ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG
210 220 230 240 250
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL
260
DALWQLTQEV QA
Length:262
Mass (Da):27,339
Last modified:February 1, 1997 - v1
Checksum:i720774031BA7AC4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1.
AP009048 Genomic DNA. Translation: BAA15972.1.
D88442 Genomic DNA. Translation: BAA76743.1.
PIRiG64977.
RefSeqiNP_416607.1. NC_000913.3.
YP_490344.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75165; AAC75165; b2104.
BAA15972; BAA15972; BAA15972.
GeneIDi12932171.
945142.
KEGGiecj:Y75_p2066.
eco:b2104.
PATRICi32119541. VBIEscCol129921_2181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1 .
AP009048 Genomic DNA. Translation: BAA15972.1 .
D88442 Genomic DNA. Translation: BAA76743.1 .
PIRi G64977.
RefSeqi NP_416607.1. NC_000913.3.
YP_490344.1. NC_007779.1.

3D structure databases

ProteinModelPortali P76423.
SMRi P76423. Positions 6-259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P76423. 2 interactions.
STRINGi 511145.b2104.

Proteomic databases

PaxDbi P76423.
PRIDEi P76423.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75165 ; AAC75165 ; b2104 .
BAA15972 ; BAA15972 ; BAA15972 .
GeneIDi 12932171.
945142.
KEGGi ecj:Y75_p2066.
eco:b2104.
PATRICi 32119541. VBIEscCol129921_2181.

Organism-specific databases

EchoBASEi EB3822.
EcoGenei EG14069. thiM.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
InParanoidi P76423.
KOi K00878.
OMAi QTFTANV.
OrthoDBi EOG628F8M.
PhylomeDBi P76423.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci EcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.

Miscellaneous databases

PROi P76423.

Gene expression databases

Genevestigatori P76423.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
    Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
    Microbiology 145:495-501(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-262.
    Strain: K12.
  5. "The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli."
    Mizote T., Nakayama H.
    J. Bacteriol. 171:3228-3232(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, GENE NAME.
    Strain: K12.

Entry informationi

Entry nameiTHIM_ECOLI
AccessioniPrimary (citable) accession number: P76423
Secondary accession number(s): Q9S6P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3