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P76423

- THIM_ECOLI

UniProt

P76423 - THIM_ECOLI

Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).1 PublicationUniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
    Binding sitei125 – 1251ATPUniRule annotation
    Binding sitei171 – 1711ATPUniRule annotation
    Binding sitei198 – 1981Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: EcoCyc
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:THZ-KIN-MONOMER.
    ECOL316407:JW2091-MONOMER.
    MetaCyc:THZ-KIN-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
    Short name:
    TH kinaseUniRule annotation
    Short name:
    Thz kinaseUniRule annotation
    Gene namesi
    Name:thiMUniRule annotation
    Ordered Locus Names:b2104, JW2091
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14069. thiM.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Hydroxyethylthiazole kinasePRO_0000156933Add
    BLAST

    Proteomic databases

    PaxDbiP76423.
    PRIDEiP76423.

    Expressioni

    Gene expression databases

    GenevestigatoriP76423.

    Interactioni

    Protein-protein interaction databases

    IntActiP76423. 2 interactions.
    STRINGi511145.b2104.

    Structurei

    3D structure databases

    ProteinModelPortaliP76423.
    SMRiP76423. Positions 6-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    HOGENOMiHOG000114352.
    KOiK00878.
    OMAiQTFTANV.
    OrthoDBiEOG628F8M.
    PhylomeDBiP76423.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00694. thiM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76423-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM    50
    VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP 100
    VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA 150
    ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG 200
    CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL 250
    DALWQLTQEV QA 262
    Length:262
    Mass (Da):27,339
    Last modified:February 1, 1997 - v1
    Checksum:i720774031BA7AC4B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75165.1.
    AP009048 Genomic DNA. Translation: BAA15972.1.
    D88442 Genomic DNA. Translation: BAA76743.1.
    PIRiG64977.
    RefSeqiNP_416607.1. NC_000913.3.
    YP_490344.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75165; AAC75165; b2104.
    BAA15972; BAA15972; BAA15972.
    GeneIDi12932171.
    945142.
    KEGGiecj:Y75_p2066.
    eco:b2104.
    PATRICi32119541. VBIEscCol129921_2181.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC75165.1 .
    AP009048 Genomic DNA. Translation: BAA15972.1 .
    D88442 Genomic DNA. Translation: BAA76743.1 .
    PIRi G64977.
    RefSeqi NP_416607.1. NC_000913.3.
    YP_490344.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76423.
    SMRi P76423. Positions 6-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P76423. 2 interactions.
    STRINGi 511145.b2104.

    Proteomic databases

    PaxDbi P76423.
    PRIDEi P76423.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75165 ; AAC75165 ; b2104 .
    BAA15972 ; BAA15972 ; BAA15972 .
    GeneIDi 12932171.
    945142.
    KEGGi ecj:Y75_p2066.
    eco:b2104.
    PATRICi 32119541. VBIEscCol129921_2181.

    Organism-specific databases

    EchoBASEi EB3822.
    EcoGenei EG14069. thiM.

    Phylogenomic databases

    eggNOGi COG2145.
    HOGENOMi HOG000114352.
    KOi K00878.
    OMAi QTFTANV.
    OrthoDBi EOG628F8M.
    PhylomeDBi P76423.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci EcoCyc:THZ-KIN-MONOMER.
    ECOL316407:JW2091-MONOMER.
    MetaCyc:THZ-KIN-MONOMER.

    Miscellaneous databases

    PROi P76423.

    Gene expression databases

    Genevestigatori P76423.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00694. thiM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
      Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
      Microbiology 145:495-501(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-262.
      Strain: K12.
    5. "The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli."
      Mizote T., Nakayama H.
      J. Bacteriol. 171:3228-3232(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, GENE NAME.
      Strain: K12.

    Entry informationi

    Entry nameiTHIM_ECOLI
    AccessioniPrimary (citable) accession number: P76423
    Secondary accession number(s): Q9S6P4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3