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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation1 Publication

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Pathway:ithiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
Binding sitei125 – 1251ATPUniRule annotation
Binding sitei171 – 1711ATPUniRule annotation
Binding sitei198 – 1981Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • hydroxyethylthiazole kinase activity Source: EcoCyc
  • magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:b2104, JW2091
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14069. thiM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Hydroxyethylthiazole kinasePRO_0000156933Add
BLAST

Proteomic databases

PaxDbiP76423.
PRIDEiP76423.

Interactioni

Protein-protein interaction databases

IntActiP76423. 2 interactions.
STRINGi511145.b2104.

Structurei

3D structure databases

ProteinModelPortaliP76423.
SMRiP76423. Positions 6-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
InParanoidiP76423.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.
PhylomeDBiP76423.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P76423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP
110 120 130 140 150
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA
160 170 180 190 200
ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG
210 220 230 240 250
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL
260
DALWQLTQEV QA
Length:262
Mass (Da):27,339
Last modified:February 1, 1997 - v1
Checksum:i720774031BA7AC4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1.
AP009048 Genomic DNA. Translation: BAA15972.1.
D88442 Genomic DNA. Translation: BAA76743.1.
PIRiG64977.
RefSeqiNP_416607.1. NC_000913.3.
WP_001195564.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC75165; AAC75165; b2104.
BAA15972; BAA15972; BAA15972.
GeneIDi945142.
KEGGieco:b2104.
PATRICi32119541. VBIEscCol129921_2181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75165.1.
AP009048 Genomic DNA. Translation: BAA15972.1.
D88442 Genomic DNA. Translation: BAA76743.1.
PIRiG64977.
RefSeqiNP_416607.1. NC_000913.3.
WP_001195564.1. NZ_CP010445.1.

3D structure databases

ProteinModelPortaliP76423.
SMRiP76423. Positions 6-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP76423. 2 interactions.
STRINGi511145.b2104.

Proteomic databases

PaxDbiP76423.
PRIDEiP76423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75165; AAC75165; b2104.
BAA15972; BAA15972; BAA15972.
GeneIDi945142.
KEGGieco:b2104.
PATRICi32119541. VBIEscCol129921_2181.

Organism-specific databases

EchoBASEiEB3822.
EcoGeneiEG14069. thiM.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
InParanoidiP76423.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.
PhylomeDBiP76423.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciEcoCyc:THZ-KIN-MONOMER.
ECOL316407:JW2091-MONOMER.
MetaCyc:THZ-KIN-MONOMER.

Miscellaneous databases

PROiP76423.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
    Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
    Microbiology 145:495-501(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-262.
    Strain: K12.
  5. "The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli."
    Mizote T., Nakayama H.
    J. Bacteriol. 171:3228-3232(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, GENE NAME.
    Strain: K12.

Entry informationi

Entry nameiTHIM_ECOLI
AccessioniPrimary (citable) accession number: P76423
Secondary accession number(s): Q9S6P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 22, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.