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Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene

thiD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine.1 Publication

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-2-methyl-5-(phosphomethyl)pyrimidine.1 Publication
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine.1 Publication

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD)
  3. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • phosphorylation Source: EcoCyc
  • thiamine biosynthetic process Source: EcoCyc
  • thiamine diphosphate biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HMP-P-KIN-MONOMER
MetaCyc:HMP-P-KIN-MONOMER
BRENDAi2.7.1.49 2026
UniPathwayiUPA00060; UER00137
UPA00060; UER00138

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.491 Publication, EC:2.7.4.71 Publication)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:thiD
Synonyms:thiJ
Ordered Locus Names:b2103, JW2090
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14068 thiD

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001920181 – 266Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseAdd BLAST266

Proteomic databases

EPDiP76422
PaxDbiP76422
PRIDEiP76422

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi42615368 interactors.
DIPiDIP-6867N
IntActiP76422 7 interactors.
STRINGi316385.ECDH10B_2256

Structurei

3D structure databases

ProteinModelPortaliP76422
SMRiP76422
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ThiD family.Curated

Phylogenomic databases

eggNOGiENOG4105DWF Bacteria
COG0351 LUCA
HOGENOMiHOG000225275
InParanoidiP76422
KOiK00941
OMAiVKIGMLH
PhylomeDBiP76422

Family and domain databases

CDDicd01169 HMPP_kinase, 1 hit
Gene3Di3.40.1190.201 hit
InterProiView protein in InterPro
IPR004399 HMP/HMP-P_kinase
IPR013749 PM/HMP-P_kinase-1
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF08543 Phos_pyr_kin, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00097 HMP-P_kinase, 1 hit

Sequencei

Sequence statusi: Complete.

P76422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRINALTIA GTDPSGGAGI QADLKTFSAL GAYGCSVITA LVAQNTRGVQ
60 70 80 90 100
SVYRIEPDFV AAQLDSVFSD VRIDTTKIGM LAETDIVEAV AERLQRYQIQ
110 120 130 140 150
NVVLDTVMLA KSGDPLLSPS AVATLRSRLL PQVSLITPNL PEAAALLDAP
160 170 180 190 200
HARTEQEMLE QGRSLLAMGC GAVLMKGGHL DDEQSPDWLF TREGEQRFTA
210 220 230 240 250
PRIMTKNTHG TGCTLSAALA ALRPRHTNWA DTVQEAKSWL SSALAQADTL
260
EVGHGIGPVH HFHAWW
Length:266
Mass (Da):28,634
Last modified:February 1, 1997 - v1
Checksum:iE555CEFE445B8F0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84200 Genomic DNA Translation: BAA76742.1
U00096 Genomic DNA Translation: AAC75164.1
AP009048 Genomic DNA Translation: BAA15971.1
PIRiF64977
RefSeqiNP_416606.1, NC_000913.3
WP_000822274.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75164; AAC75164; b2103
BAA15971; BAA15971; BAA15971
GeneIDi946459
KEGGiecj:JW2090
eco:b2103
PATRICifig|1411691.4.peg.144

Similar proteinsi

Entry informationi

Entry nameiTHID_ECOLI
AccessioniPrimary (citable) accession number: P76422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome