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Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene

thiD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine.

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.1 Publication
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: EcoCyc
  3. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. phosphorylation Source: EcoCyc
  2. thiamine biosynthetic process Source: EcoCyc
  3. thiamine diphosphate biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HMP-P-KIN-MONOMER.
ECOL316407:JW2090-MONOMER.
MetaCyc:HMP-P-KIN-MONOMER.
BRENDAi2.7.1.49. 2026.
UniPathwayiUPA00060; UER00137.
UPA00060; UER00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:thiD
Synonyms:thiJ
Ordered Locus Names:b2103, JW2090
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14068. thiD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Hydroxymethylpyrimidine/phosphomethylpyrimidine kinasePRO_0000192018Add
BLAST

Proteomic databases

PaxDbiP76422.
PRIDEiP76422.

Expressioni

Gene expression databases

GenevestigatoriP76422.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

DIPiDIP-6867N.
IntActiP76422. 7 interactions.
STRINGi511145.b2103.

Structurei

3D structure databases

ProteinModelPortaliP76422.
SMRiP76422. Positions 1-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ThiD family.Curated

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000225275.
InParanoidiP76422.
KOiK00941.
OMAiGPLNHWA.
OrthoDBiEOG6XWV53.
PhylomeDBiP76422.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.

Sequencei

Sequence statusi: Complete.

P76422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRINALTIA GTDPSGGAGI QADLKTFSAL GAYGCSVITA LVAQNTRGVQ
60 70 80 90 100
SVYRIEPDFV AAQLDSVFSD VRIDTTKIGM LAETDIVEAV AERLQRYQIQ
110 120 130 140 150
NVVLDTVMLA KSGDPLLSPS AVATLRSRLL PQVSLITPNL PEAAALLDAP
160 170 180 190 200
HARTEQEMLE QGRSLLAMGC GAVLMKGGHL DDEQSPDWLF TREGEQRFTA
210 220 230 240 250
PRIMTKNTHG TGCTLSAALA ALRPRHTNWA DTVQEAKSWL SSALAQADTL
260
EVGHGIGPVH HFHAWW
Length:266
Mass (Da):28,634
Last modified:January 31, 1997 - v1
Checksum:iE555CEFE445B8F0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84200 Genomic DNA. Translation: BAA76742.1.
U00096 Genomic DNA. Translation: AAC75164.1.
AP009048 Genomic DNA. Translation: BAA15971.1.
PIRiF64977.
RefSeqiNP_416606.1. NC_000913.3.
YP_490343.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75164; AAC75164; b2103.
BAA15971; BAA15971; BAA15971.
GeneIDi12932542.
946459.
KEGGiecj:Y75_p2065.
eco:b2103.
PATRICi32119539. VBIEscCol129921_2180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84200 Genomic DNA. Translation: BAA76742.1.
U00096 Genomic DNA. Translation: AAC75164.1.
AP009048 Genomic DNA. Translation: BAA15971.1.
PIRiF64977.
RefSeqiNP_416606.1. NC_000913.3.
YP_490343.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP76422.
SMRiP76422. Positions 1-266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6867N.
IntActiP76422. 7 interactions.
STRINGi511145.b2103.

Proteomic databases

PaxDbiP76422.
PRIDEiP76422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75164; AAC75164; b2103.
BAA15971; BAA15971; BAA15971.
GeneIDi12932542.
946459.
KEGGiecj:Y75_p2065.
eco:b2103.
PATRICi32119539. VBIEscCol129921_2180.

Organism-specific databases

EchoBASEiEB3821.
EcoGeneiEG14068. thiD.

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000225275.
InParanoidiP76422.
KOiK00941.
OMAiGPLNHWA.
OrthoDBiEOG6XWV53.
PhylomeDBiP76422.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00137.
UPA00060; UER00138.
BioCyciEcoCyc:HMP-P-KIN-MONOMER.
ECOL316407:JW2090-MONOMER.
MetaCyc:HMP-P-KIN-MONOMER.
BRENDAi2.7.1.49. 2026.

Miscellaneous databases

PROiP76422.

Gene expression databases

GenevestigatoriP76422.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
    Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
    Microbiology 145:495-501(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiTHID_ECOLI
AccessioniPrimary (citable) accession number: P76422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 31, 1997
Last modified: March 31, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.