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P76422

- THID_ECOLI

UniProt

P76422 - THID_ECOLI

Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene

thiD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine.

    Catalytic activityi

    ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.1 Publication
    ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydroxymethylpyrimidine kinase activity Source: EcoCyc
    3. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. phosphorylation Source: EcoCyc
    2. thiamine biosynthetic process Source: EcoCyc
    3. thiamine diphosphate biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:HMP-P-KIN-MONOMER.
    ECOL316407:JW2090-MONOMER.
    MetaCyc:HMP-P-KIN-MONOMER.
    UniPathwayiUPA00060; UER00137.
    UPA00060; UER00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
    Alternative name(s):
    Hydroxymethylpyrimidine kinase
    Short name:
    HMP kinase
    Hydroxymethylpyrimidine phosphate kinase
    Short name:
    HMP-P kinase
    Short name:
    HMP-phosphate kinase
    Short name:
    HMPP kinase
    Gene namesi
    Name:thiD
    Synonyms:thiJ
    Ordered Locus Names:b2103, JW2090
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14068. thiD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Hydroxymethylpyrimidine/phosphomethylpyrimidine kinasePRO_0000192018Add
    BLAST

    Proteomic databases

    PaxDbiP76422.
    PRIDEiP76422.

    Expressioni

    Gene expression databases

    GenevestigatoriP76422.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-6867N.
    IntActiP76422. 7 interactions.
    STRINGi511145.b2103.

    Structurei

    3D structure databases

    ProteinModelPortaliP76422.
    SMRiP76422. Positions 1-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ThiD family.Curated

    Phylogenomic databases

    eggNOGiCOG0351.
    HOGENOMiHOG000225275.
    KOiK00941.
    OMAiTNHWAYS.
    OrthoDBiEOG6XWV53.
    PhylomeDBiP76422.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    InterProiIPR004399. HMP/HMP-P_kinase.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF08543. Phos_pyr_kin. 1 hit.
    [Graphical view]
    SUPFAMiSSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRINALTIA GTDPSGGAGI QADLKTFSAL GAYGCSVITA LVAQNTRGVQ    50
    SVYRIEPDFV AAQLDSVFSD VRIDTTKIGM LAETDIVEAV AERLQRYQIQ 100
    NVVLDTVMLA KSGDPLLSPS AVATLRSRLL PQVSLITPNL PEAAALLDAP 150
    HARTEQEMLE QGRSLLAMGC GAVLMKGGHL DDEQSPDWLF TREGEQRFTA 200
    PRIMTKNTHG TGCTLSAALA ALRPRHTNWA DTVQEAKSWL SSALAQADTL 250
    EVGHGIGPVH HFHAWW 266
    Length:266
    Mass (Da):28,634
    Last modified:February 1, 1997 - v1
    Checksum:iE555CEFE445B8F0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84200 Genomic DNA. Translation: BAA76742.1.
    U00096 Genomic DNA. Translation: AAC75164.1.
    AP009048 Genomic DNA. Translation: BAA15971.1.
    PIRiF64977.
    RefSeqiNP_416606.1. NC_000913.3.
    YP_490343.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75164; AAC75164; b2103.
    BAA15971; BAA15971; BAA15971.
    GeneIDi12932542.
    946459.
    KEGGiecj:Y75_p2065.
    eco:b2103.
    PATRICi32119539. VBIEscCol129921_2180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84200 Genomic DNA. Translation: BAA76742.1 .
    U00096 Genomic DNA. Translation: AAC75164.1 .
    AP009048 Genomic DNA. Translation: BAA15971.1 .
    PIRi F64977.
    RefSeqi NP_416606.1. NC_000913.3.
    YP_490343.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76422.
    SMRi P76422. Positions 1-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6867N.
    IntActi P76422. 7 interactions.
    STRINGi 511145.b2103.

    Proteomic databases

    PaxDbi P76422.
    PRIDEi P76422.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75164 ; AAC75164 ; b2103 .
    BAA15971 ; BAA15971 ; BAA15971 .
    GeneIDi 12932542.
    946459.
    KEGGi ecj:Y75_p2065.
    eco:b2103.
    PATRICi 32119539. VBIEscCol129921_2180.

    Organism-specific databases

    EchoBASEi EB3821.
    EcoGenei EG14068. thiD.

    Phylogenomic databases

    eggNOGi COG0351.
    HOGENOMi HOG000225275.
    KOi K00941.
    OMAi TNHWAYS.
    OrthoDBi EOG6XWV53.
    PhylomeDBi P76422.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00137 .
    UPA00060 ; UER00138 .
    BioCyci EcoCyc:HMP-P-KIN-MONOMER.
    ECOL316407:JW2090-MONOMER.
    MetaCyc:HMP-P-KIN-MONOMER.

    Miscellaneous databases

    PROi P76422.

    Gene expression databases

    Genevestigatori P76422.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    InterProi IPR004399. HMP/HMP-P_kinase.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF08543. Phos_pyr_kin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00097. HMP-P_kinase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."
      Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.
      Microbiology 145:495-501(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CATALYTIC ACTIVITY, SUBUNIT.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiTHID_ECOLI
    AccessioniPrimary (citable) accession number: P76422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3