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Protein

Lipid kinase YegS

Gene

yegS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.

Cofactori

Mg2+1 Publication, Ca2+1 PublicationNote: Binds 1 Mg2+ ion per subunit. Ca2+ may be able to substitute.1 Publication

Kineticsi

  1. KM=0.7 mM for phosphatidylglycerol
  1. Vmax=29.7 nmol/min/mg enzyme

pH dependencei

Optimum pH is 7.5. Active from pH 5 to 9.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPBy similarity1
Binding sitei95ATPBy similarity1
Metal bindingi215Magnesium; via carbonyl oxygen1 Publication1
Metal bindingi218Magnesium1 Publication1
Metal bindingi220Magnesium; via carbonyl oxygen1 Publication1
Active sitei271Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 72ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • lipid kinase activity Source: EcoCyc
  • magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7123-MONOMER.
ECOL316407:JW2070-MONOMER.
MetaCyc:G7123-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid kinase YegS (EC:2.7.1.-)
Gene namesi
Name:yegS
Ordered Locus Names:b2086, JW2070
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14367. yegS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691291 – 299Lipid kinase YegSAdd BLAST299

Proteomic databases

PaxDbiP76407.
PRIDEiP76407.

Interactioni

Subunit structurei

Monomer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gltAP0ABH72EBI-1127478,EBI-547808

Protein-protein interaction databases

BioGridi4261758. 16 interactors.
DIPiDIP-11889N.
IntActiP76407. 8 interactors.
STRINGi511145.b2086.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Beta strandi13 – 15Combined sources3
Helixi19 – 29Combined sources11
Turni30 – 32Combined sources3
Beta strandi35 – 39Combined sources5
Helixi45 – 56Combined sources12
Beta strandi59 – 66Combined sources8
Helixi67 – 79Combined sources13
Beta strandi87 – 92Combined sources6
Beta strandi94 – 96Combined sources3
Helixi98 – 102Combined sources5
Helixi109 – 118Combined sources10
Beta strandi119 – 129Combined sources11
Turni130 – 132Combined sources3
Beta strandi134 – 146Combined sources13
Helixi160 – 168Combined sources9
Beta strandi172 – 176Combined sources5
Beta strandi178 – 184Combined sources7
Beta strandi187 – 202Combined sources16
Turni205 – 207Combined sources3
Beta strandi208 – 211Combined sources4
Beta strandi221 – 226Combined sources6
Helixi233 – 240Combined sources8
Beta strandi248 – 268Combined sources21
Beta strandi271 – 291Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BONX-ray1.90A/B1-299[»]
2JGRX-ray2.65A1-299[»]
ProteinModelPortaliP76407.
SMRiP76407.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76407.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 133DAGKcAdd BLAST132

Sequence similaritiesi

Contains 1 DAGKc domain.Curated

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
HOGENOMiHOG000261396.
InParanoidiP76407.
OMAiHWQGDLL.
PhylomeDBiP76407.

Family and domain databases

HAMAPiMF_01377. YegS. 1 hit.
InterProiIPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFPASLLI LNGKSTDNLP LREAIMLLRE EGMTIHVRVT WEKGDAARYV
60 70 80 90 100
EEARKFGVAT VIAGGGDGTI NEVSTALIQC EGDDIPALGI LPLGTANDFA
110 120 130 140 150
TSVGIPEALD KALKLAIAGD AIAIDMAQVN KQTCFINMAT GGFGTRITTE
160 170 180 190 200
TPEKLKAALG SVSYIIHGLM RMDTLQPDRC EIRGENFHWQ GDALVIGIGN
210 220 230 240 250
GRQAGGGQQL CPNALINDGL LQLRIFTGDE ILPALVSTLK SDEDNPNIIE
260 270 280 290
GASSWFDIQA PHDITFNLDG EPLSGQNFHI EILPAALRCR LPPDCPLLR
Length:299
Mass (Da):32,039
Last modified:February 1, 1997 - v1
Checksum:i4C90DDD8456C2829
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75147.1.
AP009048 Genomic DNA. Translation: BAA15939.1.
PIRiE64975.
RefSeqiNP_416590.1. NC_000913.3.
WP_000807348.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75147; AAC75147; b2086.
BAA15939; BAA15939; BAA15939.
GeneIDi946626.
KEGGiecj:JW2070.
eco:b2086.
PATRICi32119505. VBIEscCol129921_2163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75147.1.
AP009048 Genomic DNA. Translation: BAA15939.1.
PIRiE64975.
RefSeqiNP_416590.1. NC_000913.3.
WP_000807348.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BONX-ray1.90A/B1-299[»]
2JGRX-ray2.65A1-299[»]
ProteinModelPortaliP76407.
SMRiP76407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261758. 16 interactors.
DIPiDIP-11889N.
IntActiP76407. 8 interactors.
STRINGi511145.b2086.

Proteomic databases

PaxDbiP76407.
PRIDEiP76407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75147; AAC75147; b2086.
BAA15939; BAA15939; BAA15939.
GeneIDi946626.
KEGGiecj:JW2070.
eco:b2086.
PATRICi32119505. VBIEscCol129921_2163.

Organism-specific databases

EchoBASEiEB4111.
EcoGeneiEG14367. yegS.

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
HOGENOMiHOG000261396.
InParanoidiP76407.
OMAiHWQGDLL.
PhylomeDBiP76407.

Enzyme and pathway databases

BioCyciEcoCyc:G7123-MONOMER.
ECOL316407:JW2070-MONOMER.
MetaCyc:G7123-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76407.
PROiP76407.

Family and domain databases

HAMAPiMF_01377. YegS. 1 hit.
InterProiIPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYEGS_ECOLI
AccessioniPrimary (citable) accession number: P76407
Secondary accession number(s): O08008, O08011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.