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Protein

Lipid kinase YegS

Gene

yegS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.

Cofactori

Mg2+1 Publication, Ca2+1 PublicationNote: Binds 1 Mg2+ ion per subunit. Ca2+ may be able to substitute.1 Publication

Kineticsi

  1. KM=0.7 mM for phosphatidylglycerol
  1. Vmax=29.7 nmol/min/mg enzyme

pH dependencei

Optimum pH is 7.5. Active from pH 5 to 9.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATPBy similarity
Binding sitei95 – 951ATPBy similarity
Metal bindingi215 – 2151Magnesium; via carbonyl oxygen1 Publication
Metal bindingi218 – 2181Magnesium1 Publication
Metal bindingi220 – 2201Magnesium; via carbonyl oxygen1 Publication
Active sitei271 – 2711Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 727ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • lipid kinase activity Source: EcoCyc
  • magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  • lipid phosphorylation Source: GOC
  • phospholipid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7123-MONOMER.
ECOL316407:JW2070-MONOMER.
MetaCyc:G7123-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid kinase YegS (EC:2.7.1.-)
Gene namesi
Name:yegS
Ordered Locus Names:b2086, JW2070
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14367. yegS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Lipid kinase YegSPRO_0000169129Add
BLAST

Proteomic databases

PaxDbiP76407.
PRIDEiP76407.

Interactioni

Subunit structurei

Monomer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gltAP0ABH72EBI-1127478,EBI-547808

Protein-protein interaction databases

BioGridi4261758. 16 interactions.
DIPiDIP-11889N.
IntActiP76407. 8 interactions.
STRINGi511145.b2086.

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Beta strandi13 – 153Combined sources
Helixi19 – 2911Combined sources
Turni30 – 323Combined sources
Beta strandi35 – 395Combined sources
Helixi45 – 5612Combined sources
Beta strandi59 – 668Combined sources
Helixi67 – 7913Combined sources
Beta strandi87 – 926Combined sources
Beta strandi94 – 963Combined sources
Helixi98 – 1025Combined sources
Helixi109 – 11810Combined sources
Beta strandi119 – 12911Combined sources
Turni130 – 1323Combined sources
Beta strandi134 – 14613Combined sources
Helixi160 – 1689Combined sources
Beta strandi172 – 1765Combined sources
Beta strandi178 – 1847Combined sources
Beta strandi187 – 20216Combined sources
Turni205 – 2073Combined sources
Beta strandi208 – 2114Combined sources
Beta strandi221 – 2266Combined sources
Helixi233 – 2408Combined sources
Beta strandi248 – 26821Combined sources
Beta strandi271 – 29121Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BONX-ray1.90A/B1-299[»]
2JGRX-ray2.65A1-299[»]
ProteinModelPortaliP76407.
SMRiP76407. Positions 5-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76407.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 133132DAGKcAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.Curated

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
HOGENOMiHOG000261396.
InParanoidiP76407.
OMAiHWQGDLL.
OrthoDBiEOG613098.
PhylomeDBiP76407.

Family and domain databases

HAMAPiMF_01377. YegS.
InterProiIPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFPASLLI LNGKSTDNLP LREAIMLLRE EGMTIHVRVT WEKGDAARYV
60 70 80 90 100
EEARKFGVAT VIAGGGDGTI NEVSTALIQC EGDDIPALGI LPLGTANDFA
110 120 130 140 150
TSVGIPEALD KALKLAIAGD AIAIDMAQVN KQTCFINMAT GGFGTRITTE
160 170 180 190 200
TPEKLKAALG SVSYIIHGLM RMDTLQPDRC EIRGENFHWQ GDALVIGIGN
210 220 230 240 250
GRQAGGGQQL CPNALINDGL LQLRIFTGDE ILPALVSTLK SDEDNPNIIE
260 270 280 290
GASSWFDIQA PHDITFNLDG EPLSGQNFHI EILPAALRCR LPPDCPLLR
Length:299
Mass (Da):32,039
Last modified:February 1, 1997 - v1
Checksum:i4C90DDD8456C2829
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75147.1.
AP009048 Genomic DNA. Translation: BAA15939.1.
PIRiE64975.
RefSeqiNP_416590.1. NC_000913.3.
WP_000807348.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75147; AAC75147; b2086.
BAA15939; BAA15939; BAA15939.
GeneIDi946626.
KEGGiecj:JW2070.
eco:b2086.
PATRICi32119505. VBIEscCol129921_2163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75147.1.
AP009048 Genomic DNA. Translation: BAA15939.1.
PIRiE64975.
RefSeqiNP_416590.1. NC_000913.3.
WP_000807348.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BONX-ray1.90A/B1-299[»]
2JGRX-ray2.65A1-299[»]
ProteinModelPortaliP76407.
SMRiP76407. Positions 5-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261758. 16 interactions.
DIPiDIP-11889N.
IntActiP76407. 8 interactions.
STRINGi511145.b2086.

Proteomic databases

PaxDbiP76407.
PRIDEiP76407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75147; AAC75147; b2086.
BAA15939; BAA15939; BAA15939.
GeneIDi946626.
KEGGiecj:JW2070.
eco:b2086.
PATRICi32119505. VBIEscCol129921_2163.

Organism-specific databases

EchoBASEiEB4111.
EcoGeneiEG14367. yegS.

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
HOGENOMiHOG000261396.
InParanoidiP76407.
OMAiHWQGDLL.
OrthoDBiEOG613098.
PhylomeDBiP76407.

Enzyme and pathway databases

BioCyciEcoCyc:G7123-MONOMER.
ECOL316407:JW2070-MONOMER.
MetaCyc:G7123-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76407.
PROiP76407.

Family and domain databases

HAMAPiMF_01377. YegS.
InterProiIPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Expression, purification, crystallization and preliminary diffraction studies of the mammalian DAG kinase homologue yegS from Escherichia coli."
    Bakali H.M.A., Nordlund P., Hallberg B.M.
    Acta Crystallogr. F 62:295-297(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, CRYSTALLIZATION.
    Strain: K12.
  5. "Crystal structure of yegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site."
    Bakali H.M.A., Herman M.D., Johnson K.A., Kelly A.A., Wieslander A., Hallberg B.M., Nordlund P.
    J. Biol. Chem. 282:19644-19652(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF APOENZYME AND IN COMPLEX WITH ADP AND MAGNESIUM, CHARACTERIZATION, COFACTOR, SUBCELLULAR LOCATION.
    Strain: K12.

Entry informationi

Entry nameiYEGS_ECOLI
AccessioniPrimary (citable) accession number: P76407
Secondary accession number(s): O08008, O08011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.