Multidrug resistance protein MdtC - Escherichia coli (strain K12)

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P76399 (MDTC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Multidrug resistance protein MdtC

Alternative name(s):
Multidrug transporter MdtC

Gene names

Name:	mdtC
Synonyms:	yegO
Ordered Locus Names:	b2076, JW2061

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The MdtABC tripartite complex confers resistance against novobiocin and deoxycholate. Ref.1 Ref.5
Subunit structure	Part of a tripartite efflux system composed of MdtA, MdtB and MdtC. MdtC forms a heteromultimer with MdtB.
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_01424.
Induction	The mdtABC operon is transcriptionally activated by BaeR. Ref.1 Ref.5
Miscellaneous	MdtABC requires TolC for its function. HAMAP-Rule MF_01424
Sequence similarities	Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 20038594. Source: EcoCyc
Molecular_function	drug:hydrogen antiporter activity Inferred from direct assay PubMed 20038594. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mdtB	P76398	3	EBI-1116694,EBI-561416

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1025

1025

Multidrug resistance protein MdtC HAMAP-Rule MF_01424

PRO_0000161831

Regions

Topological domain

1 – 6

Cytoplasmic Potential

Transmembrane

7 – 29

Helical; Potential

Topological domain

30 – 335

306

Periplasmic Potential

Transmembrane

336 – 353

Helical; Potential

Topological domain

354 – 359

Cytoplasmic Potential

Transmembrane

360 – 379

Helical; Potential

Topological domain

380 – 388

Periplasmic Potential

Transmembrane

389 – 411

Helical; Potential

Topological domain

412 – 430

Cytoplasmic Potential

Transmembrane

431 – 453

Helical; Potential

Topological domain

454 – 467

Periplasmic Potential

Transmembrane

468 – 490

Helical; Potential

Topological domain

491 – 852

362

Cytoplasmic Potential

Transmembrane

853 – 875

Helical; Potential

Topological domain

876 – 894

Periplasmic Potential

Transmembrane

895 – 917

Helical; Potential

Topological domain

918 – 947

Cytoplasmic Potential

Transmembrane

948 – 970

Helical; Potential

Topological domain

971 – 984

Periplasmic Potential

Transmembrane

985 – 1007

Helical; Potential

Topological domain

1008 – 1025

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P76399 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: EF00BB4E7B301008
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FASTA

1,025

111,010

        10         20         30         40         50         60 
MKFFALFIYR PVATILLSVA ITLCGILGFR MLPVAPLPQV DFPVIIVSAS LPGASPETMA 

        70         80         90        100        110        120 
SSVATPLERS LGRIAGVSEM TSSSSLGSTR IILQFDFDRD INGAARDVQA AINAAQSLLP 

       130        140        150        160        170        180 
SGMPSRPTYR KANPSDAPIM ILTLTSDTYS QGELYDFAST QLAPTISQID GVGDVDVGGS 

       190        200        210        220        230        240 
SLPAVRVGLN PQALFNQGVS LDDVRTAVSN ANVRKPQGAL EDGTHRWQIQ TNDELKTAAE 

       250        260        270        280        290        300 
YQPLIIHYNN GGAVRLGDVA TVTDSVQDVR NAGMTNAKPA ILLMIRKLPE ANIIQTVDSI 

       310        320        330        340        350        360 
RAKLPELQET IPAAIDLQIA QDRSPTIRAS LEEVEQTLII SVALVILVVF LFLRSGRATI 

       370        380        390        400        410        420 
IPAVSVPVSL IGTFAAMYLC GFSLNNLSLM ALTIATGFVV DDAIVVLENI ARHLEAGMKP 

       430        440        450        460        470        480 
LQAALQGTRE VGFTVLSMSL SLVAVFLPLL LMGGLPGRLL REFAVTLSVA IGISLLVSLT 

       490        500        510        520        530        540 
LTPMMCGWML KASKPREQKR LRGFGRMLVA LQQGYGKSLK WVLNHTRLVG VVLLGTIALN 

       550        560        570        580        590        600 
IWLYISIPKT FFPEQDTGVL MGGIQADQSI SFQAMRGKLQ DFMKIIRDDP AVDNVTGFTG 

       610        620        630        640        650        660 
GSRVNSGMMF ITLKPRDERS ETAQQIIDRL RVKLAKEPGA NLFLMAVQDI RVGGRQSNAS 

       670        680        690        700        710        720 
YQYTLLSDDL AALREWEPKI RKKLATLPEL ADVNSDQQDN GAEMNLVYDR DTMARLGIDV 

       730        740        750        760        770        780 
QAANSLLNNA FGQRQISTIY QPMNQYKVVM EVDPRYTQDI SALEKMFVIN NEGKAIPLSY 

       790        800        810        820        830        840 
FAKWQPANAP LSVNHQGLSA ASTISFNLPT GKSLSDASAA IDRAMTQLGV PSTVRGSFAG 

       850        860        870        880        890        900 
TAQVFQETMN SQVILIIAAI ATVYIVLGIL YESYVHPLTI LSTLPSAGVG ALLALELFNA 

       910        920        930        940        950        960 
PFSLIALIGI MLLIGIVKKN AIMMVDFALE AQRHGNLTPQ EAIFQACLLR FRPIMMTTLA 

       970        980        990       1000       1010       1020 
ALFGALPLVL SGGDGSELRQ PLGITIVGGL VMSQLLTLYT TPVVYLFFDR LRLRFSRKPK 


QTVTE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC." Nagakubo S., Nishino K., Hirata T., Yamaguchi A. J. Bacteriol. 184:4161-4167(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate." Baranova N., Nikaido H. J. Bacteriol. 184:4168-4176(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB089189 Genomic DNA. Translation: BAC06609.1. U00096 Genomic DNA. Translation: AAC75137.1. AP009048 Genomic DNA. Translation: BAA15932.1.
PIR	C64974.
RefSeq	NP_416580.1. NC_000913.2. YP_490318.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76399.
SMR	P76399. Positions 6-998.
ModBase	Search...
Protein-protein interaction databases
IntAct	P76399. 4 interactions.
STRING	511145.b2076.
Protein family/group databases
TCDB	2.A.6.2.14. resistance-nodulation-cell division (RND) superfamily.
Proteomic databases
PRIDE	P76399.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75137; AAC75137; b2076. BAA15932; BAA15932; BAA15932.
GeneID	12931428. 946608.
KEGG	ecj:Y75_p2039. eco:b2076.
PATRIC	32119485. VBIEscCol129921_2153.
Organism-specific databases
EchoBASE	EB3811.
EcoGene	EG14058. mdtC.
Phylogenomic databases
eggNOG	COG0841.
HOGENOM	HOG000158127.
KO	K07789.
OMA	GQLYDFA.
ProtClustDB	PRK10614.
Enzyme and pathway databases
BioCyc	EcoCyc:B2076-MONOMER. ECOL316407:JW2061-MONOMER.
Gene expression databases
Genevestigator	P76399.
Family and domain databases
Gene3D	3.30.2090.10. 2 hits.
HAMAP	MF_01424. MdtC.
InterPro	IPR027463. AcrB_DN_DC_subdom. IPR001036. Acrflvin-R. IPR023931. Multidrug-R_MdtC. [Graphical view]
Pfam	PF00873. ACR_tran. 1 hit. [Graphical view]
PRINTS	PR00702. ACRIFLAVINRP.
SUPFAM	SSF82714. SSF82714. 2 hits.
ProtoNet	Search...

Entry information

Entry name

MDTC_ECOLI

Accession

Primary (citable) accession number: P76399
Secondary accession number(s): O08006

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents