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Protein

Multidrug resistance protein MdtC

Gene

mdtC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The MdtABC tripartite complex confers resistance against novobiocin and deoxycholate. MdtABC requires TolC for its function.UniRule annotation2 Publications

GO - Molecular functioni

  • drug:proton antiporter activity Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:B2076-MONOMER.
ECOL316407:JW2061-MONOMER.

Protein family/group databases

TCDBi2.A.6.2.12. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtCUniRule annotation
Alternative name(s):
Multidrug transporter MdtCUniRule annotation
Gene namesi
Name:mdtCUniRule annotation
Synonyms:yegO
Ordered Locus Names:b2076, JW2061
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14058. mdtC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2923HelicalUniRule annotationAdd
BLAST
Topological domaini30 – 335306PeriplasmicSequence analysisAdd
BLAST
Transmembranei336 – 35318HelicalUniRule annotationAdd
BLAST
Topological domaini354 – 3596CytoplasmicSequence analysis
Transmembranei360 – 37920HelicalUniRule annotationAdd
BLAST
Topological domaini380 – 3889PeriplasmicSequence analysis
Transmembranei389 – 41123HelicalUniRule annotationAdd
BLAST
Topological domaini412 – 43019CytoplasmicSequence analysisAdd
BLAST
Transmembranei431 – 45323HelicalUniRule annotationAdd
BLAST
Topological domaini454 – 46714PeriplasmicSequence analysisAdd
BLAST
Transmembranei468 – 49023HelicalUniRule annotationAdd
BLAST
Topological domaini491 – 852362CytoplasmicSequence analysisAdd
BLAST
Transmembranei853 – 87523HelicalUniRule annotationAdd
BLAST
Topological domaini876 – 89419PeriplasmicSequence analysisAdd
BLAST
Transmembranei895 – 91723HelicalUniRule annotationAdd
BLAST
Topological domaini918 – 94730CytoplasmicSequence analysisAdd
BLAST
Transmembranei948 – 97023HelicalUniRule annotationAdd
BLAST
Topological domaini971 – 98414PeriplasmicSequence analysisAdd
BLAST
Transmembranei985 – 100723HelicalUniRule annotationAdd
BLAST
Topological domaini1008 – 102518CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Multidrug resistance protein MdtCPRO_0000161831Add
BLAST

Proteomic databases

PaxDbiP76399.
PRIDEiP76399.

Expressioni

Inductioni

The mdtABC operon is transcriptionally activated by BaeR.UniRule annotation2 Publications

Interactioni

Subunit structurei

Part of a tripartite efflux system composed of MdtA, MdtB and MdtC. MdtC forms a heteromultimer with MdtB.

Binary interactionsi

WithEntry#Exp.IntActNotes
mdtBP763983EBI-1116694,EBI-561416

Protein-protein interaction databases

BioGridi4260422. 155 interactions.
IntActiP76399. 4 interactions.
STRINGi511145.b2076.

Structurei

3D structure databases

ProteinModelPortaliP76399.
SMRiP76399. Positions 6-998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MdtC subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158127.
InParanoidiP76399.
KOiK07789.
OMAiMFVINSD.
OrthoDBiEOG6SFP62.
PhylomeDBiP76399.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
HAMAPiMF_01424. MdtC.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR023931. Multidrug-R_MdtC.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.

Sequencei

Sequence statusi: Complete.

P76399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFFALFIYR PVATILLSVA ITLCGILGFR MLPVAPLPQV DFPVIIVSAS
60 70 80 90 100
LPGASPETMA SSVATPLERS LGRIAGVSEM TSSSSLGSTR IILQFDFDRD
110 120 130 140 150
INGAARDVQA AINAAQSLLP SGMPSRPTYR KANPSDAPIM ILTLTSDTYS
160 170 180 190 200
QGELYDFAST QLAPTISQID GVGDVDVGGS SLPAVRVGLN PQALFNQGVS
210 220 230 240 250
LDDVRTAVSN ANVRKPQGAL EDGTHRWQIQ TNDELKTAAE YQPLIIHYNN
260 270 280 290 300
GGAVRLGDVA TVTDSVQDVR NAGMTNAKPA ILLMIRKLPE ANIIQTVDSI
310 320 330 340 350
RAKLPELQET IPAAIDLQIA QDRSPTIRAS LEEVEQTLII SVALVILVVF
360 370 380 390 400
LFLRSGRATI IPAVSVPVSL IGTFAAMYLC GFSLNNLSLM ALTIATGFVV
410 420 430 440 450
DDAIVVLENI ARHLEAGMKP LQAALQGTRE VGFTVLSMSL SLVAVFLPLL
460 470 480 490 500
LMGGLPGRLL REFAVTLSVA IGISLLVSLT LTPMMCGWML KASKPREQKR
510 520 530 540 550
LRGFGRMLVA LQQGYGKSLK WVLNHTRLVG VVLLGTIALN IWLYISIPKT
560 570 580 590 600
FFPEQDTGVL MGGIQADQSI SFQAMRGKLQ DFMKIIRDDP AVDNVTGFTG
610 620 630 640 650
GSRVNSGMMF ITLKPRDERS ETAQQIIDRL RVKLAKEPGA NLFLMAVQDI
660 670 680 690 700
RVGGRQSNAS YQYTLLSDDL AALREWEPKI RKKLATLPEL ADVNSDQQDN
710 720 730 740 750
GAEMNLVYDR DTMARLGIDV QAANSLLNNA FGQRQISTIY QPMNQYKVVM
760 770 780 790 800
EVDPRYTQDI SALEKMFVIN NEGKAIPLSY FAKWQPANAP LSVNHQGLSA
810 820 830 840 850
ASTISFNLPT GKSLSDASAA IDRAMTQLGV PSTVRGSFAG TAQVFQETMN
860 870 880 890 900
SQVILIIAAI ATVYIVLGIL YESYVHPLTI LSTLPSAGVG ALLALELFNA
910 920 930 940 950
PFSLIALIGI MLLIGIVKKN AIMMVDFALE AQRHGNLTPQ EAIFQACLLR
960 970 980 990 1000
FRPIMMTTLA ALFGALPLVL SGGDGSELRQ PLGITIVGGL VMSQLLTLYT
1010 1020
TPVVYLFFDR LRLRFSRKPK QTVTE
Length:1,025
Mass (Da):111,010
Last modified:February 1, 1997 - v1
Checksum:iEF00BB4E7B301008
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089189 Genomic DNA. Translation: BAC06609.1.
U00096 Genomic DNA. Translation: AAC75137.1.
AP009048 Genomic DNA. Translation: BAA15932.1.
PIRiC64974.
RefSeqiNP_416580.1. NC_000913.3.
WP_000667481.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75137; AAC75137; b2076.
BAA15932; BAA15932; BAA15932.
GeneIDi946608.
KEGGiecj:JW2061.
eco:b2076.
PATRICi32119485. VBIEscCol129921_2153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089189 Genomic DNA. Translation: BAC06609.1.
U00096 Genomic DNA. Translation: AAC75137.1.
AP009048 Genomic DNA. Translation: BAA15932.1.
PIRiC64974.
RefSeqiNP_416580.1. NC_000913.3.
WP_000667481.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76399.
SMRiP76399. Positions 6-998.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260422. 155 interactions.
IntActiP76399. 4 interactions.
STRINGi511145.b2076.

Protein family/group databases

TCDBi2.A.6.2.12. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiP76399.
PRIDEiP76399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75137; AAC75137; b2076.
BAA15932; BAA15932; BAA15932.
GeneIDi946608.
KEGGiecj:JW2061.
eco:b2076.
PATRICi32119485. VBIEscCol129921_2153.

Organism-specific databases

EchoBASEiEB3811.
EcoGeneiEG14058. mdtC.

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158127.
InParanoidiP76399.
KOiK07789.
OMAiMFVINSD.
OrthoDBiEOG6SFP62.
PhylomeDBiP76399.

Enzyme and pathway databases

BioCyciEcoCyc:B2076-MONOMER.
ECOL316407:JW2061-MONOMER.

Miscellaneous databases

PROiP76399.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
HAMAPiMF_01424. MdtC.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR023931. Multidrug-R_MdtC.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC."
    Nagakubo S., Nishino K., Hirata T., Yamaguchi A.
    J. Bacteriol. 184:4161-4167(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate."
    Baranova N., Nikaido H.
    J. Bacteriol. 184:4168-4176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMDTC_ECOLI
AccessioniPrimary (citable) accession number: P76399
Secondary accession number(s): O08006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.