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Protein

Multidrug resistance protein MdtB

Gene

mdtB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The MdtABC tripartite complex confers resistance against novobiocin and deoxycholate. MdtABC requires TolC for its function.UniRule annotation2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:B2075-MONOMER.
ECOL316407:JW2060-MONOMER.

Protein family/group databases

TCDBi2.A.6.2.12. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtBUniRule annotation
Alternative name(s):
Multidrug transporter MdtBUniRule annotation
Gene namesi
Name:mdtBUniRule annotation
Synonyms:yegN
Ordered Locus Names:b2075, JW2060
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14057. mdtB.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3723HelicalUniRule annotationAdd
BLAST
Transmembranei345 – 36218HelicalUniRule annotationAdd
BLAST
Transmembranei367 – 38923HelicalUniRule annotationAdd
BLAST
Transmembranei396 – 41823HelicalUniRule annotationAdd
BLAST
Transmembranei438 – 46023HelicalUniRule annotationAdd
BLAST
Transmembranei472 – 49423HelicalUniRule annotationAdd
BLAST
Transmembranei535 – 55723HelicalUniRule annotationAdd
BLAST
Transmembranei867 – 88923HelicalUniRule annotationAdd
BLAST
Transmembranei909 – 93123HelicalUniRule annotationAdd
BLAST
Transmembranei968 – 99023HelicalUniRule annotationAdd
BLAST
Transmembranei1000 – 102223HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10401040Multidrug resistance protein MdtBPRO_0000161821Add
BLAST

Proteomic databases

PaxDbiP76398.
PRIDEiP76398.

Expressioni

Inductioni

The mdtABC operon is transcriptionally activated by BaeR.UniRule annotation2 Publications

Interactioni

Subunit structurei

Part of a tripartite efflux system composed of MdtA, MdtB and MdtC. MdtB forms a heteromultimer with MdtC.

Binary interactionsi

WithEntry#Exp.IntActNotes
mdtCP763993EBI-561416,EBI-1116694

Protein-protein interaction databases

BioGridi4262010. 440 interactions.
DIPiDIP-11884N.
IntActiP76398. 3 interactions.
MINTiMINT-1301811.
STRINGi511145.b2075.

Structurei

3D structure databases

ProteinModelPortaliP76398.
SMRiP76398. Positions 19-1012.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MdtB subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158127.
InParanoidiP76398.
KOiK07788.
OMAiVPMMCAK.
OrthoDBiEOG6SFP62.
PhylomeDBiP76398.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
HAMAPiMF_01423. MdtB.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR022831. Multidrug-R_MdtB.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.

Sequencei

Sequence statusi: Complete.

P76398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVLPPSSTG GPSRLFIMRP VATTLLMVAI LLAGIIGYRA LPVSALPEVD
60 70 80 90 100
YPTIQVVTLY PGASPDVMTS AVTAPLERQF GQMSGLKQMS SQSSGGASVI
110 120 130 140 150
TLQFQLTLPL DVAEQEVQAA INAATNLLPS DLPNPPVYSK VNPADPPIMT
160 170 180 190 200
LAVTSTAMPM TQVEDMVETR VAQKISQISG VGLVTLSGGQ RPAVRVKLNA
210 220 230 240 250
QAIAALGLTS ETVRTAITGA NVNSAKGSLD GPSRAVTLSA NDQMQSAEEY
260 270 280 290 300
RQLIIAYQNG APIRLGDVAT VEQGAENSWL GAWANKEQAI VMNVQRQPGA
310 320 330 340 350
NIISTADSIR QMLPQLTESL PKSVKVTVLS DRTTNIRASV DDTQFELMMA
360 370 380 390 400
IALVVMIIYL FLRNIPATII PGVAVPLSLI GTFAVMVFLD FSINNLTLMA
410 420 430 440 450
LTIATGFVVD DAIVVIENIS RYIEKGEKPL AAALKGAGEI GFTIISLTFS
460 470 480 490 500
LIAVLIPLLF MGDIVGRLFR EFAITLAVAI LISAVVSLTL TPMMCARMLS
510 520 530 540 550
QESLRKQNRF SRASEKMFDR IIAAYGRGLA KVLNHPWLTL SVALSTLLLS
560 570 580 590 600
VLLWVFIPKG FFPVQDNGII QGTLQAPQSS SFANMAQRQR QVADVILQDP
610 620 630 640 650
AVQSLTSFVG VDGTNPSLNS ARLQINLKPL DERDDRVQKV IARLQTAVDK
660 670 680 690 700
VPGVDLFLQP TQDLTIDTQV SRTQYQFTLQ ATSLDALSTW VPQLMEKLQQ
710 720 730 740 750
LPQLSDVSSD WQDKGLVAYV NVDRDSASRL GISMADVDNA LYNAFGQRLI
760 770 780 790 800
STIYTQANQY RVVLEHNTEN TPGLAALDTI RLTSSDGGVV PLSSIAKIEQ
810 820 830 840 850
RFAPLSINHL DQFPVTTISF NVPDNYSLGD AVQAIMDTEK TLNLPVDITT
860 870 880 890 900
QFQGSTLAFQ SALGSTVWLI VAAVVAMYIV LGILYESFIH PITILSTLPT
910 920 930 940 950
AGVGALLALL IAGSELDVIA IIGIILLIGI VKKNAIMMID FALAAEREQG
960 970 980 990 1000
MSPREAIYQA CLLRFRPILM TTLAALLGAL PLMLSTGVGA ELRRPLGIGM
1010 1020 1030 1040
VGGLIVSQVL TLFTTPVIYL LFDRLALWTK SRFARHEEEA
Length:1,040
Mass (Da):112,078
Last modified:February 1, 1997 - v1
Checksum:i195E3D989C7AC6F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089188 Genomic DNA. Translation: BAC06608.1.
U00096 Genomic DNA. Translation: AAC75136.1.
AP009048 Genomic DNA. Translation: BAA15929.1.
PIRiB64974.
RefSeqiNP_416579.1. NC_000913.3.
WP_001197875.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75136; AAC75136; b2075.
BAA15929; BAA15929; BAA15929.
GeneIDi946606.
KEGGiecj:JW2060.
eco:b2075.
PATRICi32119483. VBIEscCol129921_2152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089188 Genomic DNA. Translation: BAC06608.1.
U00096 Genomic DNA. Translation: AAC75136.1.
AP009048 Genomic DNA. Translation: BAA15929.1.
PIRiB64974.
RefSeqiNP_416579.1. NC_000913.3.
WP_001197875.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76398.
SMRiP76398. Positions 19-1012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262010. 440 interactions.
DIPiDIP-11884N.
IntActiP76398. 3 interactions.
MINTiMINT-1301811.
STRINGi511145.b2075.

Protein family/group databases

TCDBi2.A.6.2.12. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiP76398.
PRIDEiP76398.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75136; AAC75136; b2075.
BAA15929; BAA15929; BAA15929.
GeneIDi946606.
KEGGiecj:JW2060.
eco:b2075.
PATRICi32119483. VBIEscCol129921_2152.

Organism-specific databases

EchoBASEiEB3810.
EcoGeneiEG14057. mdtB.

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158127.
InParanoidiP76398.
KOiK07788.
OMAiVPMMCAK.
OrthoDBiEOG6SFP62.
PhylomeDBiP76398.

Enzyme and pathway databases

BioCyciEcoCyc:B2075-MONOMER.
ECOL316407:JW2060-MONOMER.

Miscellaneous databases

PROiP76398.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
HAMAPiMF_01423. MdtB.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR022831. Multidrug-R_MdtB.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC."
    Nagakubo S., Nishino K., Hirata T., Yamaguchi A.
    J. Bacteriol. 184:4161-4167(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate."
    Baranova N., Nikaido H.
    J. Bacteriol. 184:4168-4176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiMDTB_ECOLI
AccessioniPrimary (citable) accession number: P76398
Secondary accession number(s): O08005
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.