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Protein

Tyrosine-protein kinase wzc

Gene

wzc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the extracellular polysaccharide colanic acid synthesis. The autophosphorylated form is inactive. Probably involved in the export of colanic acid from the cell to medium. Phosphorylates udg.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulationi

Dephosphorylated and activated by wzb.

Pathwayi: exopolysaccharide biosynthesis

This protein is involved in the pathway exopolysaccharide biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway exopolysaccharide biosynthesis and in Glycan metabolism.

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • protein tyrosine kinase activity Source: EcoCyc

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc
  • extracellular polysaccharide biosynthetic process Source: InterPro
  • lipopolysaccharide biosynthetic process Source: InterPro
  • peptidyl-tyrosine autophosphorylation Source: EcoCyc
  • peptidyl-tyrosine phosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Exopolysaccharide synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7105-MONOMER.
ECOL316407:JW2045-MONOMER.
MetaCyc:G7105-MONOMER.
BRENDAi2.7.10.1. 2026.
UniPathwayiUPA00631.

Protein family/group databases

TCDBi8.A.3.3.2. the cytoplasmic membrane-periplasmic auxiliary-1 (mpa1) protein with cytoplasmic (c) domain (mpa1-c or mpa1+c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase wzc (EC:2.7.10.-)
Gene namesi
Name:wzc
Ordered Locus Names:b2060, JW2045
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13568. wzc.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Topological domaini53 – 424PeriplasmicSequence analysisAdd BLAST372
Transmembranei425 – 445HelicalSequence analysisAdd BLAST21
Topological domaini446 – 720CytoplasmicSequence analysisAdd BLAST275

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi467Y → F: No loss of autophosphorylation. 1 Publication1
Mutagenesisi491Y → F: No loss of autophosphorylation. 1 Publication1
Mutagenesisi540K → M: Loss of autophosphorylation. 1 Publication1
Mutagenesisi569Y → F: Loss of autophosphorylation. 1 Publication1
Mutagenesisi636Y → F: No loss of autophosphorylation. 1 Publication1
Mutagenesisi668Y → F: No loss of autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123531 – 720Tyrosine-protein kinase wzcAdd BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei569Phosphotyrosine; by autocatalysis1 Publication1
Modified residuei708Phosphotyrosine1 Publication1
Modified residuei710Phosphotyrosine1 Publication1
Modified residuei711Phosphotyrosine1 Publication1
Modified residuei713Phosphotyrosine1 Publication1
Modified residuei715Phosphotyrosine1 Publication1

Post-translational modificationi

Autophosphorylated. Seems to be phosphorylated through a cooperative two-step mechanism. First, Tyr-569 is phosphorylated in an intramolecular reaction that generates a significant increase of protein kinase activity. Then Tyr-708, Tyr-710, Tyr-711, Tyr-713 and Tyr-715 are phosphorylated in an intermolecular Tyr-569-dependent reaction.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP76387.
PRIDEiP76387.

PTM databases

iPTMnetiP76387.

Interactioni

Protein-protein interaction databases

BioGridi4262960. 334 interactors.
DIPiDIP-28075N.
IntActiP76387. 4 interactors.
MINTiMINT-8293229.
STRINGi511145.b2060.

Structurei

Secondary structure

1720
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi456 – 460Combined sources5
Turni461 – 463Combined sources3
Beta strandi466 – 471Combined sources6
Helixi474 – 478Combined sources5
Helixi497 – 500Combined sources4
Helixi505 – 520Combined sources16
Turni522 – 525Combined sources4
Beta strandi528 – 539Combined sources12
Helixi540 – 552Combined sources13
Turni553 – 555Combined sources3
Beta strandi558 – 562Combined sources5
Turni565 – 567Combined sources3
Helixi570 – 574Combined sources5
Helixi582 – 587Combined sources6
Turni592 – 595Combined sources4
Beta strandi604 – 607Combined sources4
Helixi616 – 620Combined sources5
Helixi623 – 635Combined sources13
Beta strandi637 – 642Combined sources6
Turni646 – 648Combined sources3
Helixi651 – 655Combined sources5
Turni656 – 658Combined sources3
Beta strandi660 – 667Combined sources8
Turni668 – 670Combined sources3
Helixi673 – 685Combined sources13
Beta strandi692 – 698Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LA6X-ray3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P447-720[»]
ProteinModelPortaliP76387.
SMRiP76387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the etk/wzc family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D75. Bacteria.
COG0489. LUCA.
COG3206. LUCA.
HOGENOMiHOG000153997.
InParanoidiP76387.
KOiK16692.
OMAiTKDHPAY.
PhylomeDBiP76387.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR032807. GNVR.
IPR003856. LipoPS_biosynth.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
PF13807. GNVR. 1 hit.
PF02706. Wzz. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

P76387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKVKQHAA PVTGSDEIDI GRLVGTVIEA RWWVIGITTV FALCAVVYTF
60 70 80 90 100
FATPIYSADA LVQIEQNSGN SLVQDIGSAL ANKPPASDAE IQLIRSRLVL
110 120 130 140 150
GKTVDDLDLD IAVSKNTFPI FGAGWDRLMG RQNETVKVTT FNRPKEMADQ
160 170 180 190 200
VFTLNVLDNK NYTLSSDGGF SARGQAGQML KKEGVTLMVE AIHASPGSEF
210 220 230 240 250
TVTKYSTLGM INQLQNSLTV TENGKDAGVL SLTYTGEDRE QIRDILNSIA
260 270 280 290 300
RNYQEQNIER KSAEASKSLA FLAQQLPEVR SRLDVAENKL NAFRQDKDSV
310 320 330 340 350
DLPLEAKAVL DSMVNIDAQL NELTFKEAEI SKLYTKVHPA YRTLLEKRQA
360 370 380 390 400
LEDEKAKLNG RVTAMPKTQQ EIVRLTRDVE SGQQVYMQLL NKEQELKITE
410 420 430 440 450
ASTVGDVRIV DPAITQPGVL KPKKGLIILG AIILGLMLSI VGVLLRSLFN
460 470 480 490 500
RGIESPQVLE EHGISVYASI PLSEWQKARD SVKTIKGIKR YKQSQLLAVG
510 520 530 540 550
NPTDLAIEAI RSLRTSLHFA MMQAQNNVLM MTGVSPSIGK TFVCANLAAV
560 570 580 590 600
ISQTNKRVLL IDCDMRKGYT HELLGTNNVN GLSEILIGQG DITTAAKPTS
610 620 630 640 650
IAKFDLIPRG QVPPNPSELL MSERFAELVN WASKNYDLVL IDTPPILAVT
660 670 680 690 700
DAAIVGRHVG TTLMVARYAV NTLKEVETSL SRFEQNGIPV KGVILNSIFR
710 720
RASAYQDYGY YEYEYKSDAK
Length:720
Mass (Da):79,343
Last modified:April 3, 2002 - v2
Checksum:i8F61017B5ECFFB45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77835.1.
U00096 Genomic DNA. Translation: AAC75121.2.
AP009048 Genomic DNA. Translation: BAA15913.1.
PIRiC64972.
RefSeqiNP_416564.4. NC_000913.3.
WP_000137196.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75121; AAC75121; b2060.
BAA15913; BAA15913; BAA15913.
GeneIDi946567.
KEGGiecj:JW2045.
eco:b2060.
PATRICi32119453. VBIEscCol129921_2137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77835.1.
U00096 Genomic DNA. Translation: AAC75121.2.
AP009048 Genomic DNA. Translation: BAA15913.1.
PIRiC64972.
RefSeqiNP_416564.4. NC_000913.3.
WP_000137196.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LA6X-ray3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P447-720[»]
ProteinModelPortaliP76387.
SMRiP76387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262960. 334 interactors.
DIPiDIP-28075N.
IntActiP76387. 4 interactors.
MINTiMINT-8293229.
STRINGi511145.b2060.

Protein family/group databases

TCDBi8.A.3.3.2. the cytoplasmic membrane-periplasmic auxiliary-1 (mpa1) protein with cytoplasmic (c) domain (mpa1-c or mpa1+c) family.

PTM databases

iPTMnetiP76387.

Proteomic databases

PaxDbiP76387.
PRIDEiP76387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75121; AAC75121; b2060.
BAA15913; BAA15913; BAA15913.
GeneIDi946567.
KEGGiecj:JW2045.
eco:b2060.
PATRICi32119453. VBIEscCol129921_2137.

Organism-specific databases

EchoBASEiEB3338.
EcoGeneiEG13568. wzc.

Phylogenomic databases

eggNOGiENOG4105D75. Bacteria.
COG0489. LUCA.
COG3206. LUCA.
HOGENOMiHOG000153997.
InParanoidiP76387.
KOiK16692.
OMAiTKDHPAY.
PhylomeDBiP76387.

Enzyme and pathway databases

UniPathwayiUPA00631.
BioCyciEcoCyc:G7105-MONOMER.
ECOL316407:JW2045-MONOMER.
MetaCyc:G7105-MONOMER.
BRENDAi2.7.10.1. 2026.

Miscellaneous databases

PROiP76387.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR032807. GNVR.
IPR003856. LipoPS_biosynth.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
PF13807. GNVR. 1 hit.
PF02706. Wzz. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiWZC_ECOLI
AccessioniPrimary (citable) accession number: P76387
Secondary accession number(s): O08003, O08004, P71236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 3, 2002
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Additional site-directed mutagenesis experiments indicated that the tyrosine residues at positions 708, 710, 711, 713 and 715 are phosphorylation sites, whereas tyrosine at position 705 is not.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.