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Protein

Cytoskeleton bundling-enhancing protein CbeA

Gene

cbeA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a type IV toxin-antitoxin (TA) module. Labile antitoxin that counteracts the effect of its cognate toxin CbtA (YeeV). It does not bind to the toxin but instead binds to MreB and FtsZ (the toxin targets), enhancing their polymerization by forming higher-order bundles; it is probably retained in the MreB and FtsZ filament bundles. The mechanism has been proposed to require intergenic DNA, in cis, between the cbeA (yeeU) and cbta (yeeV) genes (PubMed:14594833). The intergenic region was not found to be necessary in another study (PubMed:22515815). Also counteracts the morphological defects caused by overexpression of SulA and DicB on cell shape.2 Publications

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi86Magnesium; via carbonyl oxygen1 Publication1
Metal bindingi88Magnesium; via amide nitrogen and carbonyl oxygen1 Publication1
Metal bindingi102Magnesium1 Publication1

GO - Molecular functioni

  • cytoskeletal protein binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of cytoskeleton organization Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7084-MONOMER.
ECOL316407:JW1986-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton bundling-enhancing protein CbeACurated
Alternative name(s):
Antitoxin CbeA
Antitoxin YeeU1 Publication
Gene namesi
Name:cbeA
Synonyms:yeeU
Ordered Locus Names:b2004, JW1986
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13388. cbeA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691151 – 122Cytoskeleton bundling-enhancing protein CbeAAdd BLAST122

Proteomic databases

PaxDbiP76364.
PRIDEiP76364.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with polymerized and monomeric MreB and polymerized FtsZ.Curated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsZP0A9A62EBI-1126877,EBI-370963
mreBP0A9X42EBI-1126877,EBI-371008

GO - Molecular functioni

  • cytoskeletal protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261519. 12 interactors.
IntActiP76364. 6 interactors.
STRINGi511145.b2004.

Structurei

Secondary structure

1122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 37Combined sources11
Beta strandi40 – 42Combined sources3
Helixi45 – 47Combined sources3
Beta strandi48 – 52Combined sources5
Helixi56 – 79Combined sources24
Beta strandi80 – 82Combined sources3
Beta strandi90 – 94Combined sources5
Beta strandi97 – 102Combined sources6
Beta strandi107 – 116Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H28X-ray2.10A/B1-122[»]
ProteinModelPortaliP76364.
SMRiP76364.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76364.

Family & Domainsi

Sequence similaritiesi

Belongs to the CbeA/YafW/YfjZ antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105MAW. Bacteria.
ENOG4111V4B. LUCA.
HOGENOMiHOG000290944.
KOiK18838.
OMAiKQPELML.
PhylomeDBiP76364.

Family and domain databases

InterProiIPR009320. YagB_YeeU_YfjZ.
[Graphical view]
PfamiPF06154. CbeA_antitoxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTLPGTTL PDDNHDRPWW GLPCTVTPCF GARLVQEGNR LHYLADRAGI
60 70 80 90 100
RGLFSDADAY HLDQAFPLLM KQLELMLTSG ELNPRHQHTV TLYAKGLTCK
110 120
ADTLSSCDYV YLAVYPTPEM KN
Length:122
Mass (Da):13,684
Last modified:February 1, 1997 - v1
Checksum:iE6F703DEA3D67290
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75065.1.
AP009048 Genomic DNA. Translation: BAA15827.1.
PIRiC64965.
RefSeqiNP_416508.1. NC_000913.3.
WP_001285584.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75065; AAC75065; b2004.
BAA15827; BAA15827; BAA15827.
GeneIDi946510.
KEGGiecj:JW1986.
eco:b2004.
PATRICi32119341. VBIEscCol129921_2080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75065.1.
AP009048 Genomic DNA. Translation: BAA15827.1.
PIRiC64965.
RefSeqiNP_416508.1. NC_000913.3.
WP_001285584.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H28X-ray2.10A/B1-122[»]
ProteinModelPortaliP76364.
SMRiP76364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261519. 12 interactors.
IntActiP76364. 6 interactors.
STRINGi511145.b2004.

Proteomic databases

PaxDbiP76364.
PRIDEiP76364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75065; AAC75065; b2004.
BAA15827; BAA15827; BAA15827.
GeneIDi946510.
KEGGiecj:JW1986.
eco:b2004.
PATRICi32119341. VBIEscCol129921_2080.

Organism-specific databases

EchoBASEiEB3169.
EcoGeneiEG13388. cbeA.

Phylogenomic databases

eggNOGiENOG4105MAW. Bacteria.
ENOG4111V4B. LUCA.
HOGENOMiHOG000290944.
KOiK18838.
OMAiKQPELML.
PhylomeDBiP76364.

Enzyme and pathway databases

BioCyciEcoCyc:G7084-MONOMER.
ECOL316407:JW1986-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76364.
PROiP76364.

Family and domain databases

InterProiIPR009320. YagB_YeeU_YfjZ.
[Graphical view]
PfamiPF06154. CbeA_antitoxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBEA_ECOLI
AccessioniPrimary (citable) accession number: P76364
Secondary accession number(s): O07991, O07994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.