P76364 (YEEU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Antitoxin YeeU

Gene names

Name:	yeeU
Ordered Locus Names:	b2004, JW1986

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antitoxin component of a toxin-antitoxin (TA) module. Labile antitoxin that counteracts the effect of the CbtA (YeeV) toxin. It does not seem to bind to the cognate toxin but instead induces loss of the toxin by an unknown mechanism that requires 68 nucleotides of intergenic DNA downstream of the antitoxin gene, in cis, between the yeeU and cbta (yeeV) genes. Ref.4
Sequence similarities	Belongs to the YeeU/YafW/YfjZ antitoxin family.

Ontologies

Keywords
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of cell killing Inferred from genetic interaction Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 122

122

Antitoxin YeeU

PRO_0000169115

Secondary structure

1

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122

Helix Strand Turn

Details...

Beta strand

27 – 37

11

Beta strand

40 – 42

3

Helix

45 – 47

3

Beta strand

48 – 52

5

Helix

56 – 79

24

Beta strand

80 – 82

3

Beta strand

90 – 94

5

Beta strand

97 – 102

6

Beta strand

107 – 116

10

Sequences

	Sequence		Length	Mass (Da)
	P76364 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E6F703DEA3D67290	FASTA	122	13,684
	10 20 30 40 50 60 MSDTLPGTTL PDDNHDRPWW GLPCTVTPCF GARLVQEGNR LHYLADRAGI RGLFSDADAY 70 80 90 100 110 120 HLDQAFPLLM KQLELMLTSG ELNPRHQHTV TLYAKGLTCK ADTLSSCDYV YLAVYPTPEM KN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"A novel family of Escherichia coli toxin-antitoxin gene pairs." Brown J.M., Shaw K.J. J. Bacteriol. 185:6600-6608(2003) [PubMed: 14594833] [Abstract] Cited for: FUNCTION AS AN ANTITOXIN. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems." Arbing M.A., Handelman S.K., Kuzin A.P., Verdon G., Wang C., Su M., Rothenbacher F.P., Abashidze M., Liu M., Hurley J.M., Xiao R., Acton T., Inouye M., Montelione G.T., Woychik N.A., Hunt J.F. Structure 18:996-1010(2010) [PubMed: 20696400] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC75065.1.
AP009048 Genomic DNA. Translation: BAA15827.1.

PIR

C64965.

RefSeq

NP_416508.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H28	X-ray	2.10	A/B	1-122	[»]

ProteinModelPortal

P76364.

SMR

P76364. Positions 16-122.

ModBase

Search...

Protein-protein interaction databases

IntAct

P76364. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002847; EBESCP00000002847; EBESCG00000002323.
EBESCT00000016187; EBESCP00000015478; EBESCG00000015247.

GeneID

946510.

GenomeReviews

Gene locus JW1986 in contig AP009048_GR.
Gene locus b2004 in contig U00096_GR.

KEGG

ecj:JW1986.
eco:b2004.

PATRIC

32119341. VBIEscCol129921_2080.

Organism-specific databases

EchoBASE

EB3169.

EcoGene

EG13388. yeeU.

Phylogenomic databases

eggNOG

NOG27515.

GeneTree

EBGT00050000010602.

HOGENOM

HBG299005.

OMA

MVTPCFG.

PhylomeDB

P76364.

ProtClustDB

CLSK879841.

Enzyme and pathway databases

BioCyc

EcoCyc:G7084-MONOMER.

Gene expression databases

Genevestigator

P76364.

Family and domain databases

InterPro

IPR009320. YagB_YeeU_YfjZ.
[Graphical view]

Pfam

PF06154. YagB_YeeU_YfjZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YEEU_ECOLI

Accession

Primary (citable) accession number: P76364
Secondary accession number(s): O07991, O07994

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program