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Protein

Cytoskeleton bundling-enhancing protein CbeA

Gene

cbeA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a type IV toxin-antitoxin (TA) module. Labile antitoxin that counteracts the effect of its cognate toxin CbtA (YeeV). It does not bind to the toxin but instead binds to MreB and FtsZ (the toxin targets), enhancing their polymerization by forming higher-order bundles; it is probably retained in the MreB and FtsZ filament bundles. The mechanism has been proposed to require intergenic DNA, in cis, between the cbeA (yeeU) and cbta (yeeV) genes (PubMed:14594833). The intergenic region was not found to be necessary in another study (PubMed:22515815). Also counteracts the morphological defects caused by overexpression of SulA and DicB on cell shape.2 Publications

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Magnesium; via carbonyl oxygen1 Publication
Metal bindingi88 – 881Magnesium; via amide nitrogen and carbonyl oxygen1 Publication
Metal bindingi102 – 1021Magnesium1 Publication

GO - Molecular functioni

  • cytoskeletal protein binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of cytoskeleton organization Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7084-MONOMER.
ECOL316407:JW1986-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton bundling-enhancing protein CbeACurated
Alternative name(s):
Antitoxin CbeA
Antitoxin YeeU1 Publication
Gene namesi
Name:cbeA
Synonyms:yeeU
Ordered Locus Names:b2004, JW1986
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13388. cbeA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 122122Cytoskeleton bundling-enhancing protein CbeAPRO_0000169115Add
BLAST

Proteomic databases

PaxDbiP76364.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with polymerized and monomeric MreB and polymerized FtsZ.Curated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsZP0A9A62EBI-1126877,EBI-370963
mreBP0A9X42EBI-1126877,EBI-371008

GO - Molecular functioni

  • cytoskeletal protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261519. 12 interactions.
IntActiP76364. 6 interactions.
STRINGi511145.b2004.

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 3711Combined sources
Beta strandi40 – 423Combined sources
Helixi45 – 473Combined sources
Beta strandi48 – 525Combined sources
Helixi56 – 7924Combined sources
Beta strandi80 – 823Combined sources
Beta strandi90 – 945Combined sources
Beta strandi97 – 1026Combined sources
Beta strandi107 – 11610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H28X-ray2.10A/B1-122[»]
ProteinModelPortaliP76364.
SMRiP76364. Positions 16-122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76364.

Family & Domainsi

Sequence similaritiesi

Belongs to the CbeA/YafW/YfjZ antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105MAW. Bacteria.
ENOG4111V4B. LUCA.
HOGENOMiHOG000290944.
KOiK18838.
OMAiKQPELML.
OrthoDBiEOG66HVJ7.
PhylomeDBiP76364.

Family and domain databases

InterProiIPR009320. YagB_YeeU_YfjZ.
[Graphical view]
PfamiPF06154. CbeA_antitoxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTLPGTTL PDDNHDRPWW GLPCTVTPCF GARLVQEGNR LHYLADRAGI
60 70 80 90 100
RGLFSDADAY HLDQAFPLLM KQLELMLTSG ELNPRHQHTV TLYAKGLTCK
110 120
ADTLSSCDYV YLAVYPTPEM KN
Length:122
Mass (Da):13,684
Last modified:February 1, 1997 - v1
Checksum:iE6F703DEA3D67290
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75065.1.
AP009048 Genomic DNA. Translation: BAA15827.1.
PIRiC64965.
RefSeqiNP_416508.1. NC_000913.3.
WP_001285584.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75065; AAC75065; b2004.
BAA15827; BAA15827; BAA15827.
GeneIDi946510.
KEGGiecj:JW1986.
eco:b2004.
PATRICi32119341. VBIEscCol129921_2080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75065.1.
AP009048 Genomic DNA. Translation: BAA15827.1.
PIRiC64965.
RefSeqiNP_416508.1. NC_000913.3.
WP_001285584.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H28X-ray2.10A/B1-122[»]
ProteinModelPortaliP76364.
SMRiP76364. Positions 16-122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261519. 12 interactions.
IntActiP76364. 6 interactions.
STRINGi511145.b2004.

Proteomic databases

PaxDbiP76364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75065; AAC75065; b2004.
BAA15827; BAA15827; BAA15827.
GeneIDi946510.
KEGGiecj:JW1986.
eco:b2004.
PATRICi32119341. VBIEscCol129921_2080.

Organism-specific databases

EchoBASEiEB3169.
EcoGeneiEG13388. cbeA.

Phylogenomic databases

eggNOGiENOG4105MAW. Bacteria.
ENOG4111V4B. LUCA.
HOGENOMiHOG000290944.
KOiK18838.
OMAiKQPELML.
OrthoDBiEOG66HVJ7.
PhylomeDBiP76364.

Enzyme and pathway databases

BioCyciEcoCyc:G7084-MONOMER.
ECOL316407:JW1986-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76364.
PROiP76364.

Family and domain databases

InterProiIPR009320. YagB_YeeU_YfjZ.
[Graphical view]
PfamiPF06154. CbeA_antitoxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "A novel family of Escherichia coli toxin-antitoxin gene pairs."
    Brown J.M., Shaw K.J.
    J. Bacteriol. 185:6600-6608(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ANTITOXIN.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli."
    Masuda H., Tan Q., Awano N., Wu K.P., Inouye M.
    Mol. Microbiol. 84:979-989(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ANTITOXIN, FUNCTION IN CYTOSKELETON BUNDLING, INTERACTION WITH MREB AND FTSZ.
    Strain: K12 / BW25113.
  6. "Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems."
    Arbing M.A., Handelman S.K., Kuzin A.P., Verdon G., Wang C., Su M., Rothenbacher F.P., Abashidze M., Liu M., Hurley J.M., Xiao R., Acton T., Inouye M., Montelione G.T., Woychik N.A., Hunt J.F.
    Structure 18:996-1010(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiCBEA_ECOLI
AccessioniPrimary (citable) accession number: P76364
Secondary accession number(s): O07991, O07994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.