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Protein

Probable FMN/FAD exporter YeeO

Gene

yeeO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A transporter able to export peptides and flavins. When overexpressed allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth (PubMed:20067529). Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide, overproduction of this protein increases its export (PubMed:20067529). When overexpressed increases secretion of FMN and FAD but not riboflavin; intracellular concentrations of FMN and riboflavin rise, possibly to compensate for increased secretion (PubMed:25482085). Increased overexpression causes slight cell elongation (PubMed:25482085).2 Publications

GO - Molecular functioni

  • antiporter activity Source: InterPro
  • dipeptide transmembrane transporter activity Source: EcoCyc
  • drug transmembrane transporter activity Source: GO_Central
  • FAD transmembrane transporter activity Source: EcoCyc
  • FMN transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • dipeptide transmembrane transport Source: EcoCyc
  • drug transmembrane transport Source: GO_Central
  • FAD transmembrane transport Source: EcoCyc
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN

Enzyme and pathway databases

BioCyciEcoCyc:YEEO-MONOMER.
ECOL316407:JW1965-MONOMER.
MetaCyc:YEEO-MONOMER.

Protein family/group databases

TCDBi2.A.66.1.23. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FMN/FAD exporter YeeO
Gene namesi
Name:yeeO
Ordered Locus Names:b1985, JW1965
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13383. yeeO.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Transmembranei139 – 159HelicalSequence analysisAdd BLAST21
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Transmembranei246 – 268HelicalSequence analysisAdd BLAST23
Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Transmembranei350 – 370HelicalSequence analysisAdd BLAST21
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Transmembranei439 – 459HelicalSequence analysisAdd BLAST21
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001642531 – 547Probable FMN/FAD exporter YeeOAdd BLAST547

Proteomic databases

PaxDbiP76352.
PRIDEiP76352.

Interactioni

Protein-protein interaction databases

BioGridi4259355. 153 interactors.
STRINGi511145.b1985.

Structurei

3D structure databases

ProteinModelPortaliP76352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105UQX. Bacteria.
COG0534. LUCA.
HOGENOMiHOG000056139.
InParanoidiP76352.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequencei

Sequence statusi: Complete.

P76352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRHILTAKN LLSNPIFKFP NCLPFLSTVC CICRQFVGEN LCSFADSPSL
60 70 80 90 100
FEMWFHFLQL RSALNISSAL RQVVHGTRWH AKRKSYKVLF WREITPLAVP
110 120 130 140 150
IFMENACVLL MGVLSTFLVS WLGKDAMAGV GLADSFNMVI MAFFAAIDLG
160 170 180 190 200
TTVVVAFSLG KRDRRRARVA TRQSLVIMTL FAVLLATLIH HFGEQIIDFV
210 220 230 240 250
AGDATTEVKA LALTYLELTV LSYPAAAITL IGSGALRGAG NTKIPLLING
260 270 280 290 300
SLNILNIIIS GILIYGLFSW PGLGFVGAGL GLTISRYIGA VAILWVLAIG
310 320 330 340 350
FNPALRISLK SYFKPLNFSI IWEVMGIGIP ASVESVLFTS GRLLTQMFVA
360 370 380 390 400
GMGTSVIAGN FIAFSIAALI NLPGSALGSA STIITGRRLG VGQIAQAEIQ
410 420 430 440 450
LRHVFWLSTL GLTAIAWLTA PFAGVMASFY TQDPQVKHVV VILIWLNALF
460 470 480 490 500
MPIWSASWVL PAGFKGARDA RYAMWVSMLS MWGCRVVVGY VLGIMLGWGV
510 520 530 540
VGVWMGMFAD WAVRAVLFYW RMVTGRWLWK YPRPEPQKCE KKPVVSE
Length:547
Mass (Da):60,061
Last modified:September 7, 2016 - v4
Checksum:i64239AEB52A0AC09
GO

Sequence cautioni

The sequence AAC75048 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75048.2. Different initiation.
AP009048 Genomic DNA. Translation: BAA15804.2.
PIRiB64963.
RefSeqiNP_416491.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75048; AAC75048; b1985.
BAA15804; BAA15804; BAA15804.
GeneIDi946506.
KEGGiecj:JW1965.
eco:b1985.
PATRICi32119301. VBIEscCol129921_2062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75048.2. Different initiation.
AP009048 Genomic DNA. Translation: BAA15804.2.
PIRiB64963.
RefSeqiNP_416491.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP76352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259355. 153 interactors.
STRINGi511145.b1985.

Protein family/group databases

TCDBi2.A.66.1.23. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Proteomic databases

PaxDbiP76352.
PRIDEiP76352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75048; AAC75048; b1985.
BAA15804; BAA15804; BAA15804.
GeneIDi946506.
KEGGiecj:JW1965.
eco:b1985.
PATRICi32119301. VBIEscCol129921_2062.

Organism-specific databases

EchoBASEiEB3164.
EcoGeneiEG13383. yeeO.

Phylogenomic databases

eggNOGiENOG4105UQX. Bacteria.
COG0534. LUCA.
HOGENOMiHOG000056139.
InParanoidiP76352.

Enzyme and pathway databases

BioCyciEcoCyc:YEEO-MONOMER.
ECOL316407:JW1965-MONOMER.
MetaCyc:YEEO-MONOMER.

Miscellaneous databases

PROiP76352.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYEEO_ECOLI
AccessioniPrimary (citable) accession number: P76352
Secondary accession number(s): P94752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 7, 2016
Last modified: November 2, 2016
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.