Shikimate transporter - Escherichia coli (strain K12)

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P76350 (SHIA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Shikimate transporter

Gene names

Name:	shiA
Synonyms:	yeeM
Ordered Locus Names:	b1981, JW1962

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	shikimate transmembrane transporter activity Inferred from mutant phenotype Ref.1. Source: EcoCyc symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 438

438

Shikimate transporter

PRO_0000050336

Regions

Topological domain

1 – 27

Cytoplasmic Potential

Transmembrane

28 – 48

Helical; Name=1; Potential

Topological domain

49 – 63

Periplasmic Potential

Transmembrane

64 – 84

Helical; Name=2; Potential

Topological domain

85 – 108

Cytoplasmic Potential

Transmembrane

109 – 129

Helical; Name=3; Potential

Topological domain

130 – 132

Periplasmic Potential

Transmembrane

133 – 153

Helical; Name=4; Potential

Topological domain

154 – 167

Cytoplasmic Potential

Transmembrane

168 – 188

Helical; Name=5; Potential

Topological domain

189 – 192

Periplasmic Potential

Transmembrane

193 – 213

Helical; Name=6; Potential

Topological domain

214 – 254

Cytoplasmic Potential

Transmembrane

255 – 275

Helical; Name=7; Potential

Topological domain

276 – 286

Periplasmic Potential

Transmembrane

287 – 307

Helical; Name=8; Potential

Topological domain

308 – 317

Cytoplasmic Potential

Transmembrane

318 – 337

Helical; Name=9; Potential

Topological domain

338 – 340

Periplasmic Potential

Transmembrane

341 – 363

Helical; Name=10; Potential

Topological domain

364 – 386

Cytoplasmic Potential

Transmembrane

387 – 407

Helical; Name=11; Potential

Topological domain

408 – 410

Periplasmic Potential

Transmembrane

411 – 431

Helical; Name=12; Potential

Topological domain

432 – 438

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P76350 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: B7FFBBD5AFDAE8CC
Show »

FASTA

438

47,817

        10         20         30         40         50         60 
MDSTLISTRP DEGTLSLSRA RRAALGSFAG AVVDWYDFLL YGITAALVFN REFFPQVSPA 

        70         80         90        100        110        120 
MGTLAAFATF GVGFLFRPLG GVIFGHFGDR LGRKRMLMLT VWMMGIATAL IGILPSFSTI 

       130        140        150        160        170        180 
GWWAPILLVT LRAIQGFAVG GEWGGAALLS VESAPKNKKA FYSSGVQVGY GVGLLLSTGL 

       190        200        210        220        230        240 
VSLISMMTTD EQFLSWGWRI PFLFSIVLVL GALWVRNGME ESAEFEQQQH YQAAAKKRIP 

       250        260        270        280        290        300 
VIEALLRHPG AFLKIIALRL CELLTMYIVT AFALNYSTQN MGLPRELFLN IGLLVGGLSC 

       310        320        330        340        350        360 
LTIPCFAWLA DRFGRRRVYI TGTLIGTLSA FPFFMALEAQ SIFWIVFFSI MLANIAHDMV 

       370        380        390        400        410        420 
VCVQQPMFTE MFGASYRYSG AGVGYQVASV VGGGFTPFIA AALITYFAGN WHSVAIYLLA 

       430 
GCLISAMTAL LMKDSQRA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and analysis of the shiA gene, which encodes the shikimate transport system of Escherichia coli K-12." Whipp M.J., Camakaris H., Pittard A.J. Gene 209:185-192(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U88529 Genomic DNA. Translation: AAC46271.1. U00096 Genomic DNA. Translation: AAC75045.1. AP009048 Genomic DNA. Translation: BAA15801.1.
PIR	G64962.
RefSeq	NP_416488.1. NC_000913.2. YP_490227.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76350.
SMR	P76350. Positions 80-111.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1981.
Protein family/group databases
TCDB	2.A.1.6.6. major facilitator superfamily (MFS).
Proteomic databases
PRIDE	P76350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75045; AAC75045; b1981. BAA15801; BAA15801; BAA15801.
GeneID	12931406. 946495.
KEGG	ecj:Y75_p1945. eco:b1981.
PATRIC	32119293. VBIEscCol129921_2059.
Organism-specific databases
EchoBASE	EB4148.
EcoGene	EG20205. shiA.
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000141611.
KO	K08172.
OMA	PMFTELF.
ProtClustDB	PRK09952.
Enzyme and pathway databases
BioCyc	EcoCyc:SHIA-MONOMER. ECOL316407:JW1962-MONOMER.
Gene expression databases
Genevestigator	P76350.
Family and domain databases
InterPro	IPR004736. Cit_H_symport. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00883. 2A0106. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. False negative. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SHIA_ECOLI

Accession

Primary (citable) accession number: P76350

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents