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Protein

Metal-binding protein ZinT

Gene

zinT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a periplasmic zinc chaperone or mediate direct transport of zinc from the periplasm to the cytoplasm under zinc-limited conditions. Binds zinc with high affinity, and can also bind cadmium, mercury or nickel. Preferentially binds Zn2+ over Cd2+. Contains one high affinity metal binding site, and can bind additional metal ions at other sites.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi167Zinc 13 Publications1
Metal bindingi176Zinc 13 Publications1
Metal bindingi176Zinc 23 Publications1
Metal bindingi178Zinc 13 Publications1
Metal bindingi212Zinc 23 Publications1
Metal bindingi216Zinc 23 Publications1

GO - Molecular functioni

  • cadmium ion binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • cellular response to zinc ion starvation Source: EcoCyc
  • response to cadmium ion Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

Cadmium, Metal-binding, Nickel, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7061-MONOMER.
ECOL316407:JW1956-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Metal-binding protein ZinT
Alternative name(s):
Cadmium-induced protein ZinT
Gene namesi
Name:zinT
Synonyms:yodA
Ordered Locus Names:b1973, JW1956
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14049. zinT.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Periplasm 1 Publication

  • Note: Cytoplasmic in nonstressed cells, and exported to the periplasm upon exposure to cadmium.

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Periplasm

Pathology & Biotechi

Disruption phenotypei

Mutant shows a growth defect and a lowered cellular Zn2+ level under Zn2+ limitation. Mutant does not bind cadmium.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001386524 – 216Metal-binding protein ZinTAdd BLAST193

Proteomic databases

PaxDbiP76344.
PRIDEiP76344.

2D gel databases

SWISS-2DPAGEP76344.

Expressioni

Inductioni

Repressed by Zur. Up-regulated in response to extreme Zn2+ deprivation. Also induced by cadmium and hydrogen peroxide.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi4260387. 36 interactors.
IntActiP76344. 15 interactors.
STRINGi511145.b1973.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 41Combined sources7
Helixi47 – 49Combined sources3
Helixi55 – 58Combined sources4
Beta strandi60 – 65Combined sources6
Helixi66 – 70Combined sources5
Turni71 – 74Combined sources4
Helixi75 – 84Combined sources10
Helixi90 – 101Combined sources12
Beta strandi104 – 111Combined sources8
Beta strandi114 – 119Combined sources6
Beta strandi122 – 126Combined sources5
Beta strandi129 – 137Combined sources9
Beta strandi143 – 150Combined sources8
Beta strandi160 – 165Combined sources6
Beta strandi175 – 184Combined sources10
Helixi186 – 190Combined sources5
Beta strandi198 – 201Combined sources4
Helixi206 – 215Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OEEX-ray2.10A24-216[»]
1OEJX-ray1.81A24-216[»]
1OEKX-ray2.40A24-216[»]
1S7DX-ray2.17A1-216[»]
1TXLX-ray1.70A2-216[»]
5AQ6X-ray1.79A24-216[»]
ProteinModelPortaliP76344.
SMRiP76344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76344.

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. ZinT family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108MEK. Bacteria.
COG3443. LUCA.
HOGENOMiHOG000219533.
InParanoidiP76344.
OMAiVQFSDHT.
PhylomeDBiP76344.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR015304. ZinT_dom.
[Graphical view]
PfamiPF09223. ZinT. 1 hit.
[Graphical view]
SUPFAMiSSF50814. SSF50814. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRLYKLAV ALGVFIVSAP AFSHGHHSHG KPLTEVEQKA ANGVFDDANV
60 70 80 90 100
QNRTLSDWDG VWQSVYPLLQ SGKLDPVFQK KADADKTKTF AEIKDYYHKG
110 120 130 140 150
YATDIEMIGI EDGIVEFHRN NETTSCKYDY DGYKILTYKS GKKGVRYLFE
160 170 180 190 200
CKDPESKAPK YIQFSDHIIA PRKSSHFHIF MGNDSQQSLL NEMENWPTYY
210
PYQLSSEEVV EEMMSH
Length:216
Mass (Da):24,762
Last modified:February 1, 1997 - v1
Checksum:iEBEE9DC73513B9C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75039.1.
AP009048 Genomic DNA. Translation: BAE76561.1.
PIRiA64962.
RefSeqiNP_416482.1. NC_000913.3.
WP_001007805.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75039; AAC75039; b1973.
BAE76561; BAE76561; BAE76561.
GeneIDi946480.
KEGGiecj:JW1956.
eco:b1973.
PATRICi32119277. VBIEscCol129921_2053.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75039.1.
AP009048 Genomic DNA. Translation: BAE76561.1.
PIRiA64962.
RefSeqiNP_416482.1. NC_000913.3.
WP_001007805.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OEEX-ray2.10A24-216[»]
1OEJX-ray1.81A24-216[»]
1OEKX-ray2.40A24-216[»]
1S7DX-ray2.17A1-216[»]
1TXLX-ray1.70A2-216[»]
5AQ6X-ray1.79A24-216[»]
ProteinModelPortaliP76344.
SMRiP76344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260387. 36 interactors.
IntActiP76344. 15 interactors.
STRINGi511145.b1973.

2D gel databases

SWISS-2DPAGEP76344.

Proteomic databases

PaxDbiP76344.
PRIDEiP76344.

Protocols and materials databases

DNASUi946480.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75039; AAC75039; b1973.
BAE76561; BAE76561; BAE76561.
GeneIDi946480.
KEGGiecj:JW1956.
eco:b1973.
PATRICi32119277. VBIEscCol129921_2053.

Organism-specific databases

EchoBASEiEB3802.
EcoGeneiEG14049. zinT.

Phylogenomic databases

eggNOGiENOG4108MEK. Bacteria.
COG3443. LUCA.
HOGENOMiHOG000219533.
InParanoidiP76344.
OMAiVQFSDHT.
PhylomeDBiP76344.

Enzyme and pathway databases

BioCyciEcoCyc:G7061-MONOMER.
ECOL316407:JW1956-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76344.
PROiP76344.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR015304. ZinT_dom.
[Graphical view]
PfamiPF09223. ZinT. 1 hit.
[Graphical view]
SUPFAMiSSF50814. SSF50814. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZINT_ECOLI
AccessioniPrimary (citable) accession number: P76344
Secondary accession number(s): Q2MAZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.