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Protein

Protein-methionine-sulfoxide reductase heme-binding subunit MsrQ

Gene

msrQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation, including the primary periplasmic chaperone SurA and the lipoprotein Pal. MsrQ provides electrons for reduction to the reductase catalytic subunit MsrP, using the quinone pool of the respiratory chain.1 Publication

Cofactori

Protein has several cofactor binding sites:

Redox potential

E0 is -8 +/-16 mV at pH 7 for heme b.1 Publication

GO - Molecular functioni

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • protein repair Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Flavoprotein, FMN, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7060-MONOMER.
ECOL316407:JW1955-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-methionine-sulfoxide reductase heme-binding subunit MsrQUniRule annotation1 Publication
Alternative name(s):
Flavocytochrome MsrQUniRule annotation
Gene namesi
Name:msrQ1 Publication
Synonyms:yedZImported
Ordered Locus Names:b1972, JW1955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14048. yedZ.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicCurated
Transmembranei10 – 3021HelicalUniRule annotationAdd
BLAST
Topological domaini31 – 4414PeriplasmicCuratedAdd
BLAST
Transmembranei45 – 6723HelicalUniRule annotationAdd
BLAST
Topological domaini68 – 8114CytoplasmicCuratedAdd
BLAST
Transmembranei82 – 10221HelicalUniRule annotationAdd
BLAST
Topological domaini103 – 11513PeriplasmicCuratedAdd
BLAST
Transmembranei116 – 13621HelicalUniRule annotationAdd
BLAST
Topological domaini137 – 14913CytoplasmicCuratedAdd
BLAST
Transmembranei150 – 16718HelicalUniRule annotationAdd
BLAST
Topological domaini168 – 1703PeriplasmicCurated
Transmembranei171 – 18919HelicalUniRule annotationAdd
BLAST
Topological domaini190 – 21122CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking the msrPQ genes display no visible growth defect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Protein-methionine-sulfoxide reductase heme-binding subunit MsrQPRO_0000091574Add
BLAST

Proteomic databases

PaxDbiP76343.
PRIDEiP76343.

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit (MsrP) and a heme-binding subunit (MsrQ).1 Publication

Protein-protein interaction databases

BioGridi4260772. 11 interactions.
IntActiP76343. 1 interaction.
STRINGi511145.b1972.

Structurei

3D structure databases

ProteinModelPortaliP76343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MsrQ family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DQ2. Bacteria.
COG2717. LUCA.
HOGENOMiHOG000255015.
InParanoidiP76343.
KOiK17247.
OMAiHFFWMRA.
OrthoDBiEOG6N3CSP.
PhylomeDBiP76343.

Family and domain databases

HAMAPiMF_01207. MsrQ.
InterProiIPR013130. Fe3_Rdtase_TM_dom.
IPR022837. MsrQ.
[Graphical view]
PfamiPF01794. Ferric_reduct. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLTAKQVTW LKVCLHLAGL LPFLWLVWAI NHGGLGADPV KDIQHFTGRT
60 70 80 90 100
ALKFLLATLL ITPLARYAKQ PLLIRTRRLL GLWCFAWATL HLTSYALLEL
110 120 130 140 150
GVNNLALLGK ELITRPYLTL GIISWVILLA LAFTSTQAMQ RKLGKHWQQL
160 170 180 190 200
HNFVYLVAIL APIHYLWSVK IISPQPLIYA GLAVLLLALR YKKLRSLFNR
210
LRKQVHNKLS V
Length:211
Mass (Da):24,068
Last modified:February 1, 1997 - v1
Checksum:i4BB07AFF858D69BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75038.1.
AP009048 Genomic DNA. Translation: BAE76560.1.
PIRiH64961.
RefSeqiNP_416481.1. NC_000913.3.
WP_001240091.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75038; AAC75038; b1972.
BAE76560; BAE76560; BAE76560.
GeneIDi946483.
KEGGiecj:JW1955.
eco:b1972.
PATRICi32119275. VBIEscCol129921_2052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75038.1.
AP009048 Genomic DNA. Translation: BAE76560.1.
PIRiH64961.
RefSeqiNP_416481.1. NC_000913.3.
WP_001240091.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260772. 11 interactions.
IntActiP76343. 1 interaction.
STRINGi511145.b1972.

Proteomic databases

PaxDbiP76343.
PRIDEiP76343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75038; AAC75038; b1972.
BAE76560; BAE76560; BAE76560.
GeneIDi946483.
KEGGiecj:JW1955.
eco:b1972.
PATRICi32119275. VBIEscCol129921_2052.

Organism-specific databases

EchoBASEiEB3801.
EcoGeneiEG14048. yedZ.

Phylogenomic databases

eggNOGiENOG4105DQ2. Bacteria.
COG2717. LUCA.
HOGENOMiHOG000255015.
InParanoidiP76343.
KOiK17247.
OMAiHFFWMRA.
OrthoDBiEOG6N3CSP.
PhylomeDBiP76343.

Enzyme and pathway databases

BioCyciEcoCyc:G7060-MONOMER.
ECOL316407:JW1955-MONOMER.

Miscellaneous databases

PROiP76343.

Family and domain databases

HAMAPiMF_01207. MsrQ.
InterProiIPR013130. Fe3_Rdtase_TM_dom.
IPR022837. MsrQ.
[Graphical view]
PfamiPF01794. Ferric_reduct. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis."
    Drew D., Sjoestrand D., Nilsson J., Urbig T., Chin C.-N., de Gier J.-W., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 99:2690-2695(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  4. "Characterization of an Escherichia coli sulfite oxidase homologue reveals the role of a conserved active site cysteine in assembly and function."
    Brokx S.J., Rothery R.A., Zhang G., Ng D.P., Weiner J.H.
    Biochemistry 44:10339-10348(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, DISRUPTION PHENOTYPE, EPR SPECTROSCOPY, REDOX POTENTIAL, INTERACTION WITH MSRP, SUBUNIT.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "A scalable, GFP-based pipeline for membrane protein overexpression screening and purification."
    Drew D., Slotboom D.J., Friso G., Reda T., Genevaux P., Rapp M., Meindl-Beinker N.M., Lambert W., Lerch M., Daley D.O., Van Wijk K.J., Hirst J., Kunji E., De Gier J.W.
    Protein Sci. 14:2011-2017(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, FMN-BINDING.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons."
    Gennaris A., Ezraty B., Henry C., Agrebi R., Vergnes A., Oheix E., Bos J., Leverrier P., Espinosa L., Szewczyk J., Vertommen D., Iranzo O., Collet J.F., Barras F.
    Nature 528:409-412(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.

Entry informationi

Entry nameiMSRQ_ECOLI
AccessioniPrimary (citable) accession number: P76343
Secondary accession number(s): Q2MAZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.