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Protein

Probable diguanylate cyclase YedQ

Gene

yedQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of cellulose production. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361MagnesiumBy similarity
Sitei441 – 4411Transition state stabilizerSequence analysis
Binding sitei444 – 4441SubstrateBy similarity
Binding sitei453 – 4531SubstrateBy similarity
Active sitei479 – 4791Proton acceptorSequence analysis
Metal bindingi479 – 4791MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellulose biosynthetic process Source: UniProtKB-KW
  • negative regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cellulose biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7049-MONOMER.
ECOL316407:JW5832-MONOMER.
UniPathwayiUPA00599.
UPA00694.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase YedQ (EC:2.7.7.65)
Short name:
DGC
Alternative name(s):
Cellulose synthesis regulatory protein
Gene namesi
Name:yedQ
Ordered Locus Names:b1956, JW5832
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14040. yedQ.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4021HelicalSequence analysisAdd
BLAST
Transmembranei360 – 38021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption partially suppresses the reduced motility of a yhjH disruption; concomitant disruption of dosC, yegE, yedQ and yfiN completely restores motility, suggesting these 4 genes, together with the c-di-GMP phosphodiesterase YhjH, form a network that regulates cell motility by altering levels of c-di-GMP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Probable diguanylate cyclase YedQPRO_0000169097Add
BLAST

Proteomic databases

PaxDbiP76330.
PRIDEiP76330.

Expressioni

Inductioni

Constitutively expressed at both 28 and 37 degrees Celsius. Induced by 0.3 M NaCl. Expression is RpoS dependent.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4261052. 3 interactions.
STRINGi511145.b1956.

Structurei

3D structure databases

ProteinModelPortaliP76330.
SMRiP76330. Positions 401-558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini428 – 563136GGDEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GGDEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000121343.
OMAiEAYVANQ.
OrthoDBiEOG6G4VQG.

Family and domain databases

InterProiIPR033416. CHASE7.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF17151. CHASE7. 1 hit.
PF00990. GGDEF. 1 hit.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHETKMENQ SWLKKLARRL GPGHVVNLCF IVVLLFSTLL TWREVVVLED
60 70 80 90 100
AYISSQRNHL ENVANALDKH LQYNVDKLIF LRNGMREALV APLDFTSLRD
110 120 130 140 150
AVTEFEQHRD EHAWKIELNR RRTLPVNGVS DALVSEGNLL SRENESLDNE
160 170 180 190 200
ITAALEVGYL LRLAHNSSSM VEQAMYVSRA GFYVSTQPTL FTRNVPTRYY
210 220 230 240 250
GYVTQPWFIG HSQRENRHRA VRWFTSQPEH ASNTEPQVTV SVPVDSNNYW
260 270 280 290 300
YGVLGMSIPV RTMQQFLRNA IDKNLDGEYQ LYDSKLRFLT SSNPDHPTGN
310 320 330 340 350
IFDPRELALL AQAMEHDTRG GIRMDSRYVS WERLDHFDGV LVRVHTLSEG
360 370 380 390 400
VRGDFGSISI ALTLLWALFT TMLLISWYVI RRMVSNMYVL QSSLQWQAWH
410 420 430 440 450
DTLTRLYNRG ALFEKARPLA KLCQTHQHPF SVIQVDLDHF KAINDRFGHQ
460 470 480 490 500
AGDRVLSHAA GLISSSLRAQ DVAGRVGGEE FCVILPGASL TEAAEVAERI
510 520 530 540 550
RLKLNEKEML IAKSTTIRIS ASLGVSSSEE TGDYDFEQLQ SLADRRLYLA
560
KQAGRNRVFA SDNA
Length:564
Mass (Da):64,283
Last modified:March 5, 2002 - v2
Checksum:i05FB02C1BE2A8938
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75022.2.
AP009048 Genomic DNA. Translation: BAA15784.1.
PIRiH64959.
RefSeqiNP_416465.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75022; AAC75022; b1956.
BAA15784; BAA15784; BAA15784.
GeneIDi946471.
KEGGiecj:JW5832.
eco:b1956.
PATRICi32119239. VBIEscCol129921_2034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75022.2.
AP009048 Genomic DNA. Translation: BAA15784.1.
PIRiH64959.
RefSeqiNP_416465.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP76330.
SMRiP76330. Positions 401-558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261052. 3 interactions.
STRINGi511145.b1956.

Proteomic databases

PaxDbiP76330.
PRIDEiP76330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75022; AAC75022; b1956.
BAA15784; BAA15784; BAA15784.
GeneIDi946471.
KEGGiecj:JW5832.
eco:b1956.
PATRICi32119239. VBIEscCol129921_2034.

Organism-specific databases

EchoBASEiEB3794.
EcoGeneiEG14040. yedQ.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000121343.
OMAiEAYVANQ.
OrthoDBiEOG6G4VQG.

Enzyme and pathway databases

UniPathwayiUPA00599.
UPA00694.
BioCyciEcoCyc:G7049-MONOMER.
ECOL316407:JW5832-MONOMER.

Miscellaneous databases

PROiP76330.

Family and domain databases

InterProiIPR033416. CHASE7.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF17151. CHASE7. 1 hit.
PF00990. GGDEF. 1 hit.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli."
    Da Re S., Ghigo J.-M.
    J. Bacteriol. 188:3073-3087(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION PATHWAY.
    Strain: 1094.
  5. "Cyclic-di-GMP-mediated signalling within the sigma network of Escherichia coli."
    Weber H., Pesavento C., Possling A., Tischendorf G., Hengge R.
    Mol. Microbiol. 62:1014-1034(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, RPOS-DEPENDENCE.
    Strain: K12 / MC4100.
  6. "Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli."
    Sommerfeldt N., Possling A., Becker G., Pesavento C., Tschowri N., Hengge R.
    Microbiology 155:1318-1331(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, RPOS-DEPENDENCE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Second messenger-mediated adjustment of bacterial swimming velocity."
    Boehm A., Kaiser M., Li H., Spangler C., Kasper C.A., Ackermann M., Kaever V., Sourjik V., Roth V., Jenal U.
    Cell 141:107-116(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN MOTILITY, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiYEDQ_ECOLI
AccessioniPrimary (citable) accession number: P76330
Secondary accession number(s): P94746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: May 11, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.