Reviewed, UniProtKB/Swiss-Prot P76329 (MPGP_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative mannosyl-3-phosphoglycerate phosphatase
      Short name=MPGP
    EC=3.1.3.70

Gene names

Name:	yedP
Ordered Locus Names:	b1955, JW1938

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2(alpha-D-mannosyl)-3-phosphoglycerate + H2O = 2(alpha-D-mannosyl)-D-glycerate + phosphate. HAMAP MF_00617
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MPGP family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannosyl-3-phosphoglycerate phosphatase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yedI	P46125	1	EBI-1126577,EBI-1126588

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 271

271

Putative mannosyl-3-phosphoglycerate phosphatase HAMAP MF_00617

PRO_0000184974

Secondary structure

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271

Helix Strand Turn

Details...

Beta strand

8 – 13

6

Turn

15 – 17

3

Helix

30 – 38

9

Beta strand

43 – 46

4

Helix

51 – 60

10

Beta strand

68 – 70

3

Helix

71 – 73

3

Beta strand

75 – 77

3

Turn

86 – 89

4

Beta strand

90 – 92

3

Helix

97 – 111

15

Beta strand

115 – 117

3

Helix

118 – 120

3

Helix

123 – 130

8

Helix

134 – 141

8

Beta strand

147 – 151

5

Helix

155 – 167

13

Beta strand

170 – 174

5

Beta strand

179 – 183

5

Helix

188 – 203

16

Beta strand

208 – 215

8

Helix

216 – 218

3

Helix

219 – 223

5

Beta strand

225 – 233

9

Sequences

	Sequence		Length	Mass (Da)
	P76329-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1192A5DC83F71816	FASTA	271	30,439
	10 20 30 40 50 60 MFSIQQPLLV FSDLDGTLLD SHSYDWQPAA PWLTRLREAN VPVILCSSKT SAEMLYLQKT 70 80 90 100 110 120 LGLQGLPLIA ENGAVIQLAE QWQEIDGFPR IISGISHGEI SLVLNTLREK EHFKFTTFDD 130 140 150 160 170 180 VDDATIAEWT GLSRSQAALT QLHEASVTLI WRDSDERMAQ FTARLNELGL QFMQGARFWH 190 200 210 220 230 240 VLDASAGKDQ AANWIIATYQ QLSGKRPTTL GLGDGPNDAP LLEVMDYAVI VKGLNREGVH 250 260 270 LHDEDPARVW RTQREGPEGW REGLDHFFSA R

References

[1]

"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.

[2]

"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U00096 Genomic DNA. Translation: AAC75021.1.
AP009048 Genomic DNA. Translation: BAE76555.1.

PIR

G64959.

RefSeq

AP_002566.1.
NP_416464.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XVI	X-ray	2.26	A/B	1-271	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P76329. 3 interactions.

Genome annotation databases

GeneID

946472.

GenomeReviews

Gene locus JW1938 in contig AP009048_GR.
Gene locus b1955 in contig U00096_GR.

KEGG

ecj:JW1938.
eco:b1955.

Organism-specific databases

EchoBASE

EB3793.

EcoGene

EG14039. yedP.

CMR

Search...

Phylogenomic databases

HOGENOM

P76329.

OMA

P76329. LGDSPND.

Enzyme and pathway databases

BioCyc

EcoCyc:G7048-MON.

Family and domain databases

HAMAP

MF_00617.
[Tree]

InterPro

IPR013200. HAD-SF_hydro-like_3.
IPR006379. HAD-SF_hydro_IIB.
IPR006381. HAD-SF_hydro_IIB_YedP.
IPR012815. MPG_phos_rel.
[Graphical view]

Pfam

PF08282. Hydrolase_3. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01484. HAD-SF-IIB. 1 hit.
TIGR01486. HAD-SF-IIB-MPGP. 1 hit.
TIGR02463. MPGP_rel. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MPGP_ECOLI

Accession

Primary (citable) accession number: P76329
Secondary accession number(s): Q2MB01

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)