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P76316

- DCYD_ECOLI

UniProt

P76316 - DCYD_ECOLI

Protein

D-cysteine desulfhydrase

Gene

dcyD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine. Can also catalyze the degradation of 3-chloro-D-alanine.1 PublicationUniRule annotation

    Catalytic activityi

    D-cysteine + H2O = H2S + NH3 + pyruvate.1 PublicationUniRule annotation

    Cofactori

    Pyridoxal phosphate.1 PublicationUniRule annotation

    pH dependencei

    Optimum pH is 9.0.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. 3-chloro-D-alanine dehydrochlorinase activity Source: EcoCyc
    2. D-cysteine desulfhydrase activity Source: EcoCyc
    3. pyridoxal phosphate binding Source: EcoCyc

    GO - Biological processi

    1. cellular response to sulfur starvation Source: EcoCyc
    2. D-amino acid metabolic process Source: EcoliWiki
    3. D-cysteine catabolic process Source: EcoCyc
    4. sulfur compound metabolic process Source: EcoliWiki
    5. sulfur utilization Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:DCYSDESULF-MONOMER.
    ECOL316407:JW5313-MONOMER.
    MetaCyc:DCYSDESULF-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-cysteine desulfhydraseUniRule annotation (EC:4.4.1.15UniRule annotation)
    Gene namesi
    Name:dcyDUniRule annotation
    Synonyms:yedO
    Ordered Locus Names:b1919, JW5313
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14038. dcyD.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 328327D-cysteine desulfhydrasePRO_0000184513Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei51 – 511N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PaxDbiP76316.
    PRIDEiP76316.

    Expressioni

    Inductioni

    By sulfate starvation.1 Publication

    Gene expression databases

    GenevestigatoriP76316.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    nanEP0A7611EBI-562060,EBI-561432

    Protein-protein interaction databases

    DIPiDIP-11847N.
    IntActiP76316. 9 interactions.
    STRINGi511145.b1919.

    Structurei

    3D structure databases

    ProteinModelPortaliP76316.
    SMRiP76316. Positions 7-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ACC deaminase/D-cysteine desulfhydrase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2515.
    HOGENOMiHOG000022459.
    KOiK05396.
    OMAiPYLVPIG.
    OrthoDBiEOG6FBX0P.
    PhylomeDBiP76316.

    Family and domain databases

    HAMAPiMF_01045. D_Cys_desulfhydr.
    InterProiIPR027278. ACCD_DCysDesulf.
    IPR005966. D-Cys_desShydrase.
    IPR023702. D_Cys_desulphydr_bac.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006278. ACCD_DCysDesulf. 1 hit.
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01275. ACC_deam_rel. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P76316-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLHNLTRFP RLEFIGAPTP LEYLPRFSDY LGREIFIKRD DVTPMAMGGN    50
    KLRKLEFLAA DALREGADTL ITAGAIQSNH VRQTAAVAAK LGLHCVALLE 100
    NPIGTTAENY LTNGNRLLLD LFNTQIEMCD ALTDPNAQLE ELATRVEAQG 150
    FRPYVIPVGG SNALGALGYV ESALEIAQQC EGAVNISSVV VASGSAGTHA 200
    GLAVGLEHLM PESELIGVTV SRSVADQLPK VVNLQQAIAK ELELTASAEI 250
    LLWDDYFAPG YGVPNDEGME AVKLLARLEG ILLDPVYTGK AMAGLIDGIS 300
    QKRFKDEGPI LFIHTGGAPA LFAYHPHV 328
    Length:328
    Mass (Da):35,153
    Last modified:January 23, 2007 - v4
    Checksum:i4179DE645C0B32D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74986.2.
    AP009048 Genomic DNA. Translation: BAA15739.2.
    PIRiD64955.
    RefSeqiNP_416429.4. NC_000913.3.
    YP_490176.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74986; AAC74986; b1919.
    BAA15739; BAA15739; BAA15739.
    GeneIDi12931758.
    946831.
    KEGGiecj:Y75_p1890.
    eco:b1919.
    PATRICi32119173. VBIEscCol129921_2001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74986.2 .
    AP009048 Genomic DNA. Translation: BAA15739.2 .
    PIRi D64955.
    RefSeqi NP_416429.4. NC_000913.3.
    YP_490176.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76316.
    SMRi P76316. Positions 7-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11847N.
    IntActi P76316. 9 interactions.
    STRINGi 511145.b1919.

    Proteomic databases

    PaxDbi P76316.
    PRIDEi P76316.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74986 ; AAC74986 ; b1919 .
    BAA15739 ; BAA15739 ; BAA15739 .
    GeneIDi 12931758.
    946831.
    KEGGi ecj:Y75_p1890.
    eco:b1919.
    PATRICi 32119173. VBIEscCol129921_2001.

    Organism-specific databases

    EchoBASEi EB3792.
    EcoGenei EG14038. dcyD.

    Phylogenomic databases

    eggNOGi COG2515.
    HOGENOMi HOG000022459.
    KOi K05396.
    OMAi PYLVPIG.
    OrthoDBi EOG6FBX0P.
    PhylomeDBi P76316.

    Enzyme and pathway databases

    BioCyci EcoCyc:DCYSDESULF-MONOMER.
    ECOL316407:JW5313-MONOMER.
    MetaCyc:DCYSDESULF-MONOMER.

    Miscellaneous databases

    PROi P76316.

    Gene expression databases

    Genevestigatori P76316.

    Family and domain databases

    HAMAPi MF_01045. D_Cys_desulfhydr.
    InterProi IPR027278. ACCD_DCysDesulf.
    IPR005966. D-Cys_desShydrase.
    IPR023702. D_Cys_desulphydr_bac.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view ]
    Pfami PF00291. PALP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006278. ACCD_DCysDesulf. 1 hit.
    SUPFAMi SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR01275. ACC_deam_rel. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Role of D-cysteine desulfhydrase in the adaptation of Escherichia coli to D-cysteine."
      Soutourina J., Blanquet S., Plateau P.
      J. Biol. Chem. 276:40864-40872(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION, INDUCTION.
      Strain: K37.
    5. "D-cysteine desulfhydrase of Escherichia coli. Purification and characterization."
      Nagasawa T., Ishii T., Kumagai H., Yamada H.
      Eur. J. Biochem. 153:541-551(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiDCYD_ECOLI
    AccessioniPrimary (citable) accession number: P76316
    Secondary accession number(s): O08478, O08479
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3