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Protein

D-cysteine desulfhydrase

Gene

dcyD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine. Can also catalyze the degradation of 3-chloro-D-alanine.UniRule annotation1 Publication

Catalytic activityi

D-cysteine + H2O = H2S + NH3 + pyruvate.UniRule annotation1 Publication

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius.1 Publication

GO - Molecular functioni

  1. 3-chloro-D-alanine dehydrochlorinase activity Source: EcoCyc
  2. D-cysteine desulfhydrase activity Source: EcoliWiki
  3. pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

  1. cellular response to sulfur starvation Source: EcoCyc
  2. D-amino acid metabolic process Source: EcoliWiki
  3. D-cysteine catabolic process Source: EcoCyc
  4. sulfur compound metabolic process Source: EcoliWiki
  5. sulfur utilization Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:DCYSDESULF-MONOMER.
ECOL316407:JW5313-MONOMER.
MetaCyc:DCYSDESULF-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-cysteine desulfhydraseUniRule annotation (EC:4.4.1.15UniRule annotation)
Gene namesi
Name:dcyDUniRule annotation
Synonyms:yedO
Ordered Locus Names:b1919, JW5313
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14038. dcyD.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 328327D-cysteine desulfhydrasePRO_0000184513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PaxDbiP76316.
PRIDEiP76316.

Expressioni

Inductioni

By sulfate starvation.1 Publication

Gene expression databases

GenevestigatoriP76316.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
nanEP0A7611EBI-562060,EBI-561432

Protein-protein interaction databases

DIPiDIP-11847N.
IntActiP76316. 9 interactions.
STRINGi511145.b1919.

Structurei

3D structure databases

ProteinModelPortaliP76316.
SMRiP76316. Positions 7-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ACC deaminase/D-cysteine desulfhydrase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2515.
HOGENOMiHOG000022459.
InParanoidiP76316.
KOiK05396.
OMAiWVPHEDA.
OrthoDBiEOG6FBX0P.
PhylomeDBiP76316.

Family and domain databases

HAMAPiMF_01045. D_Cys_desulfhydr.
InterProiIPR027278. ACCD_DCysDesulf.
IPR005966. D-Cys_desShydrase.
IPR023702. D_Cys_desulphydr_bac.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF006278. ACCD_DCysDesulf. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01275. ACC_deam_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLHNLTRFP RLEFIGAPTP LEYLPRFSDY LGREIFIKRD DVTPMAMGGN
60 70 80 90 100
KLRKLEFLAA DALREGADTL ITAGAIQSNH VRQTAAVAAK LGLHCVALLE
110 120 130 140 150
NPIGTTAENY LTNGNRLLLD LFNTQIEMCD ALTDPNAQLE ELATRVEAQG
160 170 180 190 200
FRPYVIPVGG SNALGALGYV ESALEIAQQC EGAVNISSVV VASGSAGTHA
210 220 230 240 250
GLAVGLEHLM PESELIGVTV SRSVADQLPK VVNLQQAIAK ELELTASAEI
260 270 280 290 300
LLWDDYFAPG YGVPNDEGME AVKLLARLEG ILLDPVYTGK AMAGLIDGIS
310 320
QKRFKDEGPI LFIHTGGAPA LFAYHPHV
Length:328
Mass (Da):35,153
Last modified:January 23, 2007 - v4
Checksum:i4179DE645C0B32D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74986.2.
AP009048 Genomic DNA. Translation: BAA15739.2.
PIRiD64955.
RefSeqiNP_416429.4. NC_000913.3.
YP_490176.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74986; AAC74986; b1919.
BAA15739; BAA15739; BAA15739.
GeneIDi12931758.
946831.
KEGGiecj:Y75_p1890.
eco:b1919.
PATRICi32119173. VBIEscCol129921_2001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74986.2.
AP009048 Genomic DNA. Translation: BAA15739.2.
PIRiD64955.
RefSeqiNP_416429.4. NC_000913.3.
YP_490176.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP76316.
SMRiP76316. Positions 7-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-11847N.
IntActiP76316. 9 interactions.
STRINGi511145.b1919.

Proteomic databases

PaxDbiP76316.
PRIDEiP76316.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74986; AAC74986; b1919.
BAA15739; BAA15739; BAA15739.
GeneIDi12931758.
946831.
KEGGiecj:Y75_p1890.
eco:b1919.
PATRICi32119173. VBIEscCol129921_2001.

Organism-specific databases

EchoBASEiEB3792.
EcoGeneiEG14038. dcyD.

Phylogenomic databases

eggNOGiCOG2515.
HOGENOMiHOG000022459.
InParanoidiP76316.
KOiK05396.
OMAiWVPHEDA.
OrthoDBiEOG6FBX0P.
PhylomeDBiP76316.

Enzyme and pathway databases

BioCyciEcoCyc:DCYSDESULF-MONOMER.
ECOL316407:JW5313-MONOMER.
MetaCyc:DCYSDESULF-MONOMER.

Miscellaneous databases

PROiP76316.

Gene expression databases

GenevestigatoriP76316.

Family and domain databases

HAMAPiMF_01045. D_Cys_desulfhydr.
InterProiIPR027278. ACCD_DCysDesulf.
IPR005966. D-Cys_desShydrase.
IPR023702. D_Cys_desulphydr_bac.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF006278. ACCD_DCysDesulf. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01275. ACC_deam_rel. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Role of D-cysteine desulfhydrase in the adaptation of Escherichia coli to D-cysteine."
    Soutourina J., Blanquet S., Plateau P.
    J. Biol. Chem. 276:40864-40872(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION, INDUCTION.
    Strain: K37.
  5. "D-cysteine desulfhydrase of Escherichia coli. Purification and characterization."
    Nagasawa T., Ishii T., Kumagai H., Yamada H.
    Eur. J. Biochem. 153:541-551(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiDCYD_ECOLI
AccessioniPrimary (citable) accession number: P76316
Secondary accession number(s): O08478, O08479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.