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P76316 (DCYD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-cysteine desulfhydrase

EC=4.4.1.15
Gene names
Name:dcyD
Synonyms:yedO
Ordered Locus Names:b1919, JW5313
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine. Can also catalyze the degradation of 3-chloro-D-alanine. Ref.5

Catalytic activity

D-cysteine + H2O = H2S + NH3 + pyruvate. Ref.5

Cofactor

Pyridoxal phosphate. Ref.5

Subunit structure

Homodimer Probable. Ref.5

Induction

By sulfate starvation. Ref.4

Sequence similarities

Belongs to the ACC deaminase/D-cysteine desulfhydrase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.0. Ref.5

Temperature dependence:

Optimum temperature is 45 degrees Celsius.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

nanEP0A7611EBI-562060,EBI-561432

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 328327D-cysteine desulfhydrase HAMAP-Rule MF_01045
PRO_0000184513

Amino acid modifications

Modified residue511N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P76316 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 4179DE645C0B32D8

FASTA32835,153
        10         20         30         40         50         60 
MPLHNLTRFP RLEFIGAPTP LEYLPRFSDY LGREIFIKRD DVTPMAMGGN KLRKLEFLAA 

        70         80         90        100        110        120 
DALREGADTL ITAGAIQSNH VRQTAAVAAK LGLHCVALLE NPIGTTAENY LTNGNRLLLD 

       130        140        150        160        170        180 
LFNTQIEMCD ALTDPNAQLE ELATRVEAQG FRPYVIPVGG SNALGALGYV ESALEIAQQC 

       190        200        210        220        230        240 
EGAVNISSVV VASGSAGTHA GLAVGLEHLM PESELIGVTV SRSVADQLPK VVNLQQAIAK 

       250        260        270        280        290        300 
ELELTASAEI LLWDDYFAPG YGVPNDEGME AVKLLARLEG ILLDPVYTGK AMAGLIDGIS 

       310        320 
QKRFKDEGPI LFIHTGGAPA LFAYHPHV 

« Hide

References

« Hide 'large scale' references
[1]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Role of D-cysteine desulfhydrase in the adaptation of Escherichia coli to D-cysteine."
Soutourina J., Blanquet S., Plateau P.
J. Biol. Chem. 276:40864-40872(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION, INDUCTION.
Strain: K37.
[5]"D-cysteine desulfhydrase of Escherichia coli. Purification and characterization."
Nagasawa T., Ishii T., Kumagai H., Yamada H.
Eur. J. Biochem. 153:541-551(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74986.2.
AP009048 Genomic DNA. Translation: BAA15739.2.
PIRD64955.
RefSeqNP_416429.4. NC_000913.3.
YP_490176.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76316.
SMRP76316. Positions 7-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-11847N.
IntActP76316. 9 interactions.
STRING511145.b1919.

Proteomic databases

PaxDbP76316.
PRIDEP76316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74986; AAC74986; b1919.
BAA15739; BAA15739; BAA15739.
GeneID12931758.
946831.
KEGGecj:Y75_p1890.
eco:b1919.
PATRIC32119173. VBIEscCol129921_2001.

Organism-specific databases

EchoBASEEB3792.
EcoGeneEG14038. dcyD.

Phylogenomic databases

eggNOGCOG2515.
HOGENOMHOG000022459.
KOK05396.
OMAPYLVPIG.
OrthoDBEOG6FBX0P.
PhylomeDBP76316.

Enzyme and pathway databases

BioCycEcoCyc:DCYSDESULF-MONOMER.
ECOL316407:JW5313-MONOMER.
MetaCyc:DCYSDESULF-MONOMER.

Gene expression databases

GenevestigatorP76316.

Family and domain databases

HAMAPMF_01045. D_Cys_desulfhydr.
InterProIPR027278. ACCD_DCysDesulf.
IPR005966. D-Cys_desShydrase.
IPR023702. D_Cys_desulphydr_bac.
IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
[Graphical view]
PfamPF00291. PALP. 1 hit.
[Graphical view]
PIRSFPIRSF006278. ACCD_DCysDesulf. 1 hit.
SUPFAMSSF53686. SSF53686. 1 hit.
TIGRFAMsTIGR01275. ACC_deam_rel. 1 hit.
ProtoNetSearch...

Other

PROP76316.

Entry information

Entry nameDCYD_ECOLI
AccessionPrimary (citable) accession number: P76316
Secondary accession number(s): O08478, O08479
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 114 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene