P76316 (DCYD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-cysteine desulfhydrase
EC=4.4.1.15

Gene names

Name:	dcyD
Synonyms:	yedO
Ordered Locus Names:	b1919, JW5313

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine. Can also catalyze the degradation of 3-chloro-D-alanine. HAMAP MF_01045
Catalytic activity	D-cysteine + H2O = H2S + NH3 + pyruvate. HAMAP MF_01045
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer Probable.
Induction	By sulfate starvation. HAMAP MF_01045
Sequence similarities	Belongs to the ACC deaminase/D-cysteine desulfhydrase family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0. HAMAP MF_01045 Temperature dependence: Optimum temperature is 45 degrees Celsius.

Ontologies

Keywords
Biological process	Stress response
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	D-amino acid metabolic process Inferred from direct assay Ref.4. Source: EcoliWiki response to stress Inferred from mutant phenotype Ref.4. Source: EcoliWiki sulfur compound metabolic process Inferred from direct assay Ref.4. Source: EcoliWiki
Molecular function	D-cysteine desulfhydrase activity Inferred from direct assay Ref.4. Source: EcoliWiki pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nanE	P0A761	1	EBI-562060,EBI-561432

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 328	327	D-cysteine desulfhydrase HAMAP MF_01045		PRO_0000184513
Amino acid modifications
	Modified residue	51	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P76316 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 4179DE645C0B32D8	FASTA	328	35,153
	10 20 30 40 50 60 MPLHNLTRFP RLEFIGAPTP LEYLPRFSDY LGREIFIKRD DVTPMAMGGN KLRKLEFLAA 70 80 90 100 110 120 DALREGADTL ITAGAIQSNH VRQTAAVAAK LGLHCVALLE NPIGTTAENY LTNGNRLLLD 130 140 150 160 170 180 LFNTQIEMCD ALTDPNAQLE ELATRVEAQG FRPYVIPVGG SNALGALGYV ESALEIAQQC 190 200 210 220 230 240 EGAVNISSVV VASGSAGTHA GLAVGLEHLM PESELIGVTV SRSVADQLPK VVNLQQAIAK 250 260 270 280 290 300 ELELTASAEI LLWDDYFAPG YGVPNDEGME AVKLLARLEG ILLDPVYTGK AMAGLIDGIS 310 320 QKRFKDEGPI LFIHTGGAPA LFAYHPHV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Role of D-cysteine desulfhydrase in the adaptation of Escherichia coli to D-cysteine." Soutourina J., Blanquet S., Plateau P. J. Biol. Chem. 276:40864-40872(2001) [PubMed: 11527960] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION. Strain: K37.
[5]	"D-cysteine desulfhydrase of Escherichia coli. Purification and characterization." Nagasawa T., Ishii T., Kumagai H., Yamada H. Eur. J. Biochem. 153:541-551(1985) [PubMed: 3908101] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74986.2. AP009048 Genomic DNA. Translation: BAA15739.2.
PIR	D64955.
RefSeq	NP_416429.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P76316.
SMR	P76316. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11847N.
IntAct	P76316. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002386; EBESCP00000002386; EBESCG00000001947. EBESCT00000014648; EBESCP00000013939; EBESCG00000013709.
GeneID	946831.
GenomeReviews	Gene locus JW5313 in contig AP009048_GR. Gene locus b1919 in contig U00096_GR.
KEGG	ecj:JW5313. eco:b1919.
PATRIC	32119173. VBIEscCol129921_2001.
Organism-specific databases
EchoBASE	EB3792.
EcoGene	EG14038. dcyD.
Phylogenomic databases
eggNOG	COG2515.
GeneTree	EBGT00050000011598.
HOGENOM	HBG689308.
OMA	YGEPNAG.
PhylomeDB	P76316.
ProtClustDB	PRK03910.
Enzyme and pathway databases
BioCyc	EcoCyc:DCYSDESULF-MONOMER. MetaCyc:DCYSDESULF-MONOMER.
Gene expression databases
Genevestigator	P76316.
Family and domain databases
HAMAP	MF_01045. D-Cys_desulfhydr. [Tree]
InterPro	IPR023702. D_Cys_desulphydr. IPR005966. PyrdxlP-dep_deaminase. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K05396.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01275. ACC_deam_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DCYD_ECOLI

Accession

Primary (citable) accession number: P76316
Secondary accession number(s): O08478, O08479

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 94 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program