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P76299 (FLHB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Flagellar biosynthetic protein FlhB
Gene names
Name:flhB
Synonyms:yecQ
Ordered Locus Names:b1880, JW1869
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the type III secretion exporter family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Flagellar biosynthetic protein FlhB
PRO_0000180949

Regions

Topological domain1 – 3131Periplasmic Potential
Transmembrane32 – 5221Helical; Potential
Topological domain53 – 7321Cytoplasmic Potential
Transmembrane74 – 9421Helical; Potential
Transmembrane95 – 11521Helical; Potential
Topological domain116 – 14429Cytoplasmic Potential
Transmembrane145 – 16521Helical; Potential
Topological domain166 – 18924Periplasmic Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 382172Cytoplasmic Potential

Experimental info

Sequence conflict2501A → R in AAC17834. Ref.1
Sequence conflict3161P → R in AAC17834. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P76299 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 3E319DFC30FEC4CC

FASTA38242,238
        10         20         30         40         50         60 
MSDESDDKTE APTPHRLEKA REEGQIPRSR ELTSLLILLV GVSVIWFGGV SLARRLSGML 

        70         80         90        100        110        120 
SAGLHFDHSI INDPNLILGQ IILLIREAML ALLPLISGVV LVALISPVML GGLVFSGKSL 

       130        140        150        160        170        180 
QPKFSKLNPL PGIKRMFSAQ TGAELLKAIL KTILVGSVTG FFLWHHWPQM MRLMAESPIT 

       190        200        210        220        230        240 
AMGNAMDLVG LCALLVVLGV IPMVGFDVFF QIFSHLKKLR MSRQDIRDEF KQSEGDPHVK 

       250        260        270        280        290        300 
GRIRQMQRAA ARRRMMADVP KADVIVNNPT HYSVALQYDE NKMSAPKVVA KGAGLVALRI 

       310        320        330        340        350        360 
REIGAENNVP TLEAPPLARA LYRHAEIGQQ IPGQLYAAVA EVLAWVWQLK RWRLAGGQRP 

       370        380 
VQPTHLPVPE ALDFINEKPT HE

« Hide

References

« Hide 'large scale' references
[1]"Genomic organization of flhB operon in E.coli."
Cho M., Matsumura P.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U88319 Genomic DNA. Translation: AAC17834.1.
U00096 Genomic DNA. Translation: AAC74950.1.
AP009048 Genomic DNA. Translation: BAA15696.1.
PIRH64950.
RefSeqNP_416394.1. NC_000913.2.
YP_490142.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76299.
SMRP76299. Positions 228-352.
ModBaseSearch...

Protein-protein interaction databases

IntActP76299. 3 interactions.
STRING511145.b1880.

Protein family/group databases

MEROPSN06.A01.

Proteomic databases

PRIDEP76299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74950; AAC74950; b1880.
BAA15696; BAA15696; BAA15696.
GeneID12931384.
946391.
KEGGecj:Y75_p1856.
eco:b1880.
PATRIC32119085. VBIEscCol129921_1960.

Organism-specific databases

EchoBASEEB3789.
EcoGeneEG14035. flhB.

Phylogenomic databases

eggNOGCOG1377.
HOGENOMHOG000253899.
KOK02401.
OMAEHYAVAV.
ProtClustDBPRK05702.

Enzyme and pathway databases

BioCycEcoCyc:G7028-MONOMER.
ECOL316407:JW1869-MONOMER.

Gene expression databases

GenevestigatorP76299.

Family and domain databases

InterProIPR006136. T3SS_FlhB.
IPR006135. T3SS_substrate_exporter.
[Graphical view]
PfamPF01312. Bac_export_2. 1 hit.
[Graphical view]
PRINTSPR00950. TYPE3IMSPROT.
TIGRFAMsTIGR00328. flhB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFLHB_ECOLI
AccessionPrimary (citable) accession number: P76299
Secondary accession number(s): O87955
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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