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Reviewed, UniProtKB/Swiss-Prot P76290 (CMOA_ECOLI)

Last modified February 9, 2010. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    tRNA (cmo5U34)-methyltransferase
    EC=2.1.1.-
Gene names
Name: cmoA
Synonyms: yecO
Ordered Locus Names: b1870, JW1859
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity. HAMAP MF_01589

Subunit structure

Monomer By similarity. HAMAP MF_01589

Sequence similarities

Belongs to the methyltransferase superfamily. CmoA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247tRNA (cmo5U34)-methyltransferase HAMAP MF_01589
PRO_0000169083

Regions

Region64 – 663S-adenosyl-L-methionine binding By similarity
Region117 – 1182S-adenosyl-L-methionine binding By similarity

Sites

Binding site391S-adenosyl-L-methionine By similarity
Binding site891S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1991Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
P76290-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 2930565435F5A7F4

FASTA24727,777
        10         20         30         40         50         60 
MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA ERFVQPGTQV 

        70         80         90        100        110        120 
YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR HIDAYKAPTP VDVIEGDIRD 

       130        140        150        160        170        180 
IAIENASMVV LNFTLQFLEP SERQALLDKI YQGLNPGGAL VLSEKFSFED AKVGELLFNM 

       190        200        210        220        230        240 
HHDFKRANGY SELEISQKRS MLENVMLTDS VETHKARLHN AGFEHSELWF QCFNFGSLVA 


LKAEDAA 

« Hide

References

[1]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74940.1.
AP009048 Genomic DNA. Translation: BAA15680.1.
PIRF64949.
RefSeqAP_002490.1.
NP_416384.1.

3D structure databases

SMRP76290. Positions 18-242.
ModBaseSearch...

Protein-protein interaction databases

STRINGP76290.

PTM databases

PhosSiteP76290.

2-D gel databases

SWISS-2DPAGEP76290.

Genome annotation databases

GeneID946380.
GenomeReviewsGene locus JW1859 in contig AP009048_GR.
Gene locus b1870 in contig U00096_GR.
KEGGecj:JW1859.
eco:b1870.

Organism-specific databases

EchoBASEEB3787.
EcoGeneEG14033. cmoA.
CMRSearch...

Phylogenomic databases

eggNOGCOG0500.
HOGENOMHBG296860.
OMANFTLQFI.

Enzyme and pathway databases

BioCycEcoCyc:G7020-MONOMER.
ECOL168927:B1870-MONOMER.

Gene expression databases

GenevestigatorP76290.

Family and domain databases

HAMAPMF_01589. tRNA_methyltr_CmoA.
[Tree]
InterProIPR013216. Methyltransf_11.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PfamPF08241. Methyltransf_11. 1 hit.
[Graphical view]
PIRSFPIRSF006325. MeTrfase_bac. 1 hit.
TIGRFAMsTIGR00740. Methyltrans_bac. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCMOA_ECOLI
AccessionPrimary (citable) accession number: P76290
Secondary accession number(s): O07982
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents