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Protein

Carboxy-S-adenosyl-L-methionine synthase

Gene

cmoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of S-adenosyl-L-methionine (SAM) to carboxy-S-adenosyl-L-methionine (Cx-SAM).UniRule annotation1 Publication

Catalytic activityi

Prephenate + S-adenosyl-L-methionine = phenylpyruvate + carboxy-S-adenosyl-L-methionine + H2O.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39S-adenosyl-L-methionineUniRule annotation2 Publications1
Binding sitei132S-adenosyl-L-methionineUniRule annotation2 Publications1
Binding sitei199S-adenosyl-L-methionineUniRule annotation2 Publications1

GO - Molecular functioni

GO - Biological processi

  • methylation Source: GO_Central
  • tRNA wobble uridine modification Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7020-MONOMER.
ECOL316407:JW1859-MONOMER.
MetaCyc:G7020-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-S-adenosyl-L-methionine synthaseUniRule annotationCurated (EC:2.1.3.-UniRule annotation1 Publication)
Short name:
Cx-SAM synthase1 PublicationUniRule annotation
Gene namesi
Name:cmoA2 PublicationsUniRule annotation
Synonyms:yecO
Ordered Locus Names:b1870, JW1859
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14033. cmoA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi89D → L: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690831 – 247Carboxy-S-adenosyl-L-methionine synthaseAdd BLAST247

Proteomic databases

PaxDbiP76290.
PRIDEiP76290.

2D gel databases

SWISS-2DPAGEP76290.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4263500. 11 interactors.
IntActiP76290. 6 interactors.
MINTiMINT-7290797.
STRINGi511145.b1870.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 35Combined sources16
Helixi39 – 53Combined sources15
Beta strandi59 – 64Combined sources6
Turni66 – 68Combined sources3
Helixi69 – 76Combined sources8
Beta strandi80 – 82Combined sources3
Beta strandi84 – 90Combined sources7
Helixi92 – 103Combined sources12
Beta strandi111 – 116Combined sources6
Turni118 – 120Combined sources3
Beta strandi125 – 133Combined sources9
Helixi135 – 137Combined sources3
Helixi140 – 153Combined sources14
Beta strandi154 – 166Combined sources13
Helixi171 – 187Combined sources17
Helixi190 – 192Combined sources3
Helixi196 – 203Combined sources8
Helixi211 – 221Combined sources11
Beta strandi224 – 232Combined sources9
Beta strandi235 – 241Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GEKX-ray1.50A/G1-247[»]
4IWNX-ray1.73A/B19-247[»]
ProteinModelPortaliP76290.
SMRiP76290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 66S-adenosyl-L-methionine bindingUniRule annotation2 Publications3
Regioni89 – 90S-adenosyl-L-methionine bindingUniRule annotation2 Publications2
Regioni117 – 118S-adenosyl-L-methionine bindingUniRule annotation2 Publications2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cx-SAM synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105DA6. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000218457.
InParanoidiP76290.
KOiK15256.
OMAiMIELYYL.
PhylomeDBiP76290.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01589. Cx_SAM_synthase. 1 hit.
InterProiIPR005271. CmoA.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00740. TIGR00740. 1 hit.

Sequencei

Sequence statusi: Complete.

P76290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA
60 70 80 90 100
ERFVQPGTQV YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR
110 120 130 140 150
HIDAYKAPTP VDVIEGDIRD IAIENASMVV LNFTLQFLEP SERQALLDKI
160 170 180 190 200
YQGLNPGGAL VLSEKFSFED AKVGELLFNM HHDFKRANGY SELEISQKRS
210 220 230 240
MLENVMLTDS VETHKARLHN AGFEHSELWF QCFNFGSLVA LKAEDAA
Length:247
Mass (Da):27,777
Last modified:February 1, 1997 - v1
Checksum:i2930565435F5A7F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74940.1.
AP009048 Genomic DNA. Translation: BAA15680.1.
PIRiF64949.
RefSeqiNP_416384.1. NC_000913.3.
WP_000019590.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74940; AAC74940; b1870.
BAA15680; BAA15680; BAA15680.
GeneIDi946380.
KEGGiecj:JW1859.
eco:b1870.
PATRICi32119063. VBIEscCol129921_1949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74940.1.
AP009048 Genomic DNA. Translation: BAA15680.1.
PIRiF64949.
RefSeqiNP_416384.1. NC_000913.3.
WP_000019590.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GEKX-ray1.50A/G1-247[»]
4IWNX-ray1.73A/B19-247[»]
ProteinModelPortaliP76290.
SMRiP76290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263500. 11 interactors.
IntActiP76290. 6 interactors.
MINTiMINT-7290797.
STRINGi511145.b1870.

2D gel databases

SWISS-2DPAGEP76290.

Proteomic databases

PaxDbiP76290.
PRIDEiP76290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74940; AAC74940; b1870.
BAA15680; BAA15680; BAA15680.
GeneIDi946380.
KEGGiecj:JW1859.
eco:b1870.
PATRICi32119063. VBIEscCol129921_1949.

Organism-specific databases

EchoBASEiEB3787.
EcoGeneiEG14033. cmoA.

Phylogenomic databases

eggNOGiENOG4105DA6. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000218457.
InParanoidiP76290.
KOiK15256.
OMAiMIELYYL.
PhylomeDBiP76290.

Enzyme and pathway databases

BioCyciEcoCyc:G7020-MONOMER.
ECOL316407:JW1859-MONOMER.
MetaCyc:G7020-MONOMER.

Miscellaneous databases

PROiP76290.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01589. Cx_SAM_synthase. 1 hit.
InterProiIPR005271. CmoA.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00740. TIGR00740. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCMOA_ECOLI
AccessioniPrimary (citable) accession number: P76290
Secondary accession number(s): O07982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mechanistic analyses show the involvement of a unique ylide intermediate as the carboxyl acceptor in the conversion of SAM to Cx-SAM.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.