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Protein

Carboxy-S-adenosyl-L-methionine synthase

Gene

cmoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of S-adenosyl-L-methionine (SAM) to carboxy-S-adenosyl-L-methionine (Cx-SAM).UniRule annotation1 Publication

Miscellaneous

Mechanistic analyses show the involvement of a unique ylide intermediate as the carboxyl acceptor in the conversion of SAM to Cx-SAM.1 Publication

Catalytic activityi

Prephenate + S-adenosyl-L-methionine = phenylpyruvate + carboxy-S-adenosyl-L-methionine + H2O.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39S-adenosyl-L-methionineUniRule annotation2 Publications1
Binding sitei132S-adenosyl-L-methionineUniRule annotation2 Publications1
Binding sitei199S-adenosyl-L-methionineUniRule annotation2 Publications1

GO - Molecular functioni

GO - Biological processi

  • tRNA wobble uridine modification Source: EcoCyc

Keywordsi

Molecular functionTransferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7020-MONOMER
MetaCyc:G7020-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-S-adenosyl-L-methionine synthaseUniRule annotationCurated (EC:2.1.3.-UniRule annotation1 Publication)
Short name:
Cx-SAM synthase1 PublicationUniRule annotation
Gene namesi
Name:cmoA2 PublicationsUniRule annotation
Synonyms:yecO
Ordered Locus Names:b1870, JW1859
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG14033 cmoA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi89D → L: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690831 – 247Carboxy-S-adenosyl-L-methionine synthaseAdd BLAST247

Proteomic databases

PaxDbiP76290
PRIDEiP76290

2D gel databases

SWISS-2DPAGEiP76290

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4263500, 34 interactors
IntActiP76290, 6 interactors
MINTiP76290
STRINGi316385.ECDH10B_2011

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 35Combined sources16
Helixi39 – 53Combined sources15
Beta strandi59 – 64Combined sources6
Turni66 – 68Combined sources3
Helixi69 – 76Combined sources8
Beta strandi80 – 82Combined sources3
Beta strandi84 – 90Combined sources7
Helixi92 – 103Combined sources12
Beta strandi111 – 116Combined sources6
Turni118 – 120Combined sources3
Beta strandi125 – 133Combined sources9
Helixi135 – 137Combined sources3
Helixi140 – 153Combined sources14
Beta strandi154 – 166Combined sources13
Helixi171 – 187Combined sources17
Helixi190 – 192Combined sources3
Helixi196 – 203Combined sources8
Helixi211 – 221Combined sources11
Beta strandi224 – 232Combined sources9
Beta strandi235 – 241Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GEKX-ray1.50A/G1-247[»]
4IWNX-ray1.73A/B19-247[»]
ProteinModelPortaliP76290
SMRiP76290
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 66S-adenosyl-L-methionine bindingUniRule annotation2 Publications3
Regioni89 – 90S-adenosyl-L-methionine bindingUniRule annotation2 Publications2
Regioni117 – 118S-adenosyl-L-methionine bindingUniRule annotation2 Publications2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cx-SAM synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105DA6 Bacteria
COG0500 LUCA
HOGENOMiHOG000218457
InParanoidiP76290
KOiK15256
OMAiMIELYYL
PhylomeDBiP76290

Family and domain databases

HAMAPiMF_01589 Cx_SAM_synthase, 1 hit
InterProiView protein in InterPro
IPR005271 CmoA
IPR029063 SAM-dependent_MTases
PANTHERiPTHR43861:SF2 PTHR43861:SF2, 1 hit
PIRSFiPIRSF006325 MeTrfase_bac, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00740 TIGR00740, 1 hit

Sequencei

Sequence statusi: Complete.

P76290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA
60 70 80 90 100
ERFVQPGTQV YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR
110 120 130 140 150
HIDAYKAPTP VDVIEGDIRD IAIENASMVV LNFTLQFLEP SERQALLDKI
160 170 180 190 200
YQGLNPGGAL VLSEKFSFED AKVGELLFNM HHDFKRANGY SELEISQKRS
210 220 230 240
MLENVMLTDS VETHKARLHN AGFEHSELWF QCFNFGSLVA LKAEDAA
Length:247
Mass (Da):27,777
Last modified:February 1, 1997 - v1
Checksum:i2930565435F5A7F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74940.1
AP009048 Genomic DNA Translation: BAA15680.1
PIRiF64949
RefSeqiNP_416384.1, NC_000913.3
WP_000019590.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74940; AAC74940; b1870
BAA15680; BAA15680; BAA15680
GeneIDi946380
KEGGiecj:JW1859
eco:b1870
PATRICifig|1411691.4.peg.378

Entry informationi

Entry nameiCMOA_ECOLI
AccessioniPrimary (citable) accession number: P76290
Secondary accession number(s): O07982
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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