Reviewed, UniProtKB/Swiss-Prot P76290 (CMOA_ECOLI)

Names and origin

Protein names

Recommended name:
    tRNA (cmo5U34)-methyltransferase
    EC=2.1.1.-

Gene names

Name:	cmoA
Synonyms:	yecO
Ordered Locus Names:	b1870, JW1859

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity. HAMAP MF_01589
Subunit structure	Monomer By similarity. HAMAP MF_01589
Sequence similarities	Belongs to the methyltransferase superfamily. CmoA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Molecular function	S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA (uracil) methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	tRNA (cmo5U34)-methyltransferase HAMAP MF_01589		PRO_0000169083
Regions
	Region	64 – 66	3	S-adenosyl-L-methionine binding By similarity
	Region	117 – 118	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	39	1	S-adenosyl-L-methionine By similarity
	Binding site	89	1	S-adenosyl-L-methionine By similarity
	Binding site	132	1	S-adenosyl-L-methionine By similarity
	Binding site	199	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P76290-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2930565435F5A7F4	FASTA	247	27,777
	10 20 30 40 50 60 MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA ERFVQPGTQV 70 80 90 100 110 120 YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR HIDAYKAPTP VDVIEGDIRD 130 140 150 160 170 180 IAIENASMVV LNFTLQFLEP SERQALLDKI YQGLNPGGAL VLSEKFSFED AKVGELLFNM 190 200 210 220 230 240 HHDFKRANGY SELEISQKRS MLENVMLTDS VETHKARLHN AGFEHSELWF QCFNFGSLVA LKAEDAA

References

[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74940.1. AP009048 Genomic DNA. Translation: BAA15680.1.
PIR	F64949.
RefSeq	AP_002490.1. NP_416384.1.
3D structure databases
SMR	P76290. Positions 18-242.
ModBase	Search...
Protein-protein interaction databases
STRING	P76290.
PTM databases
PhosSite	P76290.
2-D gel databases
SWISS-2DPAGE	P76290.
Genome annotation databases
GeneID	946380.
GenomeReviews	Gene locus JW1859 in contig AP009048_GR. Gene locus b1870 in contig U00096_GR.
KEGG	ecj:JW1859. eco:b1870.
Organism-specific databases
EchoBASE	EB3787.
EcoGene	EG14033. cmoA.
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG296860.
OMA	NFTLQFI.
Enzyme and pathway databases
BioCyc	EcoCyc:G7020-MONOMER. ECOL168927:B1870-MONOMER.
Gene expression databases
Genevestigator	P76290.
Family and domain databases
HAMAP	MF_01589. tRNA_methyltr_CmoA. [Tree]
InterPro	IPR013216. Methyltransf_11. IPR005271. tRNA_cmo5U34_MeTrfase. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
PIRSF	PIRSF006325. MeTrfase_bac. 1 hit.
TIGRFAMs	TIGR00740. Methyltrans_bac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CMOA_ECOLI

Accession

Primary (citable) accession number: P76290
Secondary accession number(s): O07982

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)