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Protein

Free methionine-R-sulfoxide reductase

Gene

msrC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation-reduction of the R-enantiomer of free methionine sulfoxide to methionine. Specific for free L-methionine-(R)-S-oxide.1 Publication

Catalytic activityi

L-methionine + thioredoxin disulfide + H2O = L-methionine (R)-S-oxide + thioredoxin.1 Publication

Kineticsi

  1. KM=3.9 mM for L-methionine-(R)-S-oxide1 Publication
  2. KM=9.8 µM for thioredoxin1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Enzyme and pathway databases

    BioCyciEcoCyc:G7005-MONOMER.
    ECOL316407:JW1821-MONOMER.
    MetaCyc:G7005-MONOMER.
    BRENDAi1.8.4.14. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Free methionine-R-sulfoxide reductase (EC:1.8.4.14)
    Short name:
    fRMsr
    Gene namesi
    Name:msrC
    Synonyms:yebR
    Ordered Locus Names:b1832, JW1821
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14020. msrC.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001715531 – 165Free methionine-R-sulfoxide reductaseAdd BLAST165

    Proteomic databases

    PaxDbiP76270.
    PRIDEiP76270.

    2D gel databases

    SWISS-2DPAGEP76270.

    Interactioni

    Protein-protein interaction databases

    BioGridi4259156. 10 interactors.
    DIPiDIP-11813N.
    IntActiP76270. 8 interactors.
    STRINGi511145.b1832.

    Structurei

    Secondary structure

    1165
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 18Combined sources16
    Helixi24 – 38Combined sources15
    Beta strandi39 – 41Combined sources3
    Beta strandi43 – 51Combined sources9
    Beta strandi54 – 63Combined sources10
    Beta strandi67 – 70Combined sources4
    Helixi74 – 82Combined sources9
    Beta strandi86 – 89Combined sources4
    Turni91 – 93Combined sources3
    Beta strandi105 – 114Combined sources10
    Beta strandi117 – 128Combined sources12
    Helixi134 – 152Combined sources19
    Helixi156 – 158Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1VHMX-ray2.10A/B1-165[»]
    ProteinModelPortaliP76270.
    SMRiP76270.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP76270.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini49 – 149GAFAdd BLAST101

    Sequence similaritiesi

    Contains 1 GAF domain.Curated

    Phylogenomic databases

    eggNOGiENOG4108ZGC. Bacteria.
    COG1956. LUCA.
    HOGENOMiHOG000022909.
    InParanoidiP76270.
    KOiK07170.
    OMAiFQGQPAC.
    PhylomeDBiP76270.

    Family and domain databases

    Gene3Di3.30.450.40. 1 hit.
    InterProiIPR000614. fRMsr_CS.
    IPR003018. GAF.
    IPR029016. GAF_dom-like.
    [Graphical view]
    PfamiPF13185. GAF_2. 1 hit.
    [Graphical view]
    SMARTiSM00065. GAF. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 1 hit.
    PROSITEiPS01320. UPF0067. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P76270-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNKTEFYADL NRDFNALMAG ETSFLATLAN TSALLYERLT DINWAGFYLL
    60 70 80 90 100
    EDDTLVLGPF QGKIACVRIP VGRGVCGTAV ARNQVQRIED VHVFDGHIAC
    110 120 130 140 150
    DAASNSEIVL PLVVKNQIIG VLDIDSTVFG RFTDEDEQGL RQLVAQLEKV
    160
    LATTDYKKFF ASVAG
    Length:165
    Mass (Da):18,122
    Last modified:December 4, 2007 - v2
    Checksum:iB49F1D1740DD5D43
    GO

    Sequence cautioni

    The sequence BAA15640 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC74902.2.
    AP009048 Genomic DNA. Translation: BAA15640.1. Different initiation.
    PIRiH64944.
    RefSeqiNP_416346.4. NC_000913.3.
    WP_001043877.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74902; AAC74902; b1832.
    BAA15640; BAA15640; BAA15640.
    GeneIDi946086.
    KEGGiecj:JW1821.
    eco:b1832.
    PATRICi32118985. VBIEscCol129921_1910.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC74902.2.
    AP009048 Genomic DNA. Translation: BAA15640.1. Different initiation.
    PIRiH64944.
    RefSeqiNP_416346.4. NC_000913.3.
    WP_001043877.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1VHMX-ray2.10A/B1-165[»]
    ProteinModelPortaliP76270.
    SMRiP76270.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259156. 10 interactors.
    DIPiDIP-11813N.
    IntActiP76270. 8 interactors.
    STRINGi511145.b1832.

    2D gel databases

    SWISS-2DPAGEP76270.

    Proteomic databases

    PaxDbiP76270.
    PRIDEiP76270.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC74902; AAC74902; b1832.
    BAA15640; BAA15640; BAA15640.
    GeneIDi946086.
    KEGGiecj:JW1821.
    eco:b1832.
    PATRICi32118985. VBIEscCol129921_1910.

    Organism-specific databases

    EchoBASEiEB3774.
    EcoGeneiEG14020. msrC.

    Phylogenomic databases

    eggNOGiENOG4108ZGC. Bacteria.
    COG1956. LUCA.
    HOGENOMiHOG000022909.
    InParanoidiP76270.
    KOiK07170.
    OMAiFQGQPAC.
    PhylomeDBiP76270.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7005-MONOMER.
    ECOL316407:JW1821-MONOMER.
    MetaCyc:G7005-MONOMER.
    BRENDAi1.8.4.14. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP76270.
    PROiP76270.

    Family and domain databases

    Gene3Di3.30.450.40. 1 hit.
    InterProiIPR000614. fRMsr_CS.
    IPR003018. GAF.
    IPR029016. GAF_dom-like.
    [Graphical view]
    PfamiPF13185. GAF_2. 1 hit.
    [Graphical view]
    SMARTiSM00065. GAF. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 1 hit.
    PROSITEiPS01320. UPF0067. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMSRC_ECOLI
    AccessioniPrimary (citable) accession number: P76270
    Secondary accession number(s): O07976, O07978
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 4, 2007
    Last modified: November 2, 2016
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.