P76264 (MNTP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable manganese efflux pump MntP

Gene names

Name:	mntP
Synonyms:	yebN
Ordered Locus Names:	b1821, JW5830

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably functions as a manganese efflux pump. Ref.6
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_01521.
Induction	Up-regulated by MntR in response to manganese. Ref.6
Disruption phenotype	Deletion causes manganese sensitivity and leads to increased intracellular manganese levels. Ref.6
Sequence similarities	Belongs to the MntP (TC 9.B.29) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Manganese
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to manganese ion Inferred from expression pattern Ref.6. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	manganese ion transmembrane transporter activity Inferred from mutant phenotype Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Probable manganese efflux pump MntP HAMAP-Rule MF_01521		PRO_0000155649
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Helical; Potential
	Topological domain	24 – 65	42	Periplasmic Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Topological domain	87 – 105	19	Cytoplasmic Potential
	Transmembrane	106 – 128	23	Helical; Potential
	Topological domain	129 – 142	14	Periplasmic Potential
	Transmembrane	143 – 163	21	Helical; Potential
	Topological domain	164 – 167	4	Cytoplasmic Potential
	Transmembrane	168 – 187	20	Helical; Potential
	Topological domain	188	1	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P76264 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 09BBB5C3D6185A9B	FASTA	188	20,117
	10 20 30 40 50 60 MNITATVLLA FGMSMDAFAA SIGKGATLHK PKFSEALRTG LIFGAVETLT PLIGWGMGML 70 80 90 100 110 120 ASRFVLEWNH WIAFVLLIFL GGRMIIEGFR GADDEDEEPR RRHGFWLLVT TAIATSLDAM 130 140 150 160 170 180 AVGVGLAFLQ VNIIATALAI GCATLIMSTL GMMVGRFIGS IIGKKAEILG GLVLIGIGVQ ILWTHFHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Fur regulon in Gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay." Stojiljkovic I., Baumler A.J., Hantke K. J. Mol. Biol. 236:531-545(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis." Waters L.S., Sandoval M., Storz G. J. Bacteriol. 193:5887-5897(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN MANGANESE HOMEOSTASIS, INDUCTION, DISRUPTION PHENOTYPE, GENE NAME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74891.2. AP009048 Genomic DNA. Translation: BAA15632.2. X75948 Genomic DNA. No translation available.
PIR	E64943.
RefSeq	NP_416335.4. NC_000913.2. YP_490082.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76264.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1821.
Protein family/group databases
TCDB	9.B.29.1.1. YebN family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74891; AAC74891; b1821. BAA15632; BAA15632; BAA15632.
GeneID	12934032. 946341.
KEGG	ecj:Y75_p1796. eco:b1821.
PATRIC	32118961. VBIEscCol129921_1898.
Organism-specific databases
EchoBASE	EB3768.
EcoGene	EG14012. mntP.
Phylogenomic databases
eggNOG	COG1971.
HOGENOM	HOG000238852.
OMA	AWDHWIA.
ProtClustDB	PRK11469.
Enzyme and pathway databases
BioCyc	EcoCyc:G6999-MONOMER. ECOL316407:JW5830-MONOMER.
Gene expression databases
Genevestigator	P76264.
Family and domain databases
HAMAP	MF_01521. MntP_pump.
InterPro	IPR003810. DUF204. IPR022929. UPF0059. [Graphical view]
Pfam	PF02659. DUF204. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNTP_ECOLI

Accession

Primary (citable) accession number: P76264
Secondary accession number(s): O07974

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program