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Protein

Probable ATP-dependent DNA helicase YoaA

Gene

yoaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase and 5'-3' DNA helicase (By similarity). Involved in the repair of replication forks and tolerance of the chain-terminating nucleoside analog 3' azidothymidine (AZT). May unwind potentially damaged 3' nascent ends such as those terminated by AZT, promote repair and AZT excision (PubMed:26544712).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi168Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi173Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi179Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • postreplication repair Source: EcoCyc

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6992-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent DNA helicase YoaACurated (EC:3.6.4.12By similarity)
Gene namesi
Name:yoaA
Ordered Locus Names:b1808, JW1797
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13513 yoaA

Pathology & Biotechi

Disruption phenotypei

Knockout mutants are sensitive to AZT.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51K → A: Cannot complement the AZT sensitivity of the knockout mutant. 1 Publication1
Mutagenesisi168C → A: Cannot complement the AZT sensitivity of the knockout mutant. 1 Publication1
Mutagenesisi225D → A: Cannot complement the AZT sensitivity of the knockout mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020061 – 636Probable ATP-dependent DNA helicase YoaAAdd BLAST636

Proteomic databases

PaxDbiP76257
PRIDEiP76257

Interactioni

Subunit structurei

Interacts with the DNA polymerase III subunit Chi (holC).1 Publication

Protein-protein interaction databases

BioGridi4260341, 111 interactors
DIPiDIP-12786N
IntActiP76257, 10 interactors
STRINGi316385.ECDH10B_1946

Structurei

3D structure databases

ProteinModelPortaliP76257
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 272Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST263

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 228DEAH boxPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the helicase family. DinG subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105DVT Bacteria
COG1199 LUCA
HOGENOMiHOG000242574
InParanoidiP76257
KOiK03722
OMAiAKIIVVN
PhylomeDBiP76257

Family and domain databases

InterProiView protein in InterPro
IPR006555 ATP-dep_Helicase_C
IPR014013 Helic_SF1/SF2_ATP-bd_DinG/Rad3
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF13307 Helicase_C_2, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00491 HELICc2, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
PROSITEiView protein in PROSITE
PS51193 HELICASE_ATP_BIND_2, 1 hit

Sequencei

Sequence statusi: Complete.

P76257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDFAPDGQ LAKAIPGFKP REPQRQMAVA VTQAIEKGQP LVVEAGTGTG
60 70 80 90 100
KTYAYLAPAL RAKKKVIIST GSKALQDQLY SRDLPTVSKA LKYTGNVALL
110 120 130 140 150
KGRSNYLCLE RLEQQALAGG DLPVQILSDV ILLRSWSNQT VDGDISTCVS
160 170 180 190 200
VAEDSQAWPL VTSTNDNCLG SDCPMYKDCF VVKARKKAMD ADVVVVNHHL
210 220 230 240 250
FLADMVVKES GFGELIPEAD VMIFDEAHQL PDIASQYFGQ SLSSRQLLDL
260 270 280 290 300
AKDITIAYRT ELKDTQQLQK CADRLAQSAQ DFRLQLGEPG YRGNLRELLA
310 320 330 340 350
NPQIQRAFLL LDDTLELCYD VAKLSLGRSA LLDAAFERAT LYRTRLKRLK
360 370 380 390 400
EINQPGYSYW YECTSRHFTL ALTPLSVADK FKELMAQKPG SWIFTSATLS
410 420 430 440 450
VNDDLHHFTS RLGIEQAESL LLPSPFDYSR QALLCVLRNL PQTNQPGSAR
460 470 480 490 500
QLAAMLRPII EANNGRCFML CTSHAMMRDL AEQFRATMTL PVLLQGETSK
510 520 530 540 550
GQLLQQFVSA GNALLVATSS FWEGVDVRGD TLSLVIIDKL PFTSPDDPLL
560 570 580 590 600
KARMEDCRLR GGDPFDEVQL PDAVITLKQG VGRLIRDADD RGVLVICDNR
610 620 630
LVMRPYGATF LASLPPAPRT RDIARAVRFL AIPSSR
Length:636
Mass (Da):70,378
Last modified:February 1, 1997 - v1
Checksum:i14CEBD04CCBE701F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74878.1
AP009048 Genomic DNA Translation: BAA15617.1
PIRiH64941
RefSeqiNP_416322.1, NC_000913.3
WP_000128841.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74878; AAC74878; b1808
BAA15617; BAA15617; BAA15617
GeneIDi946305
KEGGiecj:JW1797
eco:b1808
PATRICifig|511145.12.peg.1884

Similar proteinsi

Entry informationi

Entry nameiYOAA_ECOLI
AccessioniPrimary (citable) accession number: P76257
Secondary accession number(s): O07973
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 25, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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