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Protein

Carnitine monooxygenase reductase subunit

Gene

yeaX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts carnitine to trimethylamine and malic semialdehyde. Can also use gamma-butyrobetaine, choline and betaine as substrates.1 Publication

Catalytic activityi

L-carnitine + NAD(P)H + H+ + O2 = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)+ + H2O.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FMNUniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 2Fe-2S cluster.UniRule annotation

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi270Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi275Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi278Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi308Iron-sulfur (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6989-MONOMER
MetaCyc:G6989-MONOMER
UniPathwayiUPA00117

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine monooxygenase reductase subunitUniRule annotationCurated (EC:1.14.13.-UniRule annotation1 Publication)
Alternative name(s):
Carnitine monooxygenase beta subunitUniRule annotationCurated
Gene namesi
Name:yeaX
Ordered Locus Names:b1803, JW1792
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13510 yeaX

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001894051 – 321Carnitine monooxygenase reductase subunitAdd BLAST321

Proteomic databases

PaxDbiP76254
PRIDEiP76254

Interactioni

Subunit structurei

Composed of an oxygenase subunit (yeaW) and a reductase subunit (yeaX).By similarity

Protein-protein interaction databases

BioGridi4260337, 16 interactors
STRINGi316385.ECDH10B_1941

Structurei

3D structure databases

ProteinModelPortaliP76254
SMRiP76254
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 109FAD-binding FR-typeUniRule annotationAdd BLAST106
Domaini233 – 3212Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the PDR/VanB family. CntB subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105E3J Bacteria
COG1018 LUCA
HOGENOMiHOG000141052
InParanoidiP76254
KOiK22444
OMAiVICCCVS
PhylomeDBiP76254

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
HAMAPiMF_02098 Carnitine_monoox_B, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR039003 Carnitine_monoox_B
IPR017927 Fd_Rdtase_FAD-bd
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000951 Ph_dOase_redase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00409 PHDIOXRDTASE
SUPFAMiSSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

P76254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYQMFEVQ VSQVEPLTEQ VKRFTLVATD GKPLPAFTGG SHVIVQMSDG
60 70 80 90 100
DNQYSNAYSL LSSPHDTSCY QIAVRLEENS RGGSRFLHQQ VKVGDRLTIS
110 120 130 140 150
TPNNLFALIP SARKHLFIAG GIGITPFLSH MAELQHSDVD WQLHYCSRNP
160 170 180 190 200
ESCAFRDELV QHPQAEKVHL HHSSTGTRLE LARLLADIEP GTHVYTCGPE
210 220 230 240 250
ALIEAVRSEA ARLDIAADTL HFEQFAIEDK TGDAFTLVLA RSGKEFVVPE
260 270 280 290 300
EMTILQVIEN NKAAKVECLC REGVCGTCET AILEGEADHR DQYFSDEERA
310 320
SQQSMLICCS RAKGKRLVLD L
Length:321
Mass (Da):35,661
Last modified:February 1, 1997 - v1
Checksum:iBD34122B24C08DBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74873.1
AP009048 Genomic DNA Translation: BAA15598.1
PIRiC64941
RefSeqiNP_416317.1, NC_000913.3
WP_001287026.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74873; AAC74873; b1803
BAA15598; BAA15598; BAA15598
GeneIDi946329
KEGGiecj:JW1792
eco:b1803
PATRICifig|1411691.4.peg.450

Similar proteinsi

Entry informationi

Entry nameiCNTB_ECOLI
AccessioniPrimary (citable) accession number: P76254
Secondary accession number(s): O07970, O07972
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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