Reviewed, UniProtKB/Swiss-Prot P76251 (TTUC_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable tartrate dehydrogenase/decarboxylase
      Short name=TDH
    EC=1.1.1.93
    EC=4.1.1.73
Alternative name(s):
    D-malate dehydrogenase [decarboxylating]
    EC=1.1.1.83

Gene names

Name:	yeaU
Ordered Locus Names:	b1800, JW1789

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Tartrate + NAD(+) = oxaloglycolate + NADH. Meso-tartrate + NAD(+) = oxaloglycolate + NADH. (R,R)-tartrate + NAD(+) = oxaloglycolate + NADH. (R,R)-tartrate = D-glycerate + CO(2). (R)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Lyase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-malate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct tartrate decarboxylase activity Inferred from electronic annotation. Source: EC tartrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-560661,EBI-543750
tufA	P0A6N1	1	EBI-560661,EBI-301077

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Probable tartrate dehydrogenase/decarboxylase		PRO_0000083818

Sequences

	Sequence		Length	Mass (Da)
	P76251-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6D77B609729925D7	FASTA	361	40,315
	10 20 30 40 50 60 MMKTMRIAAI PGDGIGKEVL PEGIRVLQAA AERWGFALSF EQMEWASCEY YSHHGKMMPD 70 80 90 100 110 120 DWHEQLSRFD AIYFGAVGWP DTVPDHISLW GSLLKFRREF DQYVNLRPVR LFPGVPCPLA 130 140 150 160 170 180 GKQPGDIDFY VVRENTEGEY SSLGGRVNEG TEHEVVIQES VFTRRGVDRI LRYAFELAQS 190 200 210 220 230 240 RPRKTLTSAT KSNGLAISMP YWDERVEAMA ENYPEIRWDK QHIDILCARF VMQPERFDVV 250 260 270 280 290 300 VASNLFGDIL SDLGPACTGT IGIAPSANLN PERTFPSLFE PVHGSAPDIY GKNIANPIAT 310 320 330 340 350 360 IWAGAMMLDF LGNGDERFQQ AHNGILAAIE EVIAHGPKTP DMKGNATTPQ VADAICKIIL R

References

[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC74870.1. AP009048 Genomic DNA. Translation: BAA15595.1.
PIR	H64940.
RefSeq	AP_002419.1. NP_416314.1.
3D structure databases
HSSP	HSSP built from PDB template 1CM7 based on UniProtKB P30125.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:11800N.
IntAct	P76251.
Genome annotation databases
GeneID	946319.
GenomeReviews	Gene locus b1800 in contig U00096_GR. Gene locus JW1789 in contig AP009048_GR.
KEGG	ecj:JW1789. eco:b1800.
Organism-specific databases
EchoBASE	EB3280.
EcoGene	EG13507. yeaU.
CMR	Search...
Phylogenomic databases
HOGENOM	P76251.
Enzyme and pathway databases
BioCyc	EcoCyc:G6986-MON. MetaCyc:G6986-MON.
Family and domain databases
InterPro	IPR001804. IsoCit_IM_DHase. IPR011829. TTC_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF8. TTC_DH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR02089. TTC. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P76251.

Entry information

Entry name

TTUC_ECOLI

Accession

Primary (citable) accession number: P76251
Secondary accession number(s): O08474, O08475

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)