Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine efflux protein

Gene

leuE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exporter of leucine. Can also transport its natural analog L-alpha-amino-n-butyric acid and some other structurally unrelated amino acids.1 Publication

GO - Molecular functioni

  • L-leucine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • L-alpha-amino acid transmembrane transport Source: GOC
  • L-amino acid transport Source: GOC
  • leucine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6984-MONOMER.
ECOL316407:JW1787-MONOMER.
MetaCyc:G6984-MONOMER.

Protein family/group databases

TCDBi2.A.76.1.5. the resistance to homoserine/threonine (rhtb) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine efflux protein
Gene namesi
Name:leuE
Synonyms:yeaS
Ordered Locus Names:b1798, JW1787
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13505. leuE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence analysisAdd
BLAST
Transmembranei122 – 14221HelicalSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Leucine efflux proteinPRO_0000094742Add
BLAST

Proteomic databases

PaxDbiP76249.
PRIDEiP76249.

Expressioni

Inductioni

Induced by leucine, L-alpha-amino-n-butyric acid and, to a lesser extent, by several other amino acids. This induction is mediated by lrp.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260333. 22 interactions.
STRINGi511145.b1798.

Structurei

3D structure databases

ProteinModelPortaliP76249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rht family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108NIW. Bacteria.
COG1280. LUCA.
HOGENOMiHOG000122945.
InParanoidiP76249.
KOiK11250.
OMAiFGVLNYW.
OrthoDBiEOG66XBFF.
PhylomeDBiP76249.

Family and domain databases

InterProiIPR001123. LysE-type.
[Graphical view]
PANTHERiPTHR30086. PTHR30086. 1 hit.
PfamiPF01810. LysE. 1 hit.
[Graphical view]
PIRSFiPIRSF006324. LeuE. 1 hit.

Sequencei

Sequence statusi: Complete.

P76249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAEYGVLNY WTYLVGAIFI VLVPGPNTLF VLKNSVSSGM KGGYLAACGV
60 70 80 90 100
FIGDAVLMFL AWAGVATLIK TTPILFNIVR YLGAFYLLYL GSKILYATLK
110 120 130 140 150
GKNSEAKSDE PQYGAIFKRA LILSLTNPKA ILFYVSFFVQ FIDVNAPHTG
160 170 180 190 200
ISFFILAATL ELVSFCYLSF LIISGAFVTQ YIRTKKKLAK VGNSLIGLMF
210
VGFAARLATL QS
Length:212
Mass (Da):23,201
Last modified:February 1, 1997 - v1
Checksum:i342E0DF348C9AD9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74868.1.
AP009048 Genomic DNA. Translation: BAA15593.1.
PIRiF64940.
RefSeqiNP_416312.1. NC_000913.3.
WP_000457206.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74868; AAC74868; b1798.
BAA15593; BAA15593; BAA15593.
GeneIDi946157.
KEGGiecj:JW1787.
eco:b1798.
PATRICi32118913. VBIEscCol129921_1874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74868.1.
AP009048 Genomic DNA. Translation: BAA15593.1.
PIRiF64940.
RefSeqiNP_416312.1. NC_000913.3.
WP_000457206.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260333. 22 interactions.
STRINGi511145.b1798.

Protein family/group databases

TCDBi2.A.76.1.5. the resistance to homoserine/threonine (rhtb) family.

Proteomic databases

PaxDbiP76249.
PRIDEiP76249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74868; AAC74868; b1798.
BAA15593; BAA15593; BAA15593.
GeneIDi946157.
KEGGiecj:JW1787.
eco:b1798.
PATRICi32118913. VBIEscCol129921_1874.

Organism-specific databases

EchoBASEiEB3278.
EcoGeneiEG13505. leuE.

Phylogenomic databases

eggNOGiENOG4108NIW. Bacteria.
COG1280. LUCA.
HOGENOMiHOG000122945.
InParanoidiP76249.
KOiK11250.
OMAiFGVLNYW.
OrthoDBiEOG66XBFF.
PhylomeDBiP76249.

Enzyme and pathway databases

BioCyciEcoCyc:G6984-MONOMER.
ECOL316407:JW1787-MONOMER.
MetaCyc:G6984-MONOMER.

Miscellaneous databases

PROiP76249.

Family and domain databases

InterProiIPR001123. LysE-type.
[Graphical view]
PANTHERiPTHR30086. PTHR30086. 1 hit.
PfamiPF01810. LysE. 1 hit.
[Graphical view]
PIRSFiPIRSF006324. LeuE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The yeaS (leuE) gene of Escherichia coli encodes an exporter of leucine, and the Lrp protein regulates its expression."
    Kutukova E.A., Livshits V.A., Altman I.P., Ptitsyn L.R., Zyiatdinov M.H., Tokmakova I.L., Zakataeva N.P.
    FEBS Lett. 579:4629-4634(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LEUCINE EFFLUX, INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiLEUE_ECOLI
AccessioniPrimary (citable) accession number: P76249
Secondary accession number(s): O07969, O07971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.