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Protein

Probable diguanylate cyclase YeaP

Gene

yeaP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger (Probable). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.Curated1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi212 – 2121MagnesiumBy similarity
Sitei217 – 2171Transition state stabilizerSequence analysis
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei229 – 2291SubstrateBy similarity
Active sitei255 – 2551Proton acceptorSequence analysis
Metal bindingi255 – 2551MagnesiumBy similarity

GO - Molecular functioni

  • diguanylate cyclase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6980-MONOMER.
ECOL316407:JW5292-MONOMER.
MetaCyc:G6980-MONOMER.
UniPathwayiUPA00599.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase YeaP (EC:2.7.7.65)
Short name:
DGC
Gene namesi
Name:yeaP
Ordered Locus Names:b1794, JW5292
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13502. yeaP.

Pathology & Biotechi

Disruption phenotypei

Decreased expression of CsgB, decreased curli expression at 28 degrees Celsius.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Probable diguanylate cyclase YeaPPRO_0000169024Add
BLAST

Proteomic databases

PaxDbiP76245.
PRIDEiP76245.

Expressioni

Inductioni

Expressed during exponential and post-exponential growth at both 28 and 37 degrees Celsius.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4260330. 17 interactions.
DIPiDIP-11795N.
IntActiP76245. 3 interactions.
MINTiMINT-1321161.
STRINGi511145.b1794.

Structurei

3D structure databases

ProteinModelPortaliP76245.
SMRiP76245. Positions 92-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 154137GAFAdd
BLAST
Domaini204 – 337134GGDEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 GGDEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410620I. Bacteria.
COG2203. LUCA.
HOGENOMiHOG000279862.
InParanoidiP76245.
KOiK13069.
OMAiDTLCKRA.
OrthoDBiEOG61P6NF.
PhylomeDBiP76245.

Family and domain databases

Gene3Di3.30.450.40. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF00990. GGDEF. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 1 hit.
SM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQIIARVS QSLAKEQSLE SLVRQLLEML EMVTDMESTY LTKVDVEARL
60 70 80 90 100
QHIMFARNSQ KMYIPENFTV SWDYSLCKRA IDENCFFSDE VPDRWGDCIA
110 120 130 140 150
ARNLGITTFL STPIHLPDGS FYGTLCAASS EKRQWSERAE QVLQLFAGLI
160 170 180 190 200
AQYIQKEALV EQLREANAAL IAQSYTDSLT GLPNRRAIFE NLTTLFSLAR
210 220 230 240 250
HLNHKIMIAF IDLDNFKLIN DRFGHNSGDL FLIQVGERLN TLQQNGEVIG
260 270 280 290 300
RLGGDEFLVV SLNNENADIS SLRERIQQQI RGEYHLGDVD LYYPGASLGI
310 320 330 340
VEVDPETTDA DSALHAADIA MYQEKKHKQK TPFVAHPALH S
Length:341
Mass (Da):38,546
Last modified:April 27, 2001 - v2
Checksum:i6A8EA4053E59A4E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74864.2.
AP009048 Genomic DNA. Translation: BAA15592.1.
PIRiB64940.
RefSeqiNP_416308.4. NC_000913.3.
WP_001310896.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74864; AAC74864; b1794.
BAA15592; BAA15592; BAA15592.
GeneIDi948969.
KEGGiecj:JW5292.
eco:b1794.
PATRICi32118901. VBIEscCol129921_1868.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74864.2.
AP009048 Genomic DNA. Translation: BAA15592.1.
PIRiB64940.
RefSeqiNP_416308.4. NC_000913.3.
WP_001310896.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76245.
SMRiP76245. Positions 92-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260330. 17 interactions.
DIPiDIP-11795N.
IntActiP76245. 3 interactions.
MINTiMINT-1321161.
STRINGi511145.b1794.

Proteomic databases

PaxDbiP76245.
PRIDEiP76245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74864; AAC74864; b1794.
BAA15592; BAA15592; BAA15592.
GeneIDi948969.
KEGGiecj:JW5292.
eco:b1794.
PATRICi32118901. VBIEscCol129921_1868.

Organism-specific databases

EchoBASEiEB3275.
EcoGeneiEG13502. yeaP.

Phylogenomic databases

eggNOGiENOG410620I. Bacteria.
COG2203. LUCA.
HOGENOMiHOG000279862.
InParanoidiP76245.
KOiK13069.
OMAiDTLCKRA.
OrthoDBiEOG61P6NF.
PhylomeDBiP76245.

Enzyme and pathway databases

UniPathwayiUPA00599.
BioCyciEcoCyc:G6980-MONOMER.
ECOL316407:JW5292-MONOMER.
MetaCyc:G6980-MONOMER.

Miscellaneous databases

PROiP76245.

Family and domain databases

Gene3Di3.30.450.40. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF00990. GGDEF. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 1 hit.
SM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Cyclic diguanylate is a ubiquitous signaling molecule in bacteria: insights into biochemistry of the GGDEF protein domain."
    Ryjenkov D.A., Tarutina M., Moskvin O.V., Gomelsky M.
    J. Bacteriol. 187:1792-1798(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DIGUANYLATE CYCLASE.
    Strain: K12 / DH5-alpha.
  5. "Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli."
    Sommerfeldt N., Possling A., Becker G., Pesavento C., Tschowri N., Hengge R.
    Microbiology 155:1318-1331(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiYEAP_ECOLI
AccessioniPrimary (citable) accession number: P76245
Secondary accession number(s): P94742, P97193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 27, 2001
Last modified: January 20, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.