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Protein

Diguanylate cyclase DgcP

Gene

dgcP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi212MagnesiumBy similarity1
Sitei217Transition state stabilizerSequence analysis1
Binding sitei220SubstrateBy similarity1
Binding sitei225Substrate; via carbonyl oxygenBy similarity1
Binding sitei229SubstrateBy similarity1
Active sitei255Proton acceptorSequence analysis1
Metal bindingi255MagnesiumBy similarity1

GO - Molecular functioni

  • diguanylate cyclase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6980-MONOMER
MetaCyc:G6980-MONOMER
UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Diguanylate cyclase DgcPCurated (EC:2.7.7.651 Publication)
Short name:
DGCCurated
Gene namesi
Name:dgcP1 Publication
Synonyms:yeaP
Ordered Locus Names:b1794, JW5292
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13502 yeaP

Pathology & Biotechi

Disruption phenotypei

Decreased expression of CsgB, decreased curli expression at 28 degrees Celsius.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690241 – 341Diguanylate cyclase DgcPAdd BLAST341

Proteomic databases

PaxDbiP76245
PRIDEiP76245

Expressioni

Inductioni

Expressed during exponential and post-exponential growth at both 28 and 37 degrees Celsius.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4260330, 23 interactors
DIPiDIP-11795N
IntActiP76245, 3 interactors
STRINGi316385.ECDH10B_1932

Structurei

3D structure databases

ProteinModelPortaliP76245
SMRiP76245
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 154GAFAdd BLAST137
Domaini204 – 337GGDEFPROSITE-ProRule annotationAdd BLAST134

Phylogenomic databases

eggNOGiENOG410620I Bacteria
COG2203 LUCA
HOGENOMiHOG000279862
InParanoidiP76245
KOiK13069
OMAiDTLCKRA
PhylomeDBiP76245

Family and domain databases

CDDicd01949 GGDEF, 1 hit
Gene3Di3.30.450.40, 1 hit
InterProiView protein in InterPro
IPR003018 GAF
IPR029016 GAF-like_dom_sf
IPR000160 GGDEF_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF13185 GAF_2, 1 hit
PF00990 GGDEF, 1 hit
SMARTiView protein in SMART
SM00065 GAF, 1 hit
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

P76245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQIIARVS QSLAKEQSLE SLVRQLLEML EMVTDMESTY LTKVDVEARL
60 70 80 90 100
QHIMFARNSQ KMYIPENFTV SWDYSLCKRA IDENCFFSDE VPDRWGDCIA
110 120 130 140 150
ARNLGITTFL STPIHLPDGS FYGTLCAASS EKRQWSERAE QVLQLFAGLI
160 170 180 190 200
AQYIQKEALV EQLREANAAL IAQSYTDSLT GLPNRRAIFE NLTTLFSLAR
210 220 230 240 250
HLNHKIMIAF IDLDNFKLIN DRFGHNSGDL FLIQVGERLN TLQQNGEVIG
260 270 280 290 300
RLGGDEFLVV SLNNENADIS SLRERIQQQI RGEYHLGDVD LYYPGASLGI
310 320 330 340
VEVDPETTDA DSALHAADIA MYQEKKHKQK TPFVAHPALH S
Length:341
Mass (Da):38,546
Last modified:April 27, 2001 - v2
Checksum:i6A8EA4053E59A4E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74864.2
AP009048 Genomic DNA Translation: BAA15592.1
PIRiB64940
RefSeqiNP_416308.4, NC_000913.3
WP_001310896.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74864; AAC74864; b1794
BAA15592; BAA15592; BAA15592
GeneIDi948969
KEGGiecj:JW5292
eco:b1794
PATRICifig|1411691.4.peg.461

Similar proteinsi

Entry informationi

Entry nameiDGCP_ECOLI
AccessioniPrimary (citable) accession number: P76245
Secondary accession number(s): P94742, P97193
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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