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Protein

Probable diguanylate cyclase DgcJ

Gene

dgcJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules.By similarity

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi382MagnesiumBy similarity1
Sitei387Transition state stabilizerSequence analysis1
Binding sitei390SubstrateBy similarity1
Binding sitei395Substrate; via carbonyl oxygenBy similarity1
Binding sitei399SubstrateBy similarity1
Active sitei425Proton acceptorSequence analysis1
Metal bindingi425MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6972-MONOMER
UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase DgcJCurated (EC:2.7.7.65By similarity)
Short name:
DGCCurated
Gene namesi
Name:dgcJ1 Publication
Synonyms:yeaJ
Ordered Locus Names:b1786, JW5291
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13496 yeaJ

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei305 – 325HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene increases swimming motility (PubMed:21181144). Disruption of this gene partially suppresses the reduced motility of a pdeH disruption. Full suppression i.e. wild-type motility, requires concomitant disruption of dgcJ, dgcE and dgcQ (PubMed:18765794).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690161 – 496Probable diguanylate cyclase DgcJAdd BLAST496

Proteomic databases

PaxDbiP76237
PRIDEiP76237

Expressioni

Inductioni

Expressed during exponential and post-exponential growth, it is slightly more highly expressed at 37 than 28 degrees Celsius and is more highly induced on solid medium.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
mdtOP327152EBI-555763,EBI-555775

Protein-protein interaction databases

BioGridi4260325, 7 interactors
DIPiDIP-11789N
IntActiP76237, 2 interactors
STRINGi316385.ECDH10B_1924

Structurei

3D structure databases

ProteinModelPortaliP76237
SMRiP76237
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 496GGDEFPROSITE-ProRule annotationAdd BLAST123

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108KHP Bacteria
COG2199 LUCA
HOGENOMiHOG000121142
InParanoidiP76237
OMAiSINRAQD
PhylomeDBiP76237

Family and domain databases

CDDicd01949 GGDEF, 1 hit
InterProiView protein in InterPro
IPR033420 GAPES1
IPR000160 GGDEF_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF17155 GAPES1, 1 hit
PF00990 GGDEF, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

P76237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLHHRMLRH FIAASVIVLT SSFLIFELVA SDRAMSAYLR YIVQKADSSF
60 70 80 90 100
LYDKYQNQSI AAHVMRALAA EQSEVSPEQR RAICEAFESA NNTHGLNLTA
110 120 130 140 150
HKYPGLRGTL QTASTDCDTI VEAAALLPAF DQAVEGNRHQ DDYGSGLGMA
160 170 180 190 200
EEKFHYYLDL NDRYVYFYEP VNVEYFAMNN WSFLQSGSIG IDRKDIEKVF
210 220 230 240 250
TGRTVLSSIY QDQRTKQNVM SLLTPVYVAG QLKGIVLLDI NKNNLRNIFY
260 270 280 290 300
THDRPLLWRF LNVTLTDTDS GRDIIINQSE DNLFQYVSYV HDLPGGIRVS
310 320 330 340 350
LSIDILYFIT SSWKSVLFWI LTALILLNMV RMHFRLYQNV SRENISDAMT
360 370 380 390 400
GLYNRKILTP ELEQRLQKLV QSGSSVMFIA IDMDKLKQIN DTLGHQEGDL
410 420 430 440 450
AITLLAQAIK QSIRKSDYAI RLGGDEFCII LVDSTPQIAA QLPERIEKRL
460 470 480 490
QHIAPQKEIG FSSGIYAMKE NDTLHDAYKA SDERLYVNKQ NKNSRS
Length:496
Mass (Da):56,603
Last modified:December 1, 2000 - v2
Checksum:i8E66D32CDB6F05DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74856.2
AP009048 Genomic DNA Translation: BAA15587.2
PIRiB64939
RefSeqiNP_416300.2, NC_000913.3
WP_000766132.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74856; AAC74856; b1786
BAA15587; BAA15587; BAA15587
GeneIDi946290
KEGGiecj:JW5291
eco:b1786
PATRICifig|1411691.4.peg.468

Similar proteinsi

Entry informationi

Entry nameiDGCJ_ECOLI
AccessioniPrimary (citable) accession number: P76237
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: April 25, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health