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Protein

Putative diguanylate cyclase YeaJ

Gene

yeaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi382 – 3821MagnesiumBy similarity
Sitei387 – 3871Transition state stabilizerSequence analysis
Binding sitei390 – 3901SubstrateBy similarity
Binding sitei399 – 3991SubstrateBy similarity
Active sitei425 – 4251Proton acceptorSequence analysis
Metal bindingi425 – 4251MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6972-MONOMER.
ECOL316407:JW5291-MONOMER.
UniPathwayiUPA00599.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative diguanylate cyclase YeaJ (EC:2.7.7.65)
Short name:
DGC
Gene namesi
Name:yeaJ
Ordered Locus Names:b1786, JW5291
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13496. yeaJ.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Transmembranei305 – 32521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Disruption of this gene partially suppresses the reduced motility of a yhjH disruption. Full suppression i.e. wild-type motility, requires concomitant disruption of yeaJ, yegE and yedQ.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Putative diguanylate cyclase YeaJPRO_0000169016Add
BLAST

Proteomic databases

PaxDbiP76237.
PRIDEiP76237.

Expressioni

Inductioni

Expressed during exponential and post-exponential growth, it is slightly more highly expressed at 37 than 28 degrees Celsius and is more highly induced on solid medium.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
mdtOP327152EBI-555763,EBI-555775

Protein-protein interaction databases

BioGridi4260325. 7 interactions.
DIPiDIP-11789N.
IntActiP76237. 2 interactions.
MINTiMINT-1239476.
STRINGi511145.b1786.

Structurei

3D structure databases

ProteinModelPortaliP76237.
SMRiP76237. Positions 305-495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini374 – 496123GGDEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GGDEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108KHP. Bacteria.
COG2199. LUCA.
HOGENOMiHOG000121142.
InParanoidiP76237.
OMAiSINRAQD.
OrthoDBiEOG6CP3RM.
PhylomeDBiP76237.

Family and domain databases

InterProiIPR033420. GAPES1.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF17155. GAPES1. 1 hit.
PF00990. GGDEF. 1 hit.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLHHRMLRH FIAASVIVLT SSFLIFELVA SDRAMSAYLR YIVQKADSSF
60 70 80 90 100
LYDKYQNQSI AAHVMRALAA EQSEVSPEQR RAICEAFESA NNTHGLNLTA
110 120 130 140 150
HKYPGLRGTL QTASTDCDTI VEAAALLPAF DQAVEGNRHQ DDYGSGLGMA
160 170 180 190 200
EEKFHYYLDL NDRYVYFYEP VNVEYFAMNN WSFLQSGSIG IDRKDIEKVF
210 220 230 240 250
TGRTVLSSIY QDQRTKQNVM SLLTPVYVAG QLKGIVLLDI NKNNLRNIFY
260 270 280 290 300
THDRPLLWRF LNVTLTDTDS GRDIIINQSE DNLFQYVSYV HDLPGGIRVS
310 320 330 340 350
LSIDILYFIT SSWKSVLFWI LTALILLNMV RMHFRLYQNV SRENISDAMT
360 370 380 390 400
GLYNRKILTP ELEQRLQKLV QSGSSVMFIA IDMDKLKQIN DTLGHQEGDL
410 420 430 440 450
AITLLAQAIK QSIRKSDYAI RLGGDEFCII LVDSTPQIAA QLPERIEKRL
460 470 480 490
QHIAPQKEIG FSSGIYAMKE NDTLHDAYKA SDERLYVNKQ NKNSRS
Length:496
Mass (Da):56,603
Last modified:December 1, 2000 - v2
Checksum:i8E66D32CDB6F05DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74856.2.
AP009048 Genomic DNA. Translation: BAA15587.2.
PIRiB64939.
RefSeqiNP_416300.2. NC_000913.3.
WP_000766132.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74856; AAC74856; b1786.
BAA15587; BAA15587; BAA15587.
GeneIDi946290.
KEGGiecj:JW5291.
eco:b1786.
PATRICi32118883. VBIEscCol129921_1859.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74856.2.
AP009048 Genomic DNA. Translation: BAA15587.2.
PIRiB64939.
RefSeqiNP_416300.2. NC_000913.3.
WP_000766132.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76237.
SMRiP76237. Positions 305-495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260325. 7 interactions.
DIPiDIP-11789N.
IntActiP76237. 2 interactions.
MINTiMINT-1239476.
STRINGi511145.b1786.

Proteomic databases

PaxDbiP76237.
PRIDEiP76237.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74856; AAC74856; b1786.
BAA15587; BAA15587; BAA15587.
GeneIDi946290.
KEGGiecj:JW5291.
eco:b1786.
PATRICi32118883. VBIEscCol129921_1859.

Organism-specific databases

EchoBASEiEB3269.
EcoGeneiEG13496. yeaJ.

Phylogenomic databases

eggNOGiENOG4108KHP. Bacteria.
COG2199. LUCA.
HOGENOMiHOG000121142.
InParanoidiP76237.
OMAiSINRAQD.
OrthoDBiEOG6CP3RM.
PhylomeDBiP76237.

Enzyme and pathway databases

UniPathwayiUPA00599.
BioCyciEcoCyc:G6972-MONOMER.
ECOL316407:JW5291-MONOMER.

Miscellaneous databases

PROiP76237.

Family and domain databases

InterProiIPR033420. GAPES1.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF17155. GAPES1. 1 hit.
PF00990. GGDEF. 1 hit.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli."
    Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R.
    Genes Dev. 22:2434-2446(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli."
    Sommerfeldt N., Possling A., Becker G., Pesavento C., Tschowri N., Hengge R.
    Microbiology 155:1318-1331(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiYEAJ_ECOLI
AccessioniPrimary (citable) accession number: P76237
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.