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Protein

N-succinylglutamate 5-semialdehyde dehydrogenase

Gene

astD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate. Also shows activity with decanal or succinic semialdehyde as the electron donor and NAD as the electron acceptor. No activity is detected with NADP as the electron acceptor. Therefore, is an aldehyde dehydrogenase with broad substrate specificity.1 Publication

Catalytic activityi

N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH.

Kineticsi

  1. KM=91 µM for decanal1 Publication
  2. KM=160 µM for NAD (at pH 8.5)1 Publication
  3. KM=1.72 mM for succinic semialdehyde (at pH 8.5)1 Publication
  1. Vmax=5.0 µmol/min/mg enzyme with decanal as substrate1 Publication
  2. Vmax=0.69 µmol/min/mg enzyme with succinic semialdehyde as substrate1 Publication

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 4 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.1 Publication
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase (astA)
  2. N-succinylarginine dihydrolase (astB)
  3. Succinylornithine transaminase (astC)
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei243 – 2431By similarity
Active sitei277 – 2771By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2256NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Arginine metabolism, Stress response

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:SUCCGLUALDDEHYD-MONOMER.
ECOL316407:JW5282-MONOMER.
MetaCyc:SUCCGLUALDDEHYD-MONOMER.
BRENDAi1.2.1.3. 2026.
UniPathwayiUPA00185; UER00282.

Names & Taxonomyi

Protein namesi
Recommended name:
N-succinylglutamate 5-semialdehyde dehydrogenase (EC:1.2.1.71)
Alternative name(s):
Succinylglutamic semialdehyde dehydrogenase
Short name:
SGSD
Gene namesi
Name:astD
Synonyms:ydjU
Ordered Locus Names:b1746, JW5282
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13997. astD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492N-succinylglutamate 5-semialdehyde dehydrogenasePRO_0000056569Add
BLAST

Proteomic databases

EPDiP76217.
PaxDbiP76217.

Expressioni

Inductioni

By nitrogen starvation, and arginine. Induced at stationary phase by sigma S.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262236. 13 interactions.
DIPiDIP-9186N.
IntActiP76217. 1 interaction.
STRINGi511145.b1746.

Structurei

3D structure databases

ProteinModelPortaliP76217.
SMRiP76217. Positions 2-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271506.
InParanoidiP76217.
KOiK06447.
OMAiMDAAVHL.
OrthoDBiEOG6BS8QW.
PhylomeDBiP76217.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_01174. Aldedh_AstD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR017649. SuccinylGlu_semiald_DH_AstD.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR03240. arg_catab_astD. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLWINGDWI TGQGASRVKR NPVSGEVLWQ GNDADAAQVE QACRAARAAF
60 70 80 90 100
PRWARLSFAE RHAVVERFAA LLESNKAELT AIIARETGKP RWEAATEVTA
110 120 130 140 150
MINKIAISIK AYHVRTGEQR SEMPDGAASL RHRPHGVLAV FGPYNFPGHL
160 170 180 190 200
PNGHIVPALL AGNTIIFKPS ELTPWSGEAV MRLWQQAGLP PGVLNLVQGG
210 220 230 240 250
RETGQALSAL EDLDGLLFTG SANTGYQLHR QLSGQPEKIL ALEMGGNNPL
260 270 280 290 300
IIDEVADIDA AVHLTIQSAF VTAGQRCTCA RRLLLKSGAQ GDAFLARLVA
310 320 330 340 350
VSQRLTPGNW DDEPQPFIGG LISEQAAQQV VTAWQQLEAM GGRPLLAPRL
360 370 380 390 400
LQAGTSLLTP GIIEMTGVAG VPDEEVFGPL LRVWRYDTFD EAIRMANNTR
410 420 430 440 450
FGLSCGLVSP EREKFDQLLL EARAGIVNWN KPLTGAASTA PFGGIGASGN
460 470 480 490
HRPSAWYAAD YCAWPMASLE SDSLTLPATL NPGLDFSDEV VR
Length:492
Mass (Da):53,026
Last modified:February 1, 1997 - v1
Checksum:iCA01ACE48D9CA8DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74816.1.
AP009048 Genomic DNA. Translation: BAA15538.2.
PIRiB64934.
RefSeqiNP_416260.1. NC_000913.3.
WP_000177206.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74816; AAC74816; b1746.
BAA15538; BAA15538; BAA15538.
GeneIDi946260.
KEGGiecj:JW5282.
eco:b1746.
PATRICi32118801. VBIEscCol129921_1818.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74816.1.
AP009048 Genomic DNA. Translation: BAA15538.2.
PIRiB64934.
RefSeqiNP_416260.1. NC_000913.3.
WP_000177206.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76217.
SMRiP76217. Positions 2-484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262236. 13 interactions.
DIPiDIP-9186N.
IntActiP76217. 1 interaction.
STRINGi511145.b1746.

Proteomic databases

EPDiP76217.
PaxDbiP76217.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74816; AAC74816; b1746.
BAA15538; BAA15538; BAA15538.
GeneIDi946260.
KEGGiecj:JW5282.
eco:b1746.
PATRICi32118801. VBIEscCol129921_1818.

Organism-specific databases

EchoBASEiEB3753.
EcoGeneiEG13997. astD.

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271506.
InParanoidiP76217.
KOiK06447.
OMAiMDAAVHL.
OrthoDBiEOG6BS8QW.
PhylomeDBiP76217.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00282.
BioCyciEcoCyc:SUCCGLUALDDEHYD-MONOMER.
ECOL316407:JW5282-MONOMER.
MetaCyc:SUCCGLUALDDEHYD-MONOMER.
BRENDAi1.2.1.3. 2026.

Miscellaneous databases

PROiP76217.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_01174. Aldedh_AstD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR017649. SuccinylGlu_semiald_DH_AstD.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR03240. arg_catab_astD. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli."
    Schneider B.L., Kiupakis A.K., Reitzer L.J.
    J. Bacteriol. 180:4278-4286(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, PATHWAY.
  5. "ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli."
    Kiupakis A.K., Reitzer L.
    J. Bacteriol. 184:2940-2950(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Enzyme genomics: application of general enzymatic screens to discover new enzymes."
    Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F.
    FEMS Microbiol. Rev. 29:263-279(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, KINETIC PARAMETERS.

Entry informationi

Entry nameiASTD_ECOLI
AccessioniPrimary (citable) accession number: P76217
Secondary accession number(s): P78169, P78170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: March 16, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.