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P76215 (ASTE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinylglutamate desuccinylase

EC=3.5.1.96
Gene names
Name:astE
Synonyms:ydjS
Ordered Locus Names:b1744, JW1733
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transforms N(2)-succinylglutamate into succinate and glutamate. Ref.4

Catalytic activity

N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP-Rule MF_00767

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00767

Pathway

Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP-Rule MF_00767

Induction

By nitrogen starvation, and arginine. Induced at stationary phase by sigma S. Ref.6

Sequence similarities

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

acrRP0ACS93EBI-1121806,EBI-1117360

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Succinylglutamate desuccinylase HAMAP-Rule MF_00767
PRO_0000174639

Sites

Active site2101 Potential
Metal binding531Zinc By similarity
Metal binding561Zinc By similarity
Metal binding1471Zinc By similarity

Secondary structure

............................................ 322
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P76215 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 6602BAD75AB0A893

FASTA32235,800
        10         20         30         40         50         60 
MDNFLALTLT GKKPVITERE INGVRWRWLG DGVLELTPLT PPQGALVISA GIHGNETAPV 

        70         80         90        100        110        120 
EMLDALLGAI SHGEIPLRWR LLVILGNPPA LKQGKRYCHS DMNRMFGGRW QLFAESGETC 

       130        140        150        160        170        180 
RARELEQCLE DFYDQGKESV RWHLDLHTAI RGSLHPQFGV LPQRDIPWDE KFLTWLGAAG 

       190        200        210        220        230        240 
LEALVFHQEP GGTFTHFSAR HFGALACTLE LGKALPFGQN DLRQFAVTAS AIAALLSGES 

       250        260        270        280        290        300 
VGIVRTPPLR YRVVSQITRH SPSFEMHMAS DTLNFMPFEK GTLLAQDGEE RFTVTHDVEY 

       310        320 
VLFPNPLVAL GLRAGLMLEK IS 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells."
Hagenmaier S., Stierhof Y.-D., Henning U.
J. Bacteriol. 179:2073-2076(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 92-322.
Strain: K12.
[4]"Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli."
Schneider B.L., Kiupakis A.K., Reitzer L.J.
J. Bacteriol. 180:4278-4286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The Zn-peptidase superfamily: functional convergence after evolutionary divergence."
Makarova K.S., Grishin N.V.
J. Mol. Biol. 292:11-17(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PREDICTION OF ZINC-BINDING RESIDUES AND ACTIVE SITE.
[6]"ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli."
Kiupakis A.K., Reitzer L.
J. Bacteriol. 184:2940-2950(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Crystal structure of succinylglutamate desuccinylase from Escherichia coli, Northeast structural genomics target Et72."
Northeast structural genomics consortium (NESG)
Submitted (MAY-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74814.1.
AP009048 Genomic DNA. Translation: BAE76515.1.
PIRH64933.
RefSeqNP_416258.1. NC_000913.3.
YP_490005.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YW6X-ray3.10A/B1-322[»]
ProteinModelPortalP76215.
SMRP76215. Positions 1-322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP76215. 10 interactions.
STRING511145.b1744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74814; AAC74814; b1744.
BAE76515; BAE76515; BAE76515.
GeneID12933245.
946256.
KEGGecj:Y75_p1719.
eco:b1744.
PATRIC32118797. VBIEscCol129921_1816.

Organism-specific databases

EchoBASEEB3751.
EcoGeneEG13995. astE.

Phylogenomic databases

eggNOGCOG2988.
HOGENOMHOG000280300.
KOK05526.
OMATAPIEIC.
OrthoDBEOG613070.
ProtClustDBPRK05324.

Enzyme and pathway databases

BioCycEcoCyc:SUCCGLUDESUCC-MONOMER.
ECOL316407:JW1733-MONOMER.
MetaCyc:SUCCGLUDESUCC-MONOMER.
UniPathwayUPA00185; UER00283.

Gene expression databases

GenevestigatorP76215.

Family and domain databases

HAMAPMF_00767. Arg_catab_AstE.
InterProIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFPIRSF017020. AstE. 1 hit.
TIGRFAMsTIGR03242. arg_catab_astE. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP76215.
PROP76215.

Entry information

Entry nameASTE_ECOLI
AccessionPrimary (citable) accession number: P76215
Secondary accession number(s): Q2MB41
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene