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P76215

- ASTE_ECOLI

UniProt

P76215 - ASTE_ECOLI

Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Transforms N(2)-succinylglutamate into succinate and glutamate.1 Publication

    Catalytic activityi

    N-succinyl-L-glutamate + H2O = succinate + L-glutamate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531ZincBy similarity
    Metal bindingi56 – 561ZincBy similarity
    Metal bindingi147 – 1471ZincBy similarity
    Active sitei210 – 2101Sequence Analysis

    GO - Molecular functioni

    1. hydrolase activity, acting on ester bonds Source: UniProtKB-HAMAP
    2. protein binding Source: IntAct
    3. succinylglutamate desuccinylase activity Source: EcoCyc
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginine catabolic process to glutamate Source: UniProtKB-HAMAP
    2. arginine catabolic process to succinate Source: UniProtKB-UniPathway
    3. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Arginine metabolism, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:SUCCGLUDESUCC-MONOMER.
    ECOL316407:JW1733-MONOMER.
    MetaCyc:SUCCGLUDESUCC-MONOMER.
    UniPathwayiUPA00185; UER00283.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinylglutamate desuccinylase (EC:3.5.1.96)
    Gene namesi
    Name:astE
    Synonyms:ydjS
    Ordered Locus Names:b1744, JW1733
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13995. astE.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Succinylglutamate desuccinylasePRO_0000174639Add
    BLAST

    Expressioni

    Inductioni

    By nitrogen starvation, and arginine. Induced at stationary phase by sigma S.1 Publication

    Gene expression databases

    GenevestigatoriP76215.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    acrRP0ACS93EBI-1121806,EBI-1117360

    Protein-protein interaction databases

    IntActiP76215. 10 interactions.
    STRINGi511145.b1744.

    Structurei

    Secondary structure

    322
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 1010
    Beta strandi18 – 3013
    Beta strandi33 – 408
    Beta strandi45 – 506
    Beta strandi52 – 554
    Helixi58 – 7114
    Beta strandi79 – 846
    Helixi88 – 914
    Beta strandi105 – 1106
    Helixi117 – 13418
    Beta strandi141 – 1477
    Beta strandi153 – 1619
    Helixi170 – 1789
    Beta strandi182 – 1865
    Helixi194 – 2018
    Beta strandi205 – 2106
    Helixi225 – 23612
    Beta strandi244 – 2463
    Beta strandi249 – 2557
    Beta strandi277 – 2793
    Beta strandi296 – 3027
    Beta strandi316 – 3216

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YW6X-ray3.10A/B1-322[»]
    ProteinModelPortaliP76215.
    SMRiP76215. Positions 1-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP76215.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2988.
    HOGENOMiHOG000280300.
    KOiK05526.
    OMAiTAPIEIC.
    OrthoDBiEOG613070.

    Family and domain databases

    HAMAPiMF_00767. Arg_catab_AstE.
    InterProiIPR007036. Aste_AspA.
    IPR016681. SuccinylGlu_desuccinylase.
    [Graphical view]
    PfamiPF04952. AstE_AspA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017020. AstE. 1 hit.
    TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76215-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNFLALTLT GKKPVITERE INGVRWRWLG DGVLELTPLT PPQGALVISA    50
    GIHGNETAPV EMLDALLGAI SHGEIPLRWR LLVILGNPPA LKQGKRYCHS 100
    DMNRMFGGRW QLFAESGETC RARELEQCLE DFYDQGKESV RWHLDLHTAI 150
    RGSLHPQFGV LPQRDIPWDE KFLTWLGAAG LEALVFHQEP GGTFTHFSAR 200
    HFGALACTLE LGKALPFGQN DLRQFAVTAS AIAALLSGES VGIVRTPPLR 250
    YRVVSQITRH SPSFEMHMAS DTLNFMPFEK GTLLAQDGEE RFTVTHDVEY 300
    VLFPNPLVAL GLRAGLMLEK IS 322
    Length:322
    Mass (Da):35,800
    Last modified:February 1, 1997 - v1
    Checksum:i6602BAD75AB0A893
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74814.1.
    AP009048 Genomic DNA. Translation: BAE76515.1.
    PIRiH64933.
    RefSeqiNP_416258.1. NC_000913.3.
    YP_490005.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74814; AAC74814; b1744.
    BAE76515; BAE76515; BAE76515.
    GeneIDi12933245.
    946256.
    KEGGiecj:Y75_p1719.
    eco:b1744.
    PATRICi32118797. VBIEscCol129921_1816.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74814.1 .
    AP009048 Genomic DNA. Translation: BAE76515.1 .
    PIRi H64933.
    RefSeqi NP_416258.1. NC_000913.3.
    YP_490005.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YW6 X-ray 3.10 A/B 1-322 [» ]
    ProteinModelPortali P76215.
    SMRi P76215. Positions 1-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P76215. 10 interactions.
    STRINGi 511145.b1744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74814 ; AAC74814 ; b1744 .
    BAE76515 ; BAE76515 ; BAE76515 .
    GeneIDi 12933245.
    946256.
    KEGGi ecj:Y75_p1719.
    eco:b1744.
    PATRICi 32118797. VBIEscCol129921_1816.

    Organism-specific databases

    EchoBASEi EB3751.
    EcoGenei EG13995. astE.

    Phylogenomic databases

    eggNOGi COG2988.
    HOGENOMi HOG000280300.
    KOi K05526.
    OMAi TAPIEIC.
    OrthoDBi EOG613070.

    Enzyme and pathway databases

    UniPathwayi UPA00185 ; UER00283 .
    BioCyci EcoCyc:SUCCGLUDESUCC-MONOMER.
    ECOL316407:JW1733-MONOMER.
    MetaCyc:SUCCGLUDESUCC-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P76215.
    PROi P76215.

    Gene expression databases

    Genevestigatori P76215.

    Family and domain databases

    HAMAPi MF_00767. Arg_catab_AstE.
    InterProi IPR007036. Aste_AspA.
    IPR016681. SuccinylGlu_desuccinylase.
    [Graphical view ]
    Pfami PF04952. AstE_AspA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017020. AstE. 1 hit.
    TIGRFAMsi TIGR03242. arg_catab_astE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells."
      Hagenmaier S., Stierhof Y.-D., Henning U.
      J. Bacteriol. 179:2073-2076(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 92-322.
      Strain: K12.
    4. "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli."
      Schneider B.L., Kiupakis A.K., Reitzer L.J.
      J. Bacteriol. 180:4278-4286(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The Zn-peptidase superfamily: functional convergence after evolutionary divergence."
      Makarova K.S., Grishin N.V.
      J. Mol. Biol. 292:11-17(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PREDICTION OF ZINC-BINDING RESIDUES AND ACTIVE SITE.
    6. "ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli."
      Kiupakis A.K., Reitzer L.
      J. Bacteriol. 184:2940-2950(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Crystal structure of succinylglutamate desuccinylase from Escherichia coli, Northeast structural genomics target Et72."
      Northeast structural genomics consortium (NESG)
      Submitted (MAY-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

    Entry informationi

    Entry nameiASTE_ECOLI
    AccessioniPrimary (citable) accession number: P76215
    Secondary accession number(s): Q2MB41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3