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Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transforms N2-succinylglutamate into succinate and glutamate.1 Publication

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase (astA)
  2. N-succinylarginine dihydrolase (astB)
  3. Succinylornithine transaminase (astC)
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53ZincBy similarity1
Metal bindingi56ZincBy similarity1
Metal bindingi147ZincBy similarity1
Active sitei210Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processArginine metabolism, Stress response
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:SUCCGLUDESUCC-MONOMER
MetaCyc:SUCCGLUDESUCC-MONOMER
BRENDAi3.5.1.96 2026
UniPathwayiUPA00185; UER00283

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylase (EC:3.5.1.96)
Gene namesi
Name:astE
Synonyms:ydjS
Ordered Locus Names:b1744, JW1733
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13995 astE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746391 – 322Succinylglutamate desuccinylaseAdd BLAST322

Proteomic databases

EPDiP76215
PaxDbiP76215
PRIDEiP76215

Expressioni

Inductioni

By nitrogen starvation, and arginine. Induced at stationary phase by sigma S.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
acrRP0ACS93EBI-1121806,EBI-1117360

Protein-protein interaction databases

BioGridi4262239, 18 interactors
IntActiP76215, 10 interactors
STRINGi316385.ECDH10B_1882

Structurei

Secondary structure

1322
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 10Combined sources10
Beta strandi18 – 30Combined sources13
Beta strandi33 – 40Combined sources8
Beta strandi45 – 50Combined sources6
Beta strandi52 – 55Combined sources4
Helixi58 – 71Combined sources14
Beta strandi79 – 84Combined sources6
Helixi88 – 91Combined sources4
Beta strandi105 – 110Combined sources6
Helixi117 – 134Combined sources18
Beta strandi141 – 147Combined sources7
Beta strandi153 – 161Combined sources9
Helixi170 – 178Combined sources9
Beta strandi182 – 186Combined sources5
Helixi194 – 201Combined sources8
Beta strandi205 – 210Combined sources6
Helixi225 – 236Combined sources12
Beta strandi244 – 246Combined sources3
Beta strandi249 – 255Combined sources7
Beta strandi277 – 279Combined sources3
Beta strandi296 – 302Combined sources7
Beta strandi316 – 321Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YW6X-ray3.10A/B1-322[»]
ProteinModelPortaliP76215
SMRiP76215
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76215

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107UI5 Bacteria
COG2988 LUCA
HOGENOMiHOG000280300
InParanoidiP76215
KOiK05526
OMAiAHAFTVE

Family and domain databases

CDDicd03855 M14_ASTE, 1 hit
HAMAPiMF_00767 Arg_catab_AstE, 1 hit
InterProiView protein in InterPro
IPR007036 Aste_AspA
IPR016681 SuccinylGlu_desuccinylase
PfamiView protein in Pfam
PF04952 AstE_AspA, 1 hit
PIRSFiPIRSF017020 AstE, 1 hit
TIGRFAMsiTIGR03242 arg_catab_astE, 1 hit

Sequencei

Sequence statusi: Complete.

P76215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNFLALTLT GKKPVITERE INGVRWRWLG DGVLELTPLT PPQGALVISA
60 70 80 90 100
GIHGNETAPV EMLDALLGAI SHGEIPLRWR LLVILGNPPA LKQGKRYCHS
110 120 130 140 150
DMNRMFGGRW QLFAESGETC RARELEQCLE DFYDQGKESV RWHLDLHTAI
160 170 180 190 200
RGSLHPQFGV LPQRDIPWDE KFLTWLGAAG LEALVFHQEP GGTFTHFSAR
210 220 230 240 250
HFGALACTLE LGKALPFGQN DLRQFAVTAS AIAALLSGES VGIVRTPPLR
260 270 280 290 300
YRVVSQITRH SPSFEMHMAS DTLNFMPFEK GTLLAQDGEE RFTVTHDVEY
310 320
VLFPNPLVAL GLRAGLMLEK IS
Length:322
Mass (Da):35,800
Last modified:February 1, 1997 - v1
Checksum:i6602BAD75AB0A893
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74814.1
AP009048 Genomic DNA Translation: BAE76515.1
PIRiH64933
RefSeqiNP_416258.1, NC_000913.3
WP_000368506.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74814; AAC74814; b1744
BAE76515; BAE76515; BAE76515
GeneIDi946256
KEGGiecj:JW1733
eco:b1744
PATRICifig|1411691.4.peg.512

Similar proteinsi

Entry informationi

Entry nameiASTE_ECOLI
AccessioniPrimary (citable) accession number: P76215
Secondary accession number(s): Q2MB41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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