P76213 (CHO_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Excinuclease cho
EC=3.1.25.-

Alternative name(s):
Endonuclease cho
UvrC homolog protein

Gene names

Name:	cho
Synonyms:	ydjQ
Ordered Locus Names:	b1741, JW1730

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than UvrC. Not able to incise the 5' site of a lesion. In vitro, the incision activity of Cho is UvrA and UvrB dependent. When a lesion remains because UvrC is not able to induce the 3' incision, Cho incises the DNA. Then UvrC makes the 5' incision. The combined action of Cho and UvrC broadens the substrate range of nucleotide excision repair. Ref.3
Induction	Repressed by LexA.
Sequence similarities	Contains 1 uvrC C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Molecular function	Excision nuclease Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	nuclease activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	2	EBI-545155,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Excinuclease cho		PRO_0000138370

Sequences

	Sequence		Length	Mass (Da)
	P76213 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: DCEEADD895EF4327	FASTA	295	33,739
	10 20 30 40 50 60 MVRRLTSPRL EFEAAAIYEY PEHLRSFLND LPTRPGVYLF HGESDTMPLY IGKSVNIRSR 70 80 90 100 110 120 VLSHLRTPDE AAMLRQSRRI SWICTAGEIG ALLLEARLIK EQQPLFNKRL RRNRQLCALQ 130 140 150 160 170 180 LNEKRVDVVY AKEVDFSRAP NLFGLFANRR AALQALQTIA DEQKLCYGLL GLEPLSRGRA 190 200 210 220 230 240 CFRSALKRCA GACCGKESHE EHALRLRQSL ERLRVVCWPW QGAVALKEQH PEMTQYHIIQ 250 260 270 280 290 NWLWLGAVNS LEEATTLIRT PAGFDHDGYK ILCKPLLSGN YEITELDPAN DQRAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair." Moolenaar G.F., van Rossum-Fikkert S., van Kesteren M., Goosen N. Proc. Natl. Acad. Sci. U.S.A. 99:1467-1472(2002) [PubMed: 11818552] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74811.1. AP009048 Genomic DNA. Translation: BAE76514.1.
PIR	E64933.
RefSeq	NP_416255.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P76213.
SMR	P76213. Positions 22-109.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-28067N.
IntAct	P76213. 6 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001002; EBESCP00000001002; EBESCG00000000823. EBESCT00000016261; EBESCP00000015552; EBESCG00000015321.
GeneID	948996.
GenomeReviews	Gene locus JW1730 in contig AP009048_GR. Gene locus b1741 in contig U00096_GR.
KEGG	ecj:JW1730. eco:b1741.
PATRIC	32118791. VBIEscCol129921_1813.
Organism-specific databases
EchoBASE	EB3749.
EcoGene	EG13993. cho.
Phylogenomic databases
eggNOG	COG0322.
GeneTree	EBGT00050000011759.
HOGENOM	HBG336547.
OMA	PEHLRPW.
PhylomeDB	P76213.
ProtClustDB	PRK10545.
Enzyme and pathway databases
BioCyc	EcoCyc:G6937-MONOMER.
Gene expression databases
Genevestigator	P76213.
Family and domain databases
InterPro	IPR000305. UvrC_N. [Graphical view]
Gene3D	G3DSA:3.40.1440.10. G3DSA:3.40.1440.10. 1 hit.
KO	K05984.
Pfam	PF01541. GIY-YIG. 1 hit. [Graphical view]
SMART	SM00465. GIYc. 1 hit. [Graphical view]
SUPFAM	SSF82771. UvrC_N. 1 hit.
PROSITE	PS50164. UVRC_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHO_ECOLI

Accession

Primary (citable) accession number: P76213
Secondary accession number(s): Q2MB42

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program