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Protein

Excinuclease cho

Gene

cho

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than UvrC. Not able to incise the 5' site of a lesion. In vitro, the incision activity of Cho is UvrA and UvrB dependent. When a lesion remains because UvrC is not able to induce the 3' incision, Cho incises the DNA. Then UvrC makes the 5' incision. The combined action of Cho and UvrC broadens the substrate range of nucleotide excision repair.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA repair Source: UniProtKB-KW
  • SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease, Hydrolase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:G6937-MONOMER.
ECOL316407:JW1730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Excinuclease cho (EC:3.1.25.-)
Alternative name(s):
Endonuclease cho
UvrC homolog protein
Gene namesi
Name:cho
Synonyms:ydjQ
Ordered Locus Names:b1741, JW1730
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13993. cho.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Excinuclease choPRO_0000138370Add
BLAST

Proteomic databases

PaxDbiP76213.
PRIDEiP76213.

Expressioni

Inductioni

Repressed by LexA.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F52EBI-545155,EBI-543750

Protein-protein interaction databases

BioGridi4261591. 124 interactions.
DIPiDIP-28067N.
IntActiP76213. 7 interactions.
STRINGi511145.b1741.

Structurei

3D structure databases

ProteinModelPortaliP76213.
SMRiP76213. Positions 22-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 10876GIY-YIGPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GIY-YIG domain.PROSITE-ProRule annotation
Contains 1 uvrC C-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG4105DKD. Bacteria.
COG0322. LUCA.
HOGENOMiHOG000121086.
KOiK05984.
OMAiGRCAGAC.
PhylomeDBiP76213.

Family and domain databases

InterProiIPR000305. GIY-YIG_SF.
[Graphical view]
SMARTiSM00465. GIYc. 1 hit.
[Graphical view]
SUPFAMiSSF82771. SSF82771. 1 hit.
PROSITEiPS50164. GIY_YIG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRRLTSPRL EFEAAAIYEY PEHLRSFLND LPTRPGVYLF HGESDTMPLY
60 70 80 90 100
IGKSVNIRSR VLSHLRTPDE AAMLRQSRRI SWICTAGEIG ALLLEARLIK
110 120 130 140 150
EQQPLFNKRL RRNRQLCALQ LNEKRVDVVY AKEVDFSRAP NLFGLFANRR
160 170 180 190 200
AALQALQTIA DEQKLCYGLL GLEPLSRGRA CFRSALKRCA GACCGKESHE
210 220 230 240 250
EHALRLRQSL ERLRVVCWPW QGAVALKEQH PEMTQYHIIQ NWLWLGAVNS
260 270 280 290
LEEATTLIRT PAGFDHDGYK ILCKPLLSGN YEITELDPAN DQRAS
Length:295
Mass (Da):33,739
Last modified:February 1, 1997 - v1
Checksum:iDCEEADD895EF4327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74811.1.
AP009048 Genomic DNA. Translation: BAE76514.1.
PIRiE64933.
RefSeqiNP_416255.1. NC_000913.3.
WP_000252397.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74811; AAC74811; b1741.
BAE76514; BAE76514; BAE76514.
GeneIDi948996.
KEGGiecj:JW1730.
eco:b1741.
PATRICi32118791. VBIEscCol129921_1813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74811.1.
AP009048 Genomic DNA. Translation: BAE76514.1.
PIRiE64933.
RefSeqiNP_416255.1. NC_000913.3.
WP_000252397.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76213.
SMRiP76213. Positions 22-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261591. 124 interactions.
DIPiDIP-28067N.
IntActiP76213. 7 interactions.
STRINGi511145.b1741.

Proteomic databases

PaxDbiP76213.
PRIDEiP76213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74811; AAC74811; b1741.
BAE76514; BAE76514; BAE76514.
GeneIDi948996.
KEGGiecj:JW1730.
eco:b1741.
PATRICi32118791. VBIEscCol129921_1813.

Organism-specific databases

EchoBASEiEB3749.
EcoGeneiEG13993. cho.

Phylogenomic databases

eggNOGiENOG4105DKD. Bacteria.
COG0322. LUCA.
HOGENOMiHOG000121086.
KOiK05984.
OMAiGRCAGAC.
PhylomeDBiP76213.

Enzyme and pathway databases

BioCyciEcoCyc:G6937-MONOMER.
ECOL316407:JW1730-MONOMER.

Miscellaneous databases

PROiP76213.

Family and domain databases

InterProiIPR000305. GIY-YIG_SF.
[Graphical view]
SMARTiSM00465. GIYc. 1 hit.
[Graphical view]
SUPFAMiSSF82771. SSF82771. 1 hit.
PROSITEiPS50164. GIY_YIG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHO_ECOLI
AccessioniPrimary (citable) accession number: P76213
Secondary accession number(s): Q2MB42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.