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Protein

Cysteine desulfuration protein SufE

Gene

sufE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in cysteine desulfuration mediated by SufS. Cysteine desulfuration mobilizes sulfur from L-cysteine to yield L-alanine and constitutes an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Functions as a sulfur acceptor for SufS, by mediating the direct transfer of the sulfur atom from the S-sulfanylcysteine of SufS, an intermediate product of cysteine desulfuration process. Together with the SufBCD complex, it thereby enhances up to 50-fold, the cysteine desulfurase activity of SufS. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Does not affect the selenocysteine lyase activity of SufS.3 Publications

Pathwayi: iron-sulfur cluster biosynthesis

This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Cysteine persulfide intermediateCurated1

GO - Molecular functioni

  • enzyme activator activity Source: EcoCyc
  • sulfur carrier activity Source: EcoCyc

GO - Biological processi

  • iron-sulfur cluster assembly Source: InterPro
  • positive regulation of catalytic activity Source: EcoCyc
  • response to oxidative stress Source: EcoCyc
  • sulfur incorporation into metallo-sulfur cluster Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6905-MONOMER.
ECOL316407:JW1669-MONOMER.
UniPathwayiUPA00266.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfuration protein SufE
Gene namesi
Name:sufE
Synonyms:ynhA
Ordered Locus Names:b1679, JW1669
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13961. sufE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51C → S: Abolishes cysteine desulfurase activity. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002021241 – 138Cysteine desulfuration protein SufEAdd BLAST138

Proteomic databases

PaxDbiP76194.
PRIDEiP76194.

Expressioni

Inductioni

Suf operon is under both the Fe-dependent Fur repressor and the oxidative stress dependent OxyR activator.

Interactioni

Subunit structurei

Homodimer. Interacts with SufS.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
sufSP774443EBI-1124973,EBI-1124981

Protein-protein interaction databases

BioGridi4260278. 38 interactors.
DIPiDIP-10941N.
IntActiP76194. 8 interactors.
STRINGi511145.b1679.

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 16Combined sources10
Helixi20 – 33Combined sources14
Turni39 – 41Combined sources3
Helixi44 – 46Combined sources3
Beta strandi50 – 53Combined sources4
Beta strandi55 – 61Combined sources7
Beta strandi63 – 65Combined sources3
Beta strandi67 – 75Combined sources9
Helixi76 – 88Combined sources13
Turni89 – 91Combined sources3
Helixi94 – 99Combined sources6
Helixi103 – 109Combined sources7
Helixi111 – 114Combined sources4
Helixi117 – 138Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZGX-ray2.00A/B1-138[»]
ProteinModelPortaliP76194.
SMRiP76194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76194.

Family & Domainsi

Sequence similaritiesi

Belongs to the SufE family.Curated

Phylogenomic databases

eggNOGiENOG4105P9C. Bacteria.
COG2166. LUCA.
HOGENOMiHOG000285040.
InParanoidiP76194.
KOiK02426.
OMAiNIIQGCQ.
PhylomeDBiP76194.

Family and domain databases

HAMAPiMF_01832. SufE. 1 hit.
InterProiIPR023939. Cysteine_desulfuration_SufE.
IPR003808. Fe-S_metab-assoc_dom.
[Graphical view]
PfamiPF02657. SufE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLPDKEKL LRNFLRCANW EEKYLYIIEL GQRLPELRDE DRSPQNSIQG
60 70 80 90 100
CQSQVWIVMR QNAQGIIELQ GDSDAAIVKG LIAVVFILYD QMTPQDIVNF
110 120 130
DVRPWFEKMA LTQHLTPSRS QGLEAMIRAI RAKAAALS
Length:138
Mass (Da):15,800
Last modified:February 1, 1997 - v1
Checksum:iAD7B89A74C946772
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74749.1.
AP009048 Genomic DNA. Translation: BAE76500.1.
PIRiG64925.
RefSeqiNP_416194.1. NC_000913.3.
WP_001196530.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74749; AAC74749; b1679.
BAE76500; BAE76500; BAE76500.
GeneIDi946173.
KEGGiecj:JW1669.
eco:b1679.
PATRICi32118664. VBIEscCol129921_1750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74749.1.
AP009048 Genomic DNA. Translation: BAE76500.1.
PIRiG64925.
RefSeqiNP_416194.1. NC_000913.3.
WP_001196530.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZGX-ray2.00A/B1-138[»]
ProteinModelPortaliP76194.
SMRiP76194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260278. 38 interactors.
DIPiDIP-10941N.
IntActiP76194. 8 interactors.
STRINGi511145.b1679.

Proteomic databases

PaxDbiP76194.
PRIDEiP76194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74749; AAC74749; b1679.
BAE76500; BAE76500; BAE76500.
GeneIDi946173.
KEGGiecj:JW1669.
eco:b1679.
PATRICi32118664. VBIEscCol129921_1750.

Organism-specific databases

EchoBASEiEB3719.
EcoGeneiEG13961. sufE.

Phylogenomic databases

eggNOGiENOG4105P9C. Bacteria.
COG2166. LUCA.
HOGENOMiHOG000285040.
InParanoidiP76194.
KOiK02426.
OMAiNIIQGCQ.
PhylomeDBiP76194.

Enzyme and pathway databases

UniPathwayiUPA00266.
BioCyciEcoCyc:G6905-MONOMER.
ECOL316407:JW1669-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76194.
PROiP76194.

Family and domain databases

HAMAPiMF_01832. SufE. 1 hit.
InterProiIPR023939. Cysteine_desulfuration_SufE.
IPR003808. Fe-S_metab-assoc_dom.
[Graphical view]
PfamiPF02657. SufE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUFE_ECOLI
AccessioniPrimary (citable) accession number: P76194
Secondary accession number(s): Q2MB56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.