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Protein

Cysteine desulfuration protein SufE

Gene

sufE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in cysteine desulfuration mediated by SufS. Cysteine desulfuration mobilizes sulfur from L-cysteine to yield L-alanine and constitutes an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Functions as a sulfur acceptor for SufS, by mediating the direct transfer of the sulfur atom from the S-sulfanylcysteine of SufS, an intermediate product of cysteine desulfuration process. Together with the SufBCD complex, it thereby enhances up to 50-fold, the cysteine desulfurase activity of SufS. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Does not affect the selenocysteine lyase activity of SufS.3 Publications

Pathwayi: iron-sulfur cluster biosynthesis

This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511Cysteine persulfide intermediateCurated

GO - Molecular functioni

  • enzyme activator activity Source: EcoCyc
  • sulfur carrier activity Source: EcoCyc

GO - Biological processi

  • iron-sulfur cluster assembly Source: InterPro
  • positive regulation of catalytic activity Source: EcoCyc
  • response to oxidative stress Source: EcoCyc
  • sulfur incorporation into metallo-sulfur cluster Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6905-MONOMER.
ECOL316407:JW1669-MONOMER.
UniPathwayiUPA00266.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfuration protein SufE
Gene namesi
Name:sufE
Synonyms:ynhA
Ordered Locus Names:b1679, JW1669
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13961. sufE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511C → S: Abolishes cysteine desulfurase activity. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 138138Cysteine desulfuration protein SufEPRO_0000202124Add
BLAST

Proteomic databases

PaxDbiP76194.
PRIDEiP76194.

Expressioni

Inductioni

Suf operon is under both the Fe-dependent Fur repressor and the oxidative stress dependent OxyR activator.

Interactioni

Subunit structurei

Homodimer. Interacts with SufS.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
sufSP774443EBI-1124973,EBI-1124981

Protein-protein interaction databases

BioGridi4260278. 38 interactions.
DIPiDIP-10941N.
IntActiP76194. 8 interactions.
STRINGi511145.b1679.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1610Combined sources
Helixi20 – 3314Combined sources
Turni39 – 413Combined sources
Helixi44 – 463Combined sources
Beta strandi50 – 534Combined sources
Beta strandi55 – 617Combined sources
Beta strandi63 – 653Combined sources
Beta strandi67 – 759Combined sources
Helixi76 – 8813Combined sources
Turni89 – 913Combined sources
Helixi94 – 996Combined sources
Helixi103 – 1097Combined sources
Helixi111 – 1144Combined sources
Helixi117 – 13822Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZGX-ray2.00A/B1-138[»]
ProteinModelPortaliP76194.
SMRiP76194. Positions 2-126.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76194.

Family & Domainsi

Sequence similaritiesi

Belongs to the SufE family.Curated

Phylogenomic databases

eggNOGiENOG4105P9C. Bacteria.
COG2166. LUCA.
HOGENOMiHOG000285040.
InParanoidiP76194.
KOiK02426.
OMAiNIIQGCQ.
OrthoDBiEOG61047S.
PhylomeDBiP76194.

Family and domain databases

HAMAPiMF_01832. SufE.
InterProiIPR023939. Cysteine_desulfuration_SufE.
IPR003808. Fe-S_metab-assoc_dom.
[Graphical view]
PfamiPF02657. SufE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLPDKEKL LRNFLRCANW EEKYLYIIEL GQRLPELRDE DRSPQNSIQG
60 70 80 90 100
CQSQVWIVMR QNAQGIIELQ GDSDAAIVKG LIAVVFILYD QMTPQDIVNF
110 120 130
DVRPWFEKMA LTQHLTPSRS QGLEAMIRAI RAKAAALS
Length:138
Mass (Da):15,800
Last modified:February 1, 1997 - v1
Checksum:iAD7B89A74C946772
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74749.1.
AP009048 Genomic DNA. Translation: BAE76500.1.
PIRiG64925.
RefSeqiNP_416194.1. NC_000913.3.
WP_001196530.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74749; AAC74749; b1679.
BAE76500; BAE76500; BAE76500.
GeneIDi946173.
KEGGiecj:JW1669.
eco:b1679.
PATRICi32118664. VBIEscCol129921_1750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74749.1.
AP009048 Genomic DNA. Translation: BAE76500.1.
PIRiG64925.
RefSeqiNP_416194.1. NC_000913.3.
WP_001196530.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZGX-ray2.00A/B1-138[»]
ProteinModelPortaliP76194.
SMRiP76194. Positions 2-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260278. 38 interactions.
DIPiDIP-10941N.
IntActiP76194. 8 interactions.
STRINGi511145.b1679.

Proteomic databases

PaxDbiP76194.
PRIDEiP76194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74749; AAC74749; b1679.
BAE76500; BAE76500; BAE76500.
GeneIDi946173.
KEGGiecj:JW1669.
eco:b1679.
PATRICi32118664. VBIEscCol129921_1750.

Organism-specific databases

EchoBASEiEB3719.
EcoGeneiEG13961. sufE.

Phylogenomic databases

eggNOGiENOG4105P9C. Bacteria.
COG2166. LUCA.
HOGENOMiHOG000285040.
InParanoidiP76194.
KOiK02426.
OMAiNIIQGCQ.
OrthoDBiEOG61047S.
PhylomeDBiP76194.

Enzyme and pathway databases

UniPathwayiUPA00266.
BioCyciEcoCyc:G6905-MONOMER.
ECOL316407:JW1669-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76194.
PROiP76194.

Family and domain databases

HAMAPiMF_01832. SufE.
InterProiIPR023939. Cysteine_desulfuration_SufE.
IPR003808. Fe-S_metab-assoc_dom.
[Graphical view]
PfamiPF02657. SufE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "SufS is a NifS-like protein, and SufD is necessary for stability of the 2Fe-2S FhuF protein in Escherichia coli."
    Patzer S.I., Hantke K.
    J. Bacteriol. 181:3307-3309(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Biogenesis of Fe-S cluster by the bacterial Suf system: SufS and SufE form a new type of cysteine desulfurase."
    Loiseau L., Ollagnier-de-Choudens S., Nachin L., Fontecave M., Barras F.
    J. Biol. Chem. 278:38352-38359(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SUFS, MUTAGENESIS OF CYS-51.
    Strain: K12 / TG1.
  5. "The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli."
    Outten F.W., Wood M.J., Munoz F.M., Storz G.
    J. Biol. Chem. 278:45713-45719(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SUFS, MUTAGENESIS OF CYS-51.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Mechanistic studies of the SufS-SufE cysteine desulfurase: evidence for sulfur transfer from SufS to SufE."
    Ollagnier-de-Choudens S., Lascoux D., Loiseau L., Barras F., Forest E., Fontecave M.
    FEBS Lett. 555:263-267(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SUFS, MUTAGENESIS OF CYS-51.
  7. "The SufE sulfur-acceptor protein contains a conserved core structure that mediates interdomain interactions in a variety of redox protein complexes."
    Goldsmith-Fischman S., Kuzin A., Edstrom W.C., Benach J., Shastry R., Xiao R., Acton T.B., Honig B., Montelione G.T., Hunt J.F.
    J. Mol. Biol. 344:549-565(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiSUFE_ECOLI
AccessioniPrimary (citable) accession number: P76194
Secondary accession number(s): Q2MB56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.