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Protein

Probable L,D-transpeptidase YnhG

Gene

ynhG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible, at least in part, for generating a meso-diaminopimelyl-3-a meso-diaminopimelyl-3 cross-link.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei209Sequence analysis1

GO - Molecular functioni

  • cysteine-type carboxypeptidase activity Source: EcoCyc
  • peptidoglycan L,D-transpeptidase activity Source: EcoCyc
  • transferase activity, transferring amino-acyl groups Source: EcoCyc
  • transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  • cell wall macromolecule metabolic process Source: EcoCyc
  • cell wall organization Source: UniProtKB-KW
  • Gram-negative-bacterium-type cell wall biogenesis Source: EcoCyc
  • peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G6904-MONOMER.
ECOL316407:JW1668-MONOMER.
MetaCyc:G6904-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

MEROPSiC82.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L,D-transpeptidase YnhG (EC:2.-.-.-)
Gene namesi
Name:ynhG
Ordered Locus Names:b1678, JW1668
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14015. ynhG.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Simultaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan. Overexpression of ynhG in this background increases meso-diaminopimelyl-3-a meso-diaminopimelyl-3 cross-links but does not restore Lpp anchoring.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001385524 – 334Probable L,D-transpeptidase YnhGAdd BLAST311

Proteomic databases

EPDiP76193.
PaxDbiP76193.
PRIDEiP76193.

Interactioni

Subunit structurei

Interacts with DsbG.1 Publication

Protein-protein interaction databases

BioGridi4260277. 13 interactors.
DIPiDIP-12774N.
IntActiP76193. 1 interactor.
MINTiMINT-1318911.
STRINGi511145.b1678.

Structurei

3D structure databases

ProteinModelPortaliP76193.
SMRiP76193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 84LysMPROSITE-ProRule annotationAdd BLAST45

Sequence similaritiesi

Belongs to the YkuD family.Curated
Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107N1Q. Bacteria.
COG1376. LUCA.
HOGENOMiHOG000274378.
InParanoidiP76193.
KOiK19234.
OMAiHVIKAKY.
PhylomeDBiP76193.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di3.10.350.10. 1 hit.
InterProiIPR005490. LD_TPept_cat_dom.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF01476. LysM. 1 hit.
PF03734. YkuD. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
PROSITEiPS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRASLLTLT LIGAFSAIQA AWAVDYPLPP TGSRLVGQNQ TYTVQEGDKN
60 70 80 90 100
LQAIARRFDT AAMLILEANN TIAPVPKPGT TITIPSQLLL PDAPRQGIIV
110 120 130 140 150
NLAELRLYYY PPGENIVQVY PIGIGLQGLE TPVMETRVGQ KIPNPTWTPT
160 170 180 190 200
AGIRQRSLER GIKLPPVVPA GPNNPLGRYA LRLAHGNGEY LIHGTSAPDS
210 220 230 240 250
VGLRVSSGCI RMNAPDIKAL FSSVRTGTPV KVINEPVKYS VEPNGMRYVE
260 270 280 290 300
VHRPLSAEEQ QNVQTMPYTL PAGFTQFKDN KAVDQKLVDK ALYRRAGYPV
310 320 330
SVSSGATPAA SNAPSVESAQ NGEPEQGNML RVTQ
Length:334
Mass (Da):36,082
Last modified:February 1, 1997 - v1
Checksum:i25078BB429389D5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74748.1.
AP009048 Genomic DNA. Translation: BAA15458.2.
PIRiF64925.
RefSeqiNP_416193.1. NC_000913.3.
WP_000817105.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74748; AAC74748; b1678.
BAA15458; BAA15458; BAA15458.
GeneIDi946174.
KEGGiecj:JW1668.
eco:b1678.
PATRICi32118662. VBIEscCol129921_1749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74748.1.
AP009048 Genomic DNA. Translation: BAA15458.2.
PIRiF64925.
RefSeqiNP_416193.1. NC_000913.3.
WP_000817105.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76193.
SMRiP76193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260277. 13 interactors.
DIPiDIP-12774N.
IntActiP76193. 1 interactor.
MINTiMINT-1318911.
STRINGi511145.b1678.

Protein family/group databases

MEROPSiC82.A03.

Proteomic databases

EPDiP76193.
PaxDbiP76193.
PRIDEiP76193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74748; AAC74748; b1678.
BAA15458; BAA15458; BAA15458.
GeneIDi946174.
KEGGiecj:JW1668.
eco:b1678.
PATRICi32118662. VBIEscCol129921_1749.

Organism-specific databases

EchoBASEiEB3771.
EcoGeneiEG14015. ynhG.

Phylogenomic databases

eggNOGiENOG4107N1Q. Bacteria.
COG1376. LUCA.
HOGENOMiHOG000274378.
InParanoidiP76193.
KOiK19234.
OMAiHVIKAKY.
PhylomeDBiP76193.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:G6904-MONOMER.
ECOL316407:JW1668-MONOMER.
MetaCyc:G6904-MONOMER.

Miscellaneous databases

PROiP76193.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di3.10.350.10. 1 hit.
InterProiIPR005490. LD_TPept_cat_dom.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF01476. LysM. 1 hit.
PF03734. YkuD. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
PROSITEiPS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYNHG_ECOLI
AccessioniPrimary (citable) accession number: P76193
Secondary accession number(s): P76899, P76900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.