Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Murein DD-endopeptidase MepH

Gene

mepH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Partially suppresses an mepS disruption mutant.1 Publication

Pathwayi: cell wall polysaccharide biosynthesis

This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691NucleophileBy similarity
Active sitei224 – 2241Proton acceptorBy similarity

GO - Molecular functioni

  • cysteine-type peptidase activity Source: UniProtKB-KW
  • endopeptidase activity Source: CACAO

GO - Biological processi

  • capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan metabolic process Source: EcoCyc
  • peptidoglycan turnover Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G6892-MONOMER.
ECOL316407:JW5270-MONOMER.
MetaCyc:G6892-MONOMER.
UniPathwayiUPA00963.

Protein family/group databases

MEROPSiC40.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Murein DD-endopeptidase MepH (EC:3.4.-.-)
Alternative name(s):
Murein hydrolase MepH
Gene namesi
Name:mepH
Synonyms:ydhO
Ordered Locus Names:b1655, JW5270
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13949. mepH.

Pathology & Biotechi

Disruption phenotypei

A triple mepS-mepH-mepM mutant is inviable, whereas a double mepS-mepM will grow on a nutrient-poor medium but not on a rich medium, suggesting the 3 endopeptidases are functionally redundant in vivo. Depletion experiments of the double or triple mutants lead to cell lysis, as well as significantly decreased incorporation of mDAP into peptidogylcan sacculi and increased amounts of the enzyme's substrate (Tetra-Tetra-anhydro muropeptide).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 271244Murein DD-endopeptidase MepHPRO_0000019768Add
BLAST

Proteomic databases

PaxDbiP76190.
PRIDEiP76190.

Interactioni

Protein-protein interaction databases

BioGridi4259398. 202 interactions.
STRINGi511145.b1655.

Structurei

3D structure databases

ProteinModelPortaliP76190.
SMRiP76190. Positions 111-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C40 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105K4H. Bacteria.
COG0791. LUCA.
HOGENOMiHOG000229979.
InParanoidiP76190.
KOiK19303.
OMAiTEKCTPR.
OrthoDBiEOG6GTZPF.
PhylomeDBiP76190.

Family and domain databases

Gene3Di3.90.1720.10. 1 hit.
InterProiIPR000064. NLP_P60_dom.
[Graphical view]
PfamiPF00877. NLPC_P60. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76190-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARINRISIT LCALLFTTLP LTPMAHASKQ ARESSATTHI TKKADKKKST
60 70 80 90 100
ATTKKTQKTA KKAASKSTTK SKTASSVKKS SITASKNAKT RSKHAVNKTA
110 120 130 140 150
SASFTEKCTK RKGYKSHCVK VKNAASGTLA DAHKAKVQKA TKVAMNKLMQ
160 170 180 190 200
QIGKPYRWGG SSPRTGFDCS GLVYYAYKDL VKIRIPRTAN EMYHLRDAAP
210 220 230 240 250
IERSELKNGD LVFFRTQGRG TADHVGVYVG NGKFIQSPRT GQEIQITSLS
260 270
EDYWQRHYVG ARRVMTPKTL R
Length:271
Mass (Da):29,917
Last modified:February 1, 1997 - v1
Checksum:i4B432592D23B31EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74727.1.
AP009048 Genomic DNA. Translation: BAA15421.2.
PIRiA64923.
RefSeqiNP_416172.1. NC_000913.3.
WP_000101193.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74727; AAC74727; b1655.
BAA15421; BAA15421; BAA15421.
GeneIDi945210.
KEGGiecj:JW5270.
eco:b1655.
PATRICi32118614. VBIEscCol129921_1727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74727.1.
AP009048 Genomic DNA. Translation: BAA15421.2.
PIRiA64923.
RefSeqiNP_416172.1. NC_000913.3.
WP_000101193.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76190.
SMRiP76190. Positions 111-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259398. 202 interactions.
STRINGi511145.b1655.

Protein family/group databases

MEROPSiC40.013.

Proteomic databases

PaxDbiP76190.
PRIDEiP76190.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74727; AAC74727; b1655.
BAA15421; BAA15421; BAA15421.
GeneIDi945210.
KEGGiecj:JW5270.
eco:b1655.
PATRICi32118614. VBIEscCol129921_1727.

Organism-specific databases

EchoBASEiEB3707.
EcoGeneiEG13949. mepH.

Phylogenomic databases

eggNOGiENOG4105K4H. Bacteria.
COG0791. LUCA.
HOGENOMiHOG000229979.
InParanoidiP76190.
KOiK19303.
OMAiTEKCTPR.
OrthoDBiEOG6GTZPF.
PhylomeDBiP76190.

Enzyme and pathway databases

UniPathwayiUPA00963.
BioCyciEcoCyc:G6892-MONOMER.
ECOL316407:JW5270-MONOMER.
MetaCyc:G6892-MONOMER.

Miscellaneous databases

PROiP76190.

Family and domain databases

Gene3Di3.90.1720.10. 1 hit.
InterProiIPR000064. NLP_P60_dom.
[Graphical view]
PfamiPF00877. NLPC_P60. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12."
    Singh S.K., SaiSree L., Amrutha R.N., Reddy M.
    Mol. Microbiol. 86:1036-1051(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A MUREIN DD-ENDOPEPTIDASE, DISRUPTION PHENOTYPE.
    Strain: K12.

Entry informationi

Entry nameiMEPH_ECOLI
AccessioniPrimary (citable) accession number: P76190
Secondary accession number(s): P78164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.