P76159 (LYSQ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable lysozyme from lambdoid prophage Qin
EC=3.2.1.17

Alternative name(s):
Endolysin
Lysis protein
Muramidase

Gene names

Name:	arrQ
Synonyms:	ydfQ
Ordered Locus Names:	b1554, JW1546

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity By similarity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 24 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Probable lysozyme from lambdoid prophage Qin		PRO_0000218110
Sites
	Active site	35	1	Proton donor By similarity
	Active site	44	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P76159 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 52DCCA3D315AC888	FASTA	177	19,663
	10 20 30 40 50 60 MNTKIRYGLS AAVLALIGAG ASAPQILDQF LDEKEGNHTM AYRDGSGIWT ICRGATVVDG 70 80 90 100 110 120 KTVFPNMKLS KEKCDQVNAI ERDKALAWVE RNIKVPLTEP QKAGIASFCP YNIGPGKCFP 130 140 150 160 170 STFYKRLNAG DRKGACEAIR WWIKDGGRDC RIRSNNCYGQ VIRRDQESAL TCWGIEQ

References

[1]

"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.

[2]

"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74627.1. AP009048 Genomic DNA. Translation: BAE76469.1.
PIR	E64910.
RefSeq	NP_416072.1. NC_000913.2. YP_489818.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76159.
SMR	P76159. Positions 32-174.
ModBase	Search...
Protein-protein interaction databases
IntAct	P76159. 2 interactions.
STRING	511145.b1554.
Proteomic databases
PRIDE	P76159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74627; AAC74627; b1554. BAE76469; BAE76469; BAE76469.
GeneID	12930507. 946100.
KEGG	ecj:Y75_p1530. eco:b1554.
PATRIC	32118410. VBIEscCol129921_1625.
Organism-specific databases
EchoBASE	EB3590.
EcoGene	EG13829. arrQ.
Phylogenomic databases
eggNOG	COG3772.
HOGENOM	HOG000277067.
KO	K01185.
OMA	QEREGNT.
ProtClustDB	CLSK879915.
Enzyme and pathway databases
BioCyc	EcoCyc:G6827-MONOMER. ECOL316407:JW1546-MONOMER.
Gene expression databases
Genevestigator	P76159.
Family and domain databases
Gene3D	1.10.530.40. 1 hit.
InterPro	IPR002196. Glyco_hydro_24. IPR023346. Lysozyme-like_dom. IPR023347. Lysozyme_dom. [Graphical view]
Pfam	PF00959. Phage_lysozyme. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYSQ_ECOLI

Accession

Primary (citable) accession number: P76159
Secondary accession number(s): Q2MB87

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program