Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable diguanylate cyclase DgcF

Gene

dgcF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules.By similarity

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi181MagnesiumBy similarity1
Metal bindingi182Magnesium; via carbonyl oxygenBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei194Substrate; via carbonyl oxygenBy similarity1
Binding sitei198SubstrateBy similarity1
Metal bindingi224MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6808-MONOMER
UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase DgcFCurated (EC:2.7.7.65By similarity)
Short name:
DGCCurated
Gene namesi
Name:dgcF1 Publication
Synonyms:yneF
Ordered Locus Names:b1522, JW5825
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13814 yneF

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene increases swimming motility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002013361 – 315Probable diguanylate cyclase DgcFAdd BLAST315

Proteomic databases

PaxDbiP76147
PRIDEiP76147

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4260884, 32 interactors
DIPiDIP-47997N
STRINGi316385.ECDH10B_1653

Structurei

3D structure databases

ProteinModelPortaliP76147
SMRiP76147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 310GGDEFPROSITE-ProRule annotationAdd BLAST138

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000120740
InParanoidiP76147
OMAiATWLAWF
PhylomeDBiP76147

Family and domain databases

CDDicd01949 GGDEF, 1 hit
InterProiView protein in InterPro
IPR000160 GGDEF_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00990 GGDEF, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

P76147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLADWFSEQF STGVLIVPCM LTLAIPGVLP RFKAEQMMPA IALIVSVIAS
60 70 80 90 100
VVIGGAGSLA FPLPALIWCA VRYTPQVTCL LTFVTGAVEI VLVANSVIDI
110 120 130 140 150
SVGSPFSIPQ MFSARLGIAT MAICPIMVSF SVAAINSLMK QVALRADFDF
160 170 180 190 200
LTQVYSRSGL YEALKSPSLK QTQHLTVMLL DIDYFKSIND NYGHECGDKV
210 220 230 240 250
LSVFARHIQK IVGDKGLVAR MGGEEFAVAV PSVNPVDGLL MAEKIRKGVE
260 270 280 290 300
LQPFTWQQKT LYLTVSIGVG SGRASYLTLT DDFNKLMVEA DTCLYRSKKD
310
GRNRTSTMRY GEEVV
Length:315
Mass (Da):34,322
Last modified:February 1, 1997 - v1
Checksum:iA4C4AB25E5EA57B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74595.1
AP009048 Genomic DNA Translation: BAA15205.2
PIRiE64906
RefSeqiNP_416039.1, NC_000913.3
WP_000878968.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74595; AAC74595; b1522
BAA15205; BAA15205; BAA15205
GeneIDi947422
KEGGiecj:JW5825
eco:b1522
PATRICifig|1411691.4.peg.744

Entry informationi

Entry nameiDGCF_ECOLI
AccessioniPrimary (citable) accession number: P76147
Secondary accession number(s): P77191
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: February 1, 1997
Last modified: April 25, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health