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Protein

Trans-aconitate 2-methyltransferase

Gene

tam

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities.

Catalytic activityi

S-adenosyl-L-methionine + trans-aconitate = S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate.

GO - Molecular functioni

  • trans-aconitate 2-methyltransferase activity Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6806-MONOMER
MetaCyc:G6806-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-aconitate 2-methyltransferase (EC:2.1.1.144)
Gene namesi
Name:tam
Synonyms:yneD
Ordered Locus Names:b1519, JW1512
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13812 tam

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002180802 – 252Trans-aconitate 2-methyltransferaseAdd BLAST251

Proteomic databases

PaxDbiP76145
PRIDEiP76145

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4260219, 21 interactors
IntActiP76145, 1 interactor
STRINGi316385.ECDH10B_1650

Structurei

3D structure databases

ProteinModelPortaliP76145
SMRiP76145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107R6G Bacteria
COG4106 LUCA
HOGENOMiHOG000125295
InParanoidiP76145
KOiK00598
OMAiTVFPFRR
PhylomeDBiP76145

Family and domain databases

Gene3Di1.10.150.290, 2 hits
HAMAPiMF_00560 Tran_acon_Me_trans, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR023506 Trans-aconitate_MeTrfase
IPR023149 Trans_acon_MeTrfase_C
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDWNPSLYL HFSAERSRPA VELLARVPLE NVEYVADLGC GPGNSTALLQ
60 70 80 90 100
QRWPAARITG IDSSPAMIAE ARSALPDCQF VEADIRNWQP VQALDLIFAN
110 120 130 140 150
ASLQWLPDHY ELFPHLVSLL NPQGVLAVQM PDNWLEPTHV LMREVAWEQN
160 170 180 190 200
YPDRGREPLA GVHAYYDILS EAGCEVDIWR TTYYHQMPSH QAIIDWVTAT
210 220 230 240 250
GLRPWLQDLT ESEQQLFLKR YHQMLEEQYP LQENGQILLA FPRLFIVARR

ME
Length:252
Mass (Da):29,006
Last modified:January 23, 2007 - v3
Checksum:i917BB2F7F1858B4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74592.1
AP009048 Genomic DNA Translation: BAE76459.1
PIRiB64906
RefSeqiNP_416036.1, NC_000913.3
WP_001286597.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74592; AAC74592; b1519
BAE76459; BAE76459; BAE76459
GeneIDi946074
KEGGiecj:JW1512
eco:b1519
PATRICifig|1411691.4.peg.748

Similar proteinsi

Entry informationi

Entry nameiTAM_ECOLI
AccessioniPrimary (citable) accession number: P76145
Secondary accession number(s): Q2MB97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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